ATP6V1E1 (NM_001696) Human Untagged Clone

CAT#: SC127436

ATP6V1E1 (untagged)-Human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 1



  "NM_001696" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ATP6V1E1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ARCL2C; ATP6E; ATP6E2; ATP6V1E; P31; Vma4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127436 sequence for NM_001696 edited (data generated by NextGen Sequencing)
ATGGCTCTCAGCGATGCTGACGTGCAAAAGCAGATAAAGCATATGATGGCTTTCATTGAA
CAAGAAGCCAATGAGAAAGCAGAAGAAATAGATGCAAAGGCAGAAGAAGAGTTCAACATA
GAGAAAGGTCGGCTTGTGCAAACCCAAAGACTAAAGATTATGGAATATTATGAGAAGAAA
GAGAAACAGATTGAGCAGCAGAAGAAAATTCAGATGTCCAATTTGATGAATCAAGCGAGA
CTCAAAGTCCTCAGAGCAAGAGATGACCTTATCACAGACCTACTAAATGAAGCAAAACAG
AGACTCAGCAAGGTGGTAAAAGATACAACCAGGTACCAAGTGCTGCTGGATGGACTGGTT
CTCCAGGGTTTGTACCAGTTGCTGGAGCCCCGAATGATTGTTCGTTGCAGGAAACAAGAT
TTCCCTCTGGTAAAGGCTGCAGTGCAGAAGGCAATTCCTATGTACAAAATTGCCACCAAA
AACGATGTTGATGTCCAAATTGACCAGGAGTCCTACCTGCCTGAAGACATAGCTGGTGGA
GTTGAGATCTATAATGGAGATCGTAAAATAAAGGTTTCCAACACCCTGGAAAGCCGGCTG
GATCTCATAGCCCAGCAGATGATGCCAGAAGTCCGGGGAGCCTTGTTTGGTGCAAATGCC
AACAGGAAGTTTTTGGACTAA

Clone variation with respect to NM_001696.3
>OriGene 5' read for NM_001696 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAACCGGAACCCG
CGGTTATAAAGTAAAGGAACCCGAGATCTGCGCAGGGGTTCCCTTTGCCGATTTCTCTCA
CCTCACCTTTCAAACCTAAACTCGAGCCTGCTGTTCACCGGCCTAGCATTGCTCTCGCCA
TGGCTCTCAGCGATGCTGACGTGCAAAAGCAGATAAAGCATATGATGGCTTTCATTGAAC
AAGAAGCCAATGAGAAAGCAGAAGAAATAGATGCAAAGGCAGAAGAAGAGTTCAACATAG
AGAAAGGTCGGCTTGTGCAAACCCAAAGACTAAAGATTATGGAATATTATGAGAAGAAAG
AGAAACAGATTGAGCAGCAGAAGAAAATTCAGATGTCCAATTTGATGAATCAAGCGAGAC
TCAAAGTCCTCAGAGCAAGAGATGACCTTATCACAGACCTACTAAATGAAGCAAAACAGA
GACTCAGCAAGGTGGTAAAAGATACAACCAGGTACCAAGTGCTGCTGGATGGACTGGTTC
TCCAGGGTTTGTACCAGTTGCTGGAGCCCCGAATGATTGTTCGTTGCAGGAAACAAGATT
TCCCTCTGGTAAAGGCTGCAGTGCAGAAGGCAATTCCTATGTACAAAATTGCCACCAAAA
ACGATGTTGATGTCCAAATTGACCAGGAGTCCTACCTGCCTGAAGCATAGCTGTTGGAGT
TGAGATCTATAATGGAGATCGTANAATAAGGTTTCCAACACCCTGGAAAGCCGCTGGATC
TCATAGCCCAGCAGATGATGCCAGAAGTCCGGNGAGCCCTGTTTGGTGCNATGCCACAGG
AAGTTTTGGNACTAAGCCTTCAGAGTGGAGCTCGTCGCAGCTCTCTGCTGTGAGTGGAAG
CN
>OriGene 3' read for NM_001696 unedited
TNCTGGNCTTAGNATTTAAATCACATTTACAGATGAAGGGAAAACTTCTAGGCCTAAACA
GGTAACAGTAAAGCGACTACTGCTGGAAGGAGTGGTGTGCCACATGCGCCTTAAGCCTCA
AGAGTGGGGACTGCAGGGCCAAACACCAAATACATCACCTTTAGGCCAGACGGAGAGTGG
AGACCCAGGAAGCCCATGCAACCACCATCTGCCCCCTCCCCTAGTGCTGCAGAACCGGCT
GGACACTGTCACACTTTCAGAAGAACTGGAGGTGGGTCCTGATCATATTACATGAAGCCT
TCCACCATTGTGAACAATTAGGCAAGGCATGAGTGACAGAGGGGCATCGCTGATAAATAC
AGAGCAATACTGGGGCAGTGAAGAGGAAGCTACAGAGACATTCGTGTTTCTTCAAATATC
AGAAGCTTCCACATCACAGCAGGAGAGCTGACGACGAGCTCCACCTCCTGAAGGCTTAGT
CCAAAAACTTCCTGTTGGCATTTGCACCAAACAAGGCTCCCCGGACTTCTGGCATCATCT
GCTGGGCTATGAGATCCAGCCGGCTTTCCAGGGTGTTGGAAACCTTTATTTTACGATCTC
CATTATAGATCTCAACTCCACCAGCTATGTCTTCAGGCAGGTAGGACTCCTGGTCAATTT
GGACATCAACATCGTTTTTGGTGGCAATTTTGTACATAGGAATTGCCTTCTGCACTGCAG
CCTTTACCAGAGGGAAATCTTGTTTCCCTGCACGAACAATCATTCNGNGCTCCCAGCACT
GGTACANACCCTGGAGAACCAGTCCATCCAGCAGCACTTGGTACCTGGNTGTATCTTTTA
CACCTTG
Restriction Sites NotI-NotI     
ACCN NM_001696
Insert Size 1400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001696.2, NP_001687.1
RefSeq Size 1330 bp
RefSeq ORF 681 bp
Locus ID 529
UniProt ID P36543
Domains vATP-synt_E
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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