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PPOX (NM_000309) Human Untagged Clone
CAT＃: SC127285
PPOX (untagged)-Human protoporphyrinogen oxidase (PPOX), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PPO; V290M; VP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127285 sequence for NM_000309 edited (data generated by NextGen Sequencing)
ATGGGCCGGACCGTGGTCGTGCTGGGCGGAGGCATCAGCGGCTTGGCCGCCAGTTACCAC CTGAGCCGGGCCCCCTGCCCCCCTAAGGTGGTCCTAGTGGAGAGCAGTGAGCGTCTGGGA GGCTGGATTCGCTCCGTTCGAGGCCCTAATGGTGCTATCTTTGAGCTTGGACCTCGGGGA ATTAGGCCAGCGGGAGCCCTAGGGGCCCGGACCTTGCTCCTGGTTTCTGAGCTTGGCTTG GATTCAGAAGTGCTGCCTGTCCGGGGAGACCACCCAGCTGCCCAGAACAGGTTCCTCTAC GTGGGCGGTGCCCTGCATGCCCTACCCACTGGCCTCAGGGGGCTACTCCGCCCTTCACCC CCCTTCTCCAAACCTCTGTTTTGGGCTGGGCTGAGGGAGCTGACCAAGCCCCGGGGCAAA GAGCCTGATGAGACTGTGCACAGTTTTGCCCAGCGCCGCCTTGGACCTGAGGTGGCGTCT CTAGCCATGGACAGTCTCTGCCGTGGAGTGTTTGCAGGCAACAGCCGTGAGCTCAGCATC AGGTCCTGCTTTCCCAGTCTCTTCCAAGCTGAGCAAACCCATCGTTCCATATTACTGGGC CTGCTGCTGGGGGCAGGGCGGACCCCACAGCCAGACTCAGCACTCATTCGCCAGGCCTTG GCTGAGCGCTGGAGCCAGTGGTCACTTCGTGGAGGTCTAGAGATGTTGCCTCAGGCCCTT GAAACCCACCTGACTAGTAGGGGGGTCAGTGTTCTCAGAGGCCAGCCGGTCTGTGGGCTC AGCCTCCAGGCAGAAGGGCGCTGGAAGGTATCTCTAAGGGACAGCAGTCTGGAGGCTGAC CACGTTATTAGTGCCATTCCAGCTTCAGTGCTCAGTGAGCTGCTCCCTGCTGAGGCTGCC CCTCTGGCTCGTGCCCTGAGTGCCATCACTGCAGTGTCTGTAGCTGTGGTGAATCTGCAG TACCAAGGAGCCCATCTGCCTGTCCAGGGATTTGGACATTTGGTGCCATCTTCAGAAGAT CCAGGAGTCCTGGGAATCGTGTATGACTCAGTTGCTTTCCCTGAGCAGGACGGGAGCCCC CCTGGCCTCAGAGTGACTGTGATGCTGGGAGGTTCCTGGTTACAGACACTGGAGGCTAGT GGCTGTGTCTTATCTCAGGAGCTGTTTCAACAGCGGGCCCAGGAAGCAGCTGCTACACAA TTAGGACTGAAGGAGATGCCGAGCCACTGCTTGGTCCATCTACACAAGAACTGCATTCCC CAGTATACACTAGGTCACTGGCAAAAACTAGAGTCAGCTAGGCAATTCCTGACTGCTCAC AGGTTGCCCCTGACTCTGGCTGGAGCCTCCTATGAGGGAGTTGCTGTTAATGACTGTATA GAGAGTGGGCGCCAGGCAGCAGTCAGTGTCCTGGGCACAGAACCTAACAGCTGA Clone variation with respect to NM_000309.3 >OriGene 5' read for NM_000309 unedited
TTAGCATTTTGTAATACGACTTACTGATAGGGCGGCCGCGATTCGGCACCAGGGCAAGCA GAGCACCTCAGAACTCAGGCGTACTGCCCGCCGCCCGAGCCCTGCGAGGGCCGATAGCGA GGGTGTGGCCCTTATCTGCACCCAGCAGAGCGCCGGCGGGGTACGGTCTTAGGACCTCGA TCTCCTTCTCCCTCATTTTCTCTCATCCCTACCTATTGTGGGTTTCCGCATGGGCCGGAC CGTGGTCGTGCTGGGCGGAGGCATCAGCGGCTTGGCCGCCAGTTACCACCTGAGCCGGGC CCCCTGCCCCCCTAAGGTGGTCCTAGTGGAGAGCAGTGAGCGTCTGGGAGGCTGGATTCG CTCCGTTCGAGGCCCTAATGGTGCTATCTTTGAGCTTGGACCTCGGGGAATTAGGCCAGC GGGAGCCCTAGGGGCCCGGACCTTGCTCCTGGTTTCTGAGCTTGGCTTGGATTCAGAAGT GCTGCCTGTCCGGGGAGACCACCCAGCTGCCCAGAACAGGGTCCTCTACGTGGGCGGTGC CCTGCATGCCCTACCCACTGGCCTCAGGNGGCTACTCCGCCCTTCACCCCCCTTCTCAAA CCTCTGTTTTGGGCTGGGCTGAGGGAGCTGACCAAGCCCCCGGGCAAAGAGCCTGATGAG ACTGTGCACAGTTTTGCCCAGCGGCCGCCTTGGACCCTGAGNTGGCGTCTCTAGCCATGG GCAGTTTTTTGCCGTGGATTGGTTGCANGCAAACACCCGTGAGCTCAGCATCAGGGTCTG GTTTCCCAGTCTTTTCCAAGCTGACCAAACCCTCGTTCCATATACTGGGCCTGGTTCTGG GGGAGGGGGGACCCCCAGCCGAATCAGCATTATTTCCACGCCTTGGTTGAGCCCGGGCCC ATGGTCACTTTTGGGGGTTAAAAGTTGCCCCAGCCCTT >OriGene 3' read for NM_000309 unedited
NGGGAAGATACGACTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTT TTTTTTTTTTCAACCTCCAGCAATTTTTATTTTCATGAATGAGAGTTGGGGATCAGCTGT TAGGTTCTGTGCCCAGGACACTGACTGCTGCCTGGCGCCCACTCTCTATACAGTCATTAA CAGCAACTCCCTCATAGGAGGCTCCAGCCAGAGTCAGGGGCAACCTGTGAGCAGTCAGGA ATTGCCTAGCTGACTCTAGTTTTTGCCAGTGACCTAGTGTATACTGGGGAATGCAGTTCT TGTGTAGATGGACCAAGCAGTGGCTCGGCATCTCCTTCAGTCCTAATTGTGTAGCAGCTG CTTCCTGGGCCCGCTGTTGAAACAGCTCCTGAGATAAGACACAGCCACTAGCCTCCAGTG TCTGTAACCAGGAACCTCCCAGCATCACAGTCACTCTGAGGCCAGGGGGGCTCCCGTCCT GCTCAGGGAAAGCAACTGAGTCATACACGATTCCCAGGACTCCTGGATCTTCTGAAGATG GCACCAAATGTCCAAATCCCTGGACAGGCAAATGGGCTCCTTGGTCCTGCAGATTCACCA CAGCTACAGACACTGGAGTGATGGGACTTCAGGCACGAGCCAGAGGGGCAGCCCCAGCAG GGAGCAACCCACTGAACACTGAACCTGGAATGGCACTAATAACCGGGGGCAGCCCTCAAA CTGCTGGCCCCTTAAAGATACCTTTCAAACGCCCTTTTTGCCTGGGAGGCTGAACCCCAA AAACCGGCCTGGCCTCTGAAAAACACTGACCCCCCTACTAATTCAGGGGGGGTTTAAAGG GCCTGAGGCAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000309 |
| Insert Size | 1750 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000309.2, NP_000300.1 |
| RefSeq Size | 1703 bp |
| RefSeq ORF | 1434 bp |
| Locus ID | 5498 |
| UniProt ID | P50336 |
| Domains | Amino_oxidase |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Porphyrin and chlorophyll metabolism |
| Gene Summary | This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes isoform 1. Variants 1, 2 and 7 encode the same protein (isoform 1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201788 | PPOX (Myc-DDK-tagged)-Human protoporphyrinogen oxidase (PPOX), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 3,656.00 |
|
| RC201788L3 | Lenti ORF clone of Human protoporphyrinogen oxidase (PPOX), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201788L4 | Lenti ORF clone of Human protoporphyrinogen oxidase (PPOX), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201788 | PPOX (tGFP-tagged) - Human protoporphyrinogen oxidase (PPOX), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,256.00 |
