Dymeclin (DYM) (AK091256) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DMC; SMC
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC127205 sequence for AK091256 edited (data generated by NextGen Sequencing) ATGGGATCGAATAGCAGCAGAATCGGCGATCTTCCTAAAAATGAGTACTTGAAAAAGTTA TCAGGCACGGAATCTATCTCTGAGAATGACCCGTTCTGGAATCAGCTTCTCTCATTTTCT TTCCCTGCACCAACTAGCAGTAGTGAGTTGAAACTCTTGGAGGAAGCAACCATTTCAGTC TGCAGGTCATTAGTTGAAAACAATCCTCGAACAGGAAATCTTGGTGCACTAATTAAGGTC TTCCTTTCTAGAACCAAAGAACTAAAACTTTCAGCAGAATGTCAGAACCACATCTTCATT TGGCAGACACACAATGCTTTGTTTATTATTTGCTGTTTGCTGAAAGTGTTCATCTGTCAG ATGTCAGAGGAGGAATTACAACTTCATTTTACTTATGAAGAAAAATCTCCTGGCAATTAC AGTTCTGACTCAGAAGATCTTTTGGAAGAATTGCTGTGCTGTTTGATGCAGTTGATCACT GATATTCCACTCTTAGATATTACATATGAAATATCAGTAGAAGCTATATCAACAATGGTT GTTTTCCTTTCCTGCCAACTCTTCCACAAAGAAGTTTTGCGACAGAGCATCAGCCACAAG TATTTGATGCGAGGTCCATGTCTTCCATACACCAGCAAACTTGTGAAGACCTTATTATAT AACTTTATCAGACAAGAAAAGCCACCTCCTCCAGGGGCCCATGTTTTCCCTCAGCAGTCG GATGGGGGAGGACTGCTTTATGGACTTGCATCAGGAGTAGCAACAGGACTCTGGACTGTC TTCACACTAGGTGGTGTGGGCAGCAAAGCGGCTGCCTCTCCAGAGCTTTCTTCCCCTCTG GCCAACCAGAGTCTCCTGCTTCTGCTGGTGTTGGCCAATCTGACAGATGCCTCAGATGCG CCAAACCCCTACAGACAAGCCATTATGTCCTTCAAGAACACACAAGATAGCAGTCCTTTC CCCTCATCAATTCCACATGCCTTCCAGATCAACTTTAATAGTTTGTACACAGCTCTTTGT GAACAGCAGACATCTGATCAAGCAACTCTCCTCTTGTATACCTTGCTCCATCAAAATAGT AATATTAGAACATACATGTTGGCTCGCACAGATATGGAAAATCTTGTTTTACCAATTCTT GAGATTCTGTATCATGTTGAAGAAAGGAATTCACACCATGTGTATATGGCCCTTATAATA TTGTTGATCCTTACGGAAGATGATGGCTTCAACAGATCCATTCATGAAGTGATACTAAAA AATATTACTTGGTATTCAGAACGAGTTTTAACTGAAATCTCCTTGGGGAGTCTCCTGATC CTGGTGGTAATAAGAACCATTCAATACAACATGACTAGGACACGAGACAAGTACCTTCAC ACAAATTGTTTGGCAGCTTTAGCAAATATGTCGGCACAGTTTCGTTCTCTCCATCAGTAT GCTGCCCAGAGGATCATCAGTTTATTTTCTTTGCTGTCTAAAAAACACAACAAAGTTCTG GAACAAGCCACACAGTCCTTGAGAGGTTCGCTGAGTTCTAATGATGTTCCTCTACCAGAT TATGCACAAGACCTAAATGTCATTGAAGAAGTGATTCGAATGATGTTAGAGATCATCAAC TCCTGCCTGACAAATTCCCTTCACCACAACCCAAACTTGGTATACGCCCTGCTTTACAAA CGCGATCTCTTTGAACAATTTCGAACTCATCCTTCATTTCAGGATATAATGCAAAATATT GATCTGGTGATCTCCTTCTTTAGCTCAAGGTTGCTGCAAGCTGGAGCTGAGCTGTCAGTG GAACGGGTCCTGGAAATCATTAAGCAAGGCGTCGTTGCGCTGCCCAAAGACAGACTGAAG AAATTTCCAGAATTGAAATTCAAATATGTGGAAGAGGAGCAGCCCGAGGAGTTTTTTATC CCCTATGTCTGGTCTCTTGTCTACAACTCAGCAGTCGGCCTGTACTGGAATCCACAGGAC ATCCAGCTGTTCACCATGGATTCCGACTGA Clone variation with respect to AK091256.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091256 unedited TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCATCCGTCCGCGGG TCCGTGGAGCTGGAGCAGATCCCCCAGCCGGCTGAGACAGGTTGTCTTTTGGAAATGCAG GTTTAAGGACAAATTATCTGCTTAAGCTAGAAGATGGGATCGAATAGCAGCAGAATCGGC GATCTTCCTAAAAATGAGTACTTGAAAAAGTTATCAGGCACGGAATCTATCTCTGAGAAT GACCCGTTCTGGAATCAGCTTCTCTCATTTTCTTTCCCTGCACCAACTAGCAGTAGTGAG TTGAAACTCTTGGAGGAAGCAACCATTTCAGTCTGCAGGTCATTAGTTGAAAACAATCCT CGAACAGGAAATCTTGGTGCACTAATTAAGGTCTTCCTTTCTAGAACCAAAGAACTAAAA CTTTCAGCAGAATGTCAGAACCACATCTTCATTTGGCAGACACACAATGCTTTGTTTATT ATTTGCTGTTTGCTGAAAGTGTTCATCTGTCAGATGTCAGAGGAGGAATTACAACTTCAT TTTACTTATGAAGAAAAATCTCCTGCNATTTACAGNTTCTGACTCAGAAGATCTTTTGGA AGAATTGCTGCGCTGTTTGAAGCCNTTGAACCCTGATATTCCCCTCTAAAAATTACTTAT GAAATATCCNTACAAGCTATTCACCCCGGGTTGCGTTCCTTTCCTGCCACTGTTGCAACC CCAATTCCCTCTCTCCCCCCCTTCTCCCCCTCCCCCGCTCCAAATCCCCCCCTACCCCCC GTGCCAATTCTCCCCCACGCTCCCCCTTCTCTCTATACTCCACCCCCGCCCCCCCACGCC CCACTCCCCCGCCCTCCCCCCCCCCGTCCCCTCCCACCTTCCCTCTCCCCCCCTCTCG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091256 unedited TGGGTGTTCATCTTATTTTGTTGTGTATTATCCTCCCCTTTTTGCAATACTATCTACGCT GTTTTATCTATTGCCAACTAGCACCAATTCTCCAAATCAAAGTGTGTGAGGAAAACACAC TCGTGCAATCCTCTTTAACAGAAGATACACCAAGTAACCTGTCTGTCTACTTCTGTTACC CAGAAATAAAAGAACTTGAAGGGCTGCTTGGCTGGAGGGGTCCGGGTGGGAGAGCATCCT GCCCTCAGTCGGAATCCATGGTGAACAGCTGGATGTCCTGTGGATTCCAGTACAGGCCGA CTGCTGAGTTGTAGACAAGAGACCAGACATAGGGGATAAAAAACTCCTCGGGCTGCTCCT CTTCCACATATTTGAATTTCAATTCTGGAAATTTCTTCAGTCTGTCTTTGGGCAGCGCAA CGACGCCTTGCTTAATGATTTCCAGGACCCGTTCCACTGACAGCTCAGCTCCAGCTTGCA GCAACCTTGAGCTAAAGAAGGAGATCACCAGATCAATATTTTGCATTATATCCTGAAATG AAGGATGAGTTCGAAATTGTTCAAAGAGATCGCGTTTGTAAAGCAGGGCGTATACCAAGT TTGCGTTGTGGTGAAGGGAATTTGTCACGCAGGAGTTGATGATCTCTACATCATTCGAAT CACTTTTTCAATGACATTTAGGTCCTTGTGCATAATCTGGCACAGGGACATCATTACAAC TCCCCGAACCCTCTCAGGGACTGTGCCGGCTTGTTCCAACACCTTTGGTGCTGTTTTTCA GACAGCAAAGAAAATAACCTGATGATCCTCTGGGCAACATACTGATGGCAAAGACCGAAA CTGCGCCGACTAATTTGCTATAACTGCACAAAATTGGGGGGAAGGCCTTGTTCTCGGTGC TAGCACTGTGGTATCGCAAGGGCCCTTTTACCCTCCGGATCACGAGACTTCTCCAGGGAA CTTCCCGTAACACTCGTTTGTATACCACAAATATTCTATA
Restriction Sites	NotI-NotI
ACCN	AK091256
Insert Size	2560 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK091256.1, BAG52319.1
RefSeq Size	2595 bp
RefSeq ORF	2595 bp
Locus ID	54808
Gene Summary	This gene encodes a protein which regulates Golgi-associated secretory pathways that are essential to endochondral bone formation during early development. This gene is also believed to play a role in early brain development. This gene is widely expressed in embryos and is particularly abundant in chodrocytes and brain tissues. It encodes a peripheral membrane protein which shuttles between the cytosol and Golgi complex. Mutations in this gene are associated with two types of recessive osteochondrodysplasia: Dyggve-Melchior-Clausen (DMC) dysplasia and Smith-McCort (SMC) dysplasia. [provided by RefSeq, Jun 2017]

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