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Caldesmon (CALD1) (NM_033139) Human Untagged Clone
CAT＃: SC127344
CALD1 (untagged)-Human caldesmon 1 (CALD1), transcript variant 4
CNY 6,240.00
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_033139, the custom clone sequence may differ by one or more nucleotides
ATGCTGGGTGGATCCGGATCGCATGGAAGACGCAGCCTGGCCGCGCTCTCCCAAATCGCCTACCAGAGGA ATGACGATGATGAAGAGGAGGCAGCCCGGGAACGGCGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAA GCAGGAGGAAGAATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGAC GAGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGGCCGCATTCCTGGAGCGCC TGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGCGGCAGAAGGAGTTCGACCC AACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAATGCAAAATGACACAGCAGAAAATGAAACT ACCGAGAAGGAAGAAAAAAGTGAAAGTCGCCAAGAAAGATACGAGATAGAGGAAACAGAAACAGTCACCA AGTCCTACCAGAAGAATGATTGGAGGGATGCTGAAGAAAACAAGAAAGAAGACAAGGAAAAGGAGGAGGA GGAAGAGGAGAAGCCAAAGCGAGGGAGCATTGGAGAAAATCAGGGAGAAGAGAAGGGAACTAAAGTGCAA GCTAAAAGAGAAAAGCTCCAAGAAGACAAGCCTACCTTCAAAAAAGAAGAGATCAAAGATGAAAAGATTA AAAAGGACAAAGAACCCAAAGAAGAAGTTAAGAGCTTCATGGATCGAAAGAAGGGATTTACAGAAGTTAA GTCGCAGAATGGAGAATTCATGACCCACAAACTTAAACATACTGAGAATACTTTCAGCCGCCCTGGAGGG AGGGCCAGCGTGGACACCAAGGAGGCTGAGGGCGCCCCCCAGGTGGAAGCCGGCAAAAGGCTGGAGGAGC TTCGTCGTCGTCGCGGGGAGACCGAGAGCGAAGAGTTCGAGAAGCTCAAACAGAAGCAGCAGGAGGCGGC TTTGGAGCTGGAGGAACTCAAGAAAAAGAGGGAGGAGAGAAGGAAGGTCCTGGAGGAGGAAGAGCAGAGG AGGAAGCAGGAGGAAGCCGATCGAAAACTCAGAGAGGAGGAAGAGAAGAGGAGGCTAAAGGAAGAGATTG AAAGGCGAAGAGCAGAAGCTGCTGAGAAACGCCAGAAGATGCCAGAAGATGGCTTGTCAGATGACAAGAA ACCATTCAAGTGTTTCACTCCTAAAGGTTCATCTCTCAAGATAGAAGAGCGAGCAGAATTTTTGAATAAG TCTGTGCAGAAAAGCAGTGGTGTCAAATCGACCCATCAAGCAGCAATAGTCTCCAAGATTGACAGCAGAC TGGAGCAGTATACCAGTGCAATTGAGGGAACAAAAAGCGCAAAACCTACAAAGCCGGCAGCCTCGGATCT TCCTGTTCCTGCTGAAGGTGTACGCAACATCAAGAGTATGTGGGAGAAAGGGAATGTGTTTTCATCCCCC ACTGCAGCAGGCACACCAAATAAGGAAACTGCTGGCTTGAAGGTAGGGGTTTCTAGCCGCATCAATGAAT GGCTAACTAAAACCCCAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGAGACGT ATCCAGCAAGCGGAACCTCTGGGAAAAGCAATCTGTGGATAAGGTCACTTCCCCCACTAAGGTTTGA >OriGene 5' read for NM_033139 unedited
GTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGAACGTGTTAA CACAAAGGGGAGAACACGGGAGCATCCTGCACCGTGCATTTCAGCCACAGATCACATGGC CCCTCCGCGGGCCCAGCCCACGGCCTGACCGCCCGGCCTGGCCAGGTCTCCGTATCTCTC TGCCCCGCCGCCCCTTCCCAACTGCGGACATGCTGGGTGGATCCGGATCGCATGGAAGAC GCAGCCTGGCCGCGCTCTCCCAAATCGCCTACCAGAGGAATGACGATGATGAAGAGGAGG CAGCCCGGGAACGGCGCCGCCGAGCCCGACAGGAACGGCTGCGGCAGAAGCAGGAGGAAG AATCCTTGGGACAGGTGACCGACCAGGTGGAGGTGAATGCCCAGAACAGTGTGCCTGACG AGGAGGCCAAGACAACCACCACAAACACTCAAGTGGAAGGGGATGATGAGGCCGCATTCC TGGAGCGCCTGGCTCGGCGTGAGGAAAGACGCCAAAAACGCCTTCAGGAGGCTCTGGAGC GGCAGAAGGAGTTCGACCCAACAATAACAGATGCAAGTCTGTCGCTCCCAAGCAGAAGAA TGCAAAATGACACAGCAGAAAATGAAACTACCGAGAAGGGAGNAAAAAGTGAAAGTCGCC AAGAAAGATACGAGATAGAGGAAACAGAAACAGTCACCAAGTCCTACCAGAAGATGATTG GGAGGATGCTGAAGAAAACAAGAAAGAGACAAGGAAAGGAGGAGGAGGAAAAGGAAAACC CAAGCGAGGGAACCTTGGGAAAAATCCGATCAAGGATGAAAGATTAAAGGGCCAAGAACC CAGGAAAAGTTAAGACTTCATGATCGAAAAAGGGA >OriGene 3' read for NM_033139 unedited
NTTTTAGCTATGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTGCAGGAAGGGAATTCCATTTATTGGGGATGCATTTTCACAATATATGTTT ATTGGAGCGATCCATTATCAGTGAAAAGTATCAAGTGTTTATAAAATTTTTAGGAATGGC AGATTCACAGAACATGCTAGTCAGCTTGCAGTTTTACCTCGTAAAGATAACAGAGAATTA TAGTCAAACCAGTAAACAAGGAATTTACTTTTCAAAAGATTAAATCCAAACTGAACAAAA TTCTACCCTAAAACTTACTCCATCCAAATATTGGAATAAAAGTCAGCAGTGATACATTCT CTTCTGAACTTTAAATTTTCTAGAAAAATATGTAATAGTGATCAGGAGGAGCTCTTGTTC AAAAGTACAACAAAGCAATGTTACCTTACCATAGGCCTTAATTCAAACTTTGATCCATTT CACTCCAATGACGGGAGTCAATGCTACCTGGGACACTTGTATTTGTAAATTCTGATTTAG CTTATTGTAGACTTGTGCCTACTTTGTCATGAGGGTTTGACTTCTGCATTCTTCGTGGCT TTCCTTCCTTTGGCTTAGGTTTGCTAAAGCTAGAAGATTCAATTGCTCTTTACAGACTTA TGAGGAAGATAGACTTTGTAACGCAGATGTCACTTCTCATGCCAGCCCTGCCCTGGTTAG CTCTTCTGGAGGATACTGCAGATAGAAAAATAGTATTTGGGNAGCTCCCTTCATGTGGTA GGATTGAAACTACACAATTTTGTTAAGCCCACTGAGTATGAGTTATAGAACTCCCTGGTT GATTCCGCTTTACTAGACATTTGGCAAAACCTGGAAGACTTTTCATTTATTACTGGAGAA AAAAGACTAAAGCTTTAATAAGTTTGGGTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_033139 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033139.2, NP_149130.1 |
| RefSeq Size | 4291 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 800 |
| UniProt ID | Q05682 |
| Domains | Caldesmon |
| Protein Pathways | Vascular smooth muscle contraction |
| Gene Summary | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. It is mainly expressed in non-muscle tissues or cells. The resulting isoform (4, also known as HeLa l-CaD I) contains a distinct N-terminus and is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214238 | CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,232.00 |
|
| RC214238L1 | Lenti-ORF clone of CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 8,632.00 |
|
| RC214238L2 | Lenti-ORF clone of CALD1 (mGFP-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,460.00 |
|
| RC214238L3 | Lenti-ORF clone of CALD1 (Myc-DDK-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,460.00 |
|
| RC214238L4 | Lenti-ORF clone of CALD1 (mGFP-tagged)-Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 6,460.00 |
|
| RG214238 | CALD1 (tGFP-tagged) - Human caldesmon 1 (CALD1), transcript variant 4 |
CNY 7,832.00 |
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