MBD4 (NM_003925) Human Untagged Clone
CAT#: SC127173
MBD4 (untagged)-Human methyl-CpG binding domain protein 4 (MBD4)
CNY 4,328.00
CNY 9,310.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | MED1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_003925, the custom clone sequence may differ by one or more nucleotides
ATGGGCACGACTGGGCTGGAGAGTCTGAGTCTGGGGGACCGCGGAGCTGCCCCCACCGTCACCTCTAGTG AGCGCCTAGTCCCAGACCCGCCGAATGACCTCCGCAAAGAAGATGTTGCTATGGAATTGGAAAGAGTGGG AGAAGATGAGGAACAAATGATGATAAAAAGAAGCAGTGAATGTAATCCCTTGCTACAAGAACCCATCGCT TCTGCTCAGTTTGGTGCTACTGCAGGAACAGAATGCCGTAAGTCTGTCCCATGTGGATGGGAAAGAGTTG TGAAGCAAAGGTTATTTGGGAAGACAGCAGGAAGATTTGATGTGTACTTTATCAGCCCACAAGGACTGAA GTTCAGATCCAAAAGTTCACTTGCTAATTATCTTCACAAAAATGGAGAGACTTCTCTTAAGCCAGAAGAT TTTGATTTTACTGTACTTTCTAAAAGGGGTATCAAGTCAAGATATAAAGACTGCAGCATGGCAGCCCTGA CATCCCATCTACAAAACCAAAGTAACAATTCAAACTGGAACCTCAGGACCCGAAGCAAGTGCAAAAAGGA TGTGTTTATGCCGCCAAGTAGTAGTTCAGAGTTGCAGGAGAGCAGAGGACTCTCTAACTTTACTTCCACT CATTTGCTTTTGAAAGAAGATGAGGGTGTTGATGATGTTAACTTCAGAAAGGTTAGAAAGCCCAAAGGAA AGGTGACTATTTTGAAAGGAATCCCAATTAAGAAAACTAAAAAAGGATGTAGGAAGAGCTGTTCAGGTTT TGTTCAAAGTGATAGCAAAAGAGAATCTGTGTGTAATAAAGCAGATGCTGAAAGTGAACCTGTTGCACAA AAAAGTCAGCTTGATAGAACTGTCTGCATTTCTGATGCTGGAGCATGTGGTGAGACCCTCAGTGTGACCA GTGAAGAAAACAGCCTTGTAAAAAAAAAAGAAAGATCATTGAGTTCAGGATCAAATTTTTGTTCTGAACA AAAAACTTCTGGCATCATAAACAAATTTTGTTCAGCCAAAGACTCAGAACACAACGAGAAGTATGAGGAT ACCTTTTTAGAATCTGAAGAAATCGGAACAAAAGTAGAAGTTGTGGAAAGGAAAGAACATTTGCATACTG ACATTTTAAAACGTGGCTCTGAAATGGACAACAACTGCTCACCAACCAGGAAAGACTTCACTGGTGAGAA AATATTTCAAGAAGATACCATCCCACGAACACAGATAGAAAGAAGGAAAACAAGCCTGTATTTTTCCAGC AAATATAACAAAGAAGCTCTTAGCCCCCCACGACGTAAAGCCTTTAAGAAATGGACACCTCCTCGGTCAC CTTTTAATCTCGTTCAAGAAACACTTTTTCATGATCCATGGAAGCTTCTCATCGCTACTATATTTCTCAA TCGGACCTCAGGCAAAATGGCAATACCTGTGCTTTGGAAGTTTCTGGAGAAGTATCCTTCAGCTGAGGTA GCAAGAACCGCAGACTGGAGAGATGTGTCAGAACTTCTTAAACCTCTTGGTCTCTACGATCTTCGGGCAA AAACCATTGTCAAGTTCTCAGATGAATACCTGACAAAGCAGTGGAAGTATCCAATTGAGCTTCATGGGAT TGGTAAATATGGCAACGACTCTTACCGAATTTTTTGTGTCAATGAGTGGAAGCAGGTGCACCCTGAAGAC CACAAATTAAATAAATATCATGACTGGCTTTGGGAAAATCATGAAAAATTAAGTCTATCTTAA >OriGene 5' read for NM_003925 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGAGCTGAAGAGGT TTCACATCTTACTCCGCCCCACACCCTGGGCGTTGCGGCGCTGGGCTCGTTGCTGCAGCC GGACCCTGCTCGATGGGCACGACTGGGCTGGAGAGTCTGAGTCTGGGGGACCGCGGAGCT GCCCCCACCGTCACCTCTAGTGAGCGCCTAGTCCCAGACCCGCCGAATGACCTCCGCAAA GAAGATGTTGCTATGGAATTGGAAAGAGTGGGAGAAGATGAGGAACAAATGATGATAAAA AGAAGCAGTGAATGTAATCCCTTGCTACAAGAACCCATCGCTTCTGCTCAGTTTGGTGCT ACTGCAGGAACAGAATGCCCCCACAAGGACTGAAGTTCAGATCCAAAAGTTCACTTGCTA ATTATCTTCACAAAAATGGAGAGACTTCTCTTAAGCCAGAAGATTTTGATTTTACTGTAC TTTCTAAAAGGGGTATCAAGTCAAGATATAAAGACTGCAGCATGGCAGCCCTGACATCCC ATCTACAAAACCAAAGTAACAATTCAAACTGGAACCTCAGGACCCGAAGCAAGTGCAAAA AGGATGTGTTTATGCCGCCAAGTAGTAGTTCAGAGTTGCAGGAGAGCAGAGGACTCTCTA ACTTTACTTCCACTCATTTGCTTTTGAAAGAAGATGAGGGTGTTGATGATGTTAACTTCA GAAAGTTAGAAAGCCCAAAGGAAGGTGACTATNTTGAAGGNNATCCNANTAAGANACTAA AAAAGATGTAGGAAGACTGTTCAAGGTTTGNTCAAAGTGATAGCAAAGAGAATCTGTGTG NTATAAAGCAGATGCTGNAAGTGAACCCTGTGCACAAAAAATCAGCTTGATAGAACTGTC TGCATTTCT >OriGene 3' read for NM_003925 unedited
GGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTGTCAAATAATAATTTATT TTAAAAAAATCTCAAAACATGTTCAAACACATTCAGTAGCAAAGATCCACCATTGGCACA CACATTAAGAAAGCACACACACTAGGCTTCTAGTTGGGCTAATTAAAATCTCTATGGCTG GAAAGGTGGTTGGTTGTACTTAATTAAGCTTTTTTGAAGTGCAAAGCTATGCATAACAGA TGAGCTTGAAAGCTGCAGAGTTTAAGATAGACTTAATTTTTCATGATTTTCCCAAAGCCA GTCATGATATTTATTTAATTTGTGGTCTTCAGGGTGCACCTGCTTCCACTCATTGACACA AAAAATTCGGTAAGAGTCGTTGCCATATTTACCAATCCCATGAAGCTCAATTGGATACTT CCACTGCTTTGTCAGGTATTCATCTGAGAACTTGACAATGGTTTTTGCCCGAAGATCGTA GAGACCAAGAGGTTTAAGAAGTTCTGACACATCTCTCCAGTCTGCGGTTCTTGCTACCTC AGCTGAAGGATACTTCTCCAGAAACTTCCAAAGCACAGGTATTGCCATTTTGCCTGAGGT CCGATTGAGAAATATAGTAGCGATGAGAAGCTTCCATGGATCATGAAAAAGTGTTTCTTG AACGAGATTAAAAGGTGACCGANGAGGTGTCCATTTCTTAAAGGCTTTACGTCGTGGGGG GCTAAGAGCTTCTTTGNTATATTTGCTGGAAAAAACANGCTNGNTTTCCTTCTTTCTATC TGNGNTCGNGGGATGGGTATCTCAGTGAAAGTCTTCCTGGNTGGTGAGGCAGTGTTGCCA TTTCAGACCCACGTTTAAATGTCATATGCAAATGTCTTTCTTTCACCACTCTACTTTTGT TCGATTTCTCAGATCTAAAAGGATCCTCATACTCTCGTTGGTTCTGAGCCTTTGCTGACA AAATT |
Restriction Sites | NotI-NotI |
ACCN | NM_003925 |
Insert Size | 2100 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003925.1, NP_003916.1 |
RefSeq Size | 2470 bp |
RefSeq ORF | 1743 bp |
Locus ID | 8930 |
UniProt ID | O95243 |
Domains | MBD |
Protein Families | Druggable Genome |
Protein Pathways | Base excision repair |
Gene Summary | The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223909 | MBD4 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 4 (MBD4) |
CNY 4,320.00 |
|
RC223909L1 | Lenti-ORF clone of MBD4 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 4 (MBD4) |
CNY 6,720.00 |
|
RC223909L2 | Lenti-ORF clone of MBD4 (mGFP-tagged)-Human methyl-CpG binding domain protein 4 (MBD4) |
CNY 6,460.00 |
|
RC223909L3 | Lenti-ORF clone of MBD4 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 4 (MBD4) |
CNY 6,720.00 |
|
RC223909L4 | Lenti-ORF clone of MBD4 (mGFP-tagged)-Human methyl-CpG binding domain protein 4 (MBD4) |
CNY 6,460.00 |
|
RG223909 | MBD4 (tGFP-tagged) - Human methyl-CpG binding domain protein 4 (MBD4) |
CNY 5,920.00 |