MARK1 (AK098103) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MARK; Par-1c; Par1c
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK098103, the custom clone sequence may differ by one or more nucleotides ACCTTCACCCATGGTCCGGAGAGCCTAGCGGGGCTCGCCACCGCCTCCCGGCTCCCCTTCCACGCCTCAT CCTGCCAGCCTCGCCGCCCCGCCAGCGCCGGGCAACCGCCTCGCCCGAAGCCCTCCCTCGTTACTGTCCG CATACCCCGGCGGCGCCGCCGCGGGAAGCGGCTCCCCCGCACCACAGCCCGGCCGGCGAGACCCCGGCCA GACCCCGCTGCCCGCACAAAATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAA TCATACATCTGTGGATGGATATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATC CCCCGGTGTAGAAACTCCATTACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAA AAACAATAGGGAAGGGAAATTTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGC TGTGAAAATAATAGACAAAACTCAGCTAAATCCTACCAGTCTACAAAAGGTATTTAATTAATTTAATAAG TAATTAAAATGTGAGTTTATCATTAATATGTGATGTTCTGTAAAATCAGTCATTCTAAATTTTCTATTAG GTACAATTCTACATTTCATCAATATAAGAAGAAAAGACATAATATAAAATGTAATGTTTATGACTTACCA AGTAGTTGCTTGAAAACTATCTACTACTTAAATACATGTAGTATATGAAAGAAATGTATCTCTTCTATTC AATAGAATAAAACCTTGTGTCATACATTTTGAAGTATTCAGGATTTGAAATATTCAAATATTTTGAAGTA TTTTCAAGTATTCAGAATTGACCTCTGAAAATTAGACTTATATTAAATGAATAATCCATTTACTGATAAT ATCTGGTGCCTTAGCTGTTATATAAAATGACAAATGGTGGTGACCTAAGCCATTTAAACTTTTGTGAGTT TTCAGATAAAGTCCAGAGACCTTGCTGATAAGCTTCTTAATTAAAAATCTAGTTAGAAGTTAATATTGTT CATAGGTGAGTTTCCTTTTATTGCTTCATTTGCTCTTCTTCCTTCTTCCACAAGAACTACTAGTCTTTCA GATAACTCACAGTACAAAATAAGAATGTTTCTTTAAAGGATTGAAAATATGAAACAACATAGAGAGAAAA GAAAGCAGTTAGGAAGGGGATTGCCTTAGCAAACCAAAGCTGTTTCACGTTACTTCATTAATTACTTAAA TGGGAAAATTTTCTCAGATGCACATTTTAGTACTTTAAAAATGAAGCAAATGTTTTAACATTTATGCTGT ATGTGATAGGTTTGTATAGAGTGAGAGAATATACATTGTGACTTTTATTAGAATATAATGGGACCATTGT CACATATGTAATTTTAATACAAATGACTTTGAATTCCTAAAACAGAAAGAATCTTATAAGAAATTGTTAG TATGTGTGCTTACATCTGCTATGATTATAGCATTAGATTAAAAATTTAGATTTGTGATATTCAAACTTTC TTCTCATTTGGCTCAGTCCCAGATTTCTTCTTAGAGCTCTGCCTTTGTACTTATCGGAGATTCTTTTGAA AATTTGACAACCAGTATAACTTTTCATAACACTGATATTTCTGCCCAGTAAGAACATGAAAACTTTGCTG GGTTCTTCTTTTTCTGTGAATGTGAGATAATGTATTATGGGTCTCTTCTTGGAGAAAGTCTCTTCTTTTA GCACCAGTCTTTTTGCTAACCATGATGCCTTTGTGTAAGTGAGAAAAAGACATCCTACCTGGACCAATTA ACTATGCAAAGATGCTGTTTTTTACTGGGCTGGAACCACAGTAGGACATATCTTTGCCTACCCCTGTGCT GGGAACCCACTTAAAATGCACAAACTGGGTAATTGTACACAGTGTCCCTGTCCATGGCCCACATAGCTTT TCTACTTTTCCTGGATTTAGTTGTGGGTTACTTGGCAAATTGAGTGATTTTTAAGTGGCATCTAGAACTA CTCAGGCCTGTTGTGCTAGCTGGAATACACAGCAATGCGACTCCTACTCTGTCTTTCCCAAGGTTGTATA AACAAAGTTGTACTTATTGAAACATGTGAACATGAAACTGAATGTTGCTTTCTTTATCTGAATAACTAAA ACGTGTACTTGATGATGATAAGTAATTTCTGTCATAAAATCTACTAATCTTGTTGATAAAGTATTGCTTT TCCAAATAGGCATATAAAAATGATACAATTTTGTTTTCTTTAACCCGTGAAAATAAAGATGAAGACTCAC T 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK098103 unedited TTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCGCCTCGCCCGAAGC CCTCCCTCGTTACTGTCCGCATACCCCGGCGGCGCCGCCGCGGGAAGCGGCTCCCCCTCC TCTTCCTCCGCGTCCTCTTCCCTCTTTCCCCCGCCGGGGCCGCTTGTTGCACCGCCCCGC GGCCTGCGGGAGCCGCTCGCCCCGGCCTTGTGCTCGCGTCCGCACCCCTTTCCTGTCGCC CCCCGGGGCCCGCACCACAGCCCGGCCGGCGAGACCCCGGCCAGACCCCGCTGCCCGCAC AAAATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACA TCTGTGGATGGATATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAAC ATCCCCCGGTGTAGAAACTCCATTACGTCAGCAACAGATGAACAGCCTCACATTGGAAAT TACCGTTTACAAAAAACAATAGGGAAGGGAAATTTTGCCAAAGTCAAATTGGCAAGACAC GTTCTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAACTCAGCTAAATCCTACC AGTCTACAAAAGGTATTTAATTAATTTAATAAGTAATTAAAATGTGAGTTTATNCATTAA TATGTGATGGTTCTGTAAAATCAGTCATTCTAAATTTTCTATTAGGTACAATTCTACATT TCATCAATATAAGAAGAAAAGACATATATNAAATGGTATGTTTATGACTTACAAAGTAGT TGCTTGAAACTATCTACTACTTAATACATGTAGATATGAAAGAATGTATCTCTTTATTCA TAGATAAACCCTGTGTCTACATTNTGAAGATNCAGGATTNGAAT
Restriction Sites	NotI-NotI
ACCN	AK098103
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK098103.1
RefSeq Size	2311 bp
RefSeq ORF	2311 bp
Locus ID	4139
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.