PEDF (SERPINF1) (M76979) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EPC-1; OI6; OI12; PEDF; PIG35
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC127277 sequence for M76979 edited (data generated by NextGen Sequencing) ATGCAGGCCCTGGTGCTACTCCTCTGCATTGGAGCCCTCCTCGGGCACAGCAGCTGCCAG AACCCTGCCAGCCCCCCGGAGGAGGGCTCCCCAGACCCCGACAGCACAGGGGCGCTGGTG GAGGAGGAGGATCCTTTCTTCAAAGTCCCCGTGAACAAGCTGGCAGCGGCTGTCTCCAAC TTCGGCTATGACCTGTACCGGGTGCGATCCAGCACGAGCCCCACGACCAACGTGCTCCTG TCTCCTCTCAGTGTGGCCACGGCCCTCTCGGCCCTCTCGCTGGGAGCGGANNAGCGAACA GAATCCATCATTCACCGGGCTCTCTACTATGACTTGATCAGCAGCCCAGACATCCATGGT ACCTATAAGGAGCTCCTTGACACGGTCACTGCCCCCCAGAAGAACCTCAAGAGTGCCTCC CGGATCGTCTTTGAGAAGAAGCTGCGCATAAAATCCAGCTTTGTGGCACCTCTGGAAAAG TCATATGGGACCAGGCCCAGAGTCCTGACGGGCAACCCTCGCTTGGACCTGCAAGAGATC AACAACTGGGTGCAGGCGCAGATGAAAGGGAAGCTCGCCAGGTCCACAAAGGAAATTCCC GATGAGATCAGCATTCTCCTTCTCGGTGTGGCGCACTTCAAGGGGCAGTGGGTAACAAAG TTTGACTCCAGAAAGACTTCCCTCGAGGATTTCTACTTGGATGAAGAGAGGACCGTGAGG GTCCCCATGATGTCGGACCCTAAGGCTGTTTTACGCTATGGCTTGGATTCAGATCTCAGC TGCAAGATTGCCCAGCTGCCCTTGACCGGAAGCATGAGTATCATCTTCTTCCTGCCCCTG AAAGTGACCCAGAATTTGACCTTGATAGAGGAGAGCCTCACCTCCGAGTTCATTCATGAC ATAGACCGAGAACTGAAGACCGTGCAGGCGGTCCTCACTGTCCCCAAGCTGAAGCTGAGT TATGAAGGCGAAGTCACCAAGTCCCTGCAGGAGATGAAGCTGCAATCCTTGTTTGATTCA CCAGACTTTAGCAAGATCACAGGCAAACCCATCAAGCTGACTCAGGTGGAACACCGGGCT GGCTTTGAGTGGAACGAGGATGGGGCGGGAACCACCCCCAGCCCAGGGCTGCAGCCTGCC CACCTCACCTTCCCGCTGGACTATCACCTTAACCAGCCTTTCATCTTCGTACTGAGGGAC ACAGACACAGGGGCCCTTCTCTTCATTGGCAAGATTCTGGACCCCAGGGGCCCCTAA Clone variation with respect to M76979.1 215 t=>c;291 c=>n;292 g=>n;444 r=>g;963 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for M76979 unedited TTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGTGTGGAGCTG CAGCGTATCCACAGGCCCCAGGATGCAGGCCCTGGTGCTACTCCTCTGCATTGGAGCCCT CCTCGGGCACAGCAGCTGCCAGAACCCTGCCAGCCCCCCGGAGGAGGGCTCCCCAGACCC CGACAGCACAGGGGCGCTGGTGGAGGAGGAGGATCCTTTCTTCAAAGTCCCCGTGAACAA GCTGGCAGCGGCTGTCTCCAACTTCGGCTATGACCTGTACCGGGTGCGATCCAGCACGAG CCCCACGACCAACGTGCTCCTGTCTCCTCTCAGTGTGGCCACGGCCCTCTCGGCCCTCTC GCTGGGAGCGGAGCAGCGAACAGAATCCATCATTCACCGGGCTCTCTACTATGACTTGAT CAGCAGCCCAGACATCCATGGTACCTATAAGGAGCTCCTTGACACGGTCACTGCCCCCCA GAAGAACCTCAAGAGTGCCTCCCGGATCGTCTTTGAGAAGAAGCTGCGCATAAAATCCAG CTTTGTGGCACCTCTGGAAAAGTCATATGGGACCAGGCCCAGAGTCCTGACGGGCAACCC TCGCTTGGACCTGCAAGAGATCAACAACTGGGTGCAGGCGCAGATGAAAGGGAAGCTCGC CAGGTCCACAAAGGAAATTCCCGATGAGATCAGCATTCTCCTTCTCGGTGTGGCGCACTT CAGGGGGCAGTGGGTAACAAAGTTTGACTCCAGAAAGACTTCCCTCGAGGATNTCTACTT GGATGAAGAGAAGGACCCGTGAGGGTCCNCATGATGTCNGACCCTANGGCTGNNTTACGC TATGGCTGGNATTCAGATCTCAGCTGCAAGATTGCCCAGCTGCCCTTGACCGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for M76979 unedited TAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGGATAAAGCTCTTTTATTGTATGCATTGAA ACCTTACAGGGGCAGCCTTCGTGTCCTGTGGAATCTGCTGTCCCTCGGGTTTTCTTCTAG GGTATTGGAATATTAAACTGGGATATTAGGGGCCCCTGGGGTCCAGAATCTTGCCAATGA AGAGAAGGGCCCCTGTGTCTGTGTCCCTCAGTACGAAGATGAAAGGCTGGTTAAGGTGAT AGTCCAGCGGGAAGGTGAGGTGGGCAGGCTGCAGCCCTGGGCTGGGGGTGGTTCCCGCCC CATCCTCGTTCCACTCAAAGCCAGCCCGGTGTTCCACCTGAGTCAGCTTGATGGGTTTGC CTGTGATCTTGCTAAAGTCTGGTGAATCAAACAAGGATTGCAGCTTCATCTCCTGCAGGG ACTTGGTGACTTCGCCTTCATAACTCAGCTTCAGCTTGGGGACAGTGAGGACCGCCTGCA CGGTCTTCAGTTCTCGGTCTATGTCATGAATGAACTCGGAGGTGAGGCTCTCCTCTATCA AGGTCAAATTCTGGGTCACTTTCAGGNGCANGAAAGAGATGATACTCATGCTTCCGGGTC AGGGCAGCTGGGCAATCTTGCAGCTGAGATCTGAATCCCAGCATAGCGTAAACAGCCTTA GGGTCGACATATGGGACCCTCACGTCTCTCTCATCAGTAAAATCTCGAGGAAGTCTTCTG AGTAAACTTTGTACCACTGCCCTTGAGTGCGCACACGAGAGGAAATGCTGATCTATCGGA ATTNCTTTGTGGACTGGGAGCTTCCTTCATCTGCGCTGACCCATTGTGGACTCTGCAGGT CAACCAGGGTGCCGTCAGACTCTGCCCTGTCATTGACTTTTCAAGTGCACAAGCTGATTT ATGCCACCTCTTTCAAACATCGGAGCCTTTGAGTTCTTGGGGGCNGACGGTAAGACTCTT AGCCCAGAGTTGGCTTGACATCTAAAACCGGGATTAGTTCTTCA
Restriction Sites	NotI-NotI
ACCN	M76979
Insert Size	1420 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	M76979.1, AAA60058.1
RefSeq Size	1490 bp
RefSeq ORF	1257 bp
Locus ID	5176
Domains	SERPIN
Protein Families	Druggable Genome, Secreted Protein
Gene Summary	This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016]

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