MBD4 (NM_003925) Human Untagged Clone

CAT#: SC127173

MBD4 (untagged)-Human methyl-CpG binding domain protein 4 (MBD4)



  "NM_003925" in other vectors (6)

CNY 4,328.00

CNY 9,310.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal MBD4 Antibody
    • 100 ul

CNY 5,381.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MED1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003925, the custom clone sequence may differ by one or more nucleotides


ATGGGCACGACTGGGCTGGAGAGTCTGAGTCTGGGGGACCGCGGAGCTGCCCCCACCGTCACCTCTAGTG
AGCGCCTAGTCCCAGACCCGCCGAATGACCTCCGCAAAGAAGATGTTGCTATGGAATTGGAAAGAGTGGG
AGAAGATGAGGAACAAATGATGATAAAAAGAAGCAGTGAATGTAATCCCTTGCTACAAGAACCCATCGCT
TCTGCTCAGTTTGGTGCTACTGCAGGAACAGAATGCCGTAAGTCTGTCCCATGTGGATGGGAAAGAGTTG
TGAAGCAAAGGTTATTTGGGAAGACAGCAGGAAGATTTGATGTGTACTTTATCAGCCCACAAGGACTGAA
GTTCAGATCCAAAAGTTCACTTGCTAATTATCTTCACAAAAATGGAGAGACTTCTCTTAAGCCAGAAGAT
TTTGATTTTACTGTACTTTCTAAAAGGGGTATCAAGTCAAGATATAAAGACTGCAGCATGGCAGCCCTGA
CATCCCATCTACAAAACCAAAGTAACAATTCAAACTGGAACCTCAGGACCCGAAGCAAGTGCAAAAAGGA
TGTGTTTATGCCGCCAAGTAGTAGTTCAGAGTTGCAGGAGAGCAGAGGACTCTCTAACTTTACTTCCACT
CATTTGCTTTTGAAAGAAGATGAGGGTGTTGATGATGTTAACTTCAGAAAGGTTAGAAAGCCCAAAGGAA
AGGTGACTATTTTGAAAGGAATCCCAATTAAGAAAACTAAAAAAGGATGTAGGAAGAGCTGTTCAGGTTT
TGTTCAAAGTGATAGCAAAAGAGAATCTGTGTGTAATAAAGCAGATGCTGAAAGTGAACCTGTTGCACAA
AAAAGTCAGCTTGATAGAACTGTCTGCATTTCTGATGCTGGAGCATGTGGTGAGACCCTCAGTGTGACCA
GTGAAGAAAACAGCCTTGTAAAAAAAAAAGAAAGATCATTGAGTTCAGGATCAAATTTTTGTTCTGAACA
AAAAACTTCTGGCATCATAAACAAATTTTGTTCAGCCAAAGACTCAGAACACAACGAGAAGTATGAGGAT
ACCTTTTTAGAATCTGAAGAAATCGGAACAAAAGTAGAAGTTGTGGAAAGGAAAGAACATTTGCATACTG
ACATTTTAAAACGTGGCTCTGAAATGGACAACAACTGCTCACCAACCAGGAAAGACTTCACTGGTGAGAA
AATATTTCAAGAAGATACCATCCCACGAACACAGATAGAAAGAAGGAAAACAAGCCTGTATTTTTCCAGC
AAATATAACAAAGAAGCTCTTAGCCCCCCACGACGTAAAGCCTTTAAGAAATGGACACCTCCTCGGTCAC
CTTTTAATCTCGTTCAAGAAACACTTTTTCATGATCCATGGAAGCTTCTCATCGCTACTATATTTCTCAA
TCGGACCTCAGGCAAAATGGCAATACCTGTGCTTTGGAAGTTTCTGGAGAAGTATCCTTCAGCTGAGGTA
GCAAGAACCGCAGACTGGAGAGATGTGTCAGAACTTCTTAAACCTCTTGGTCTCTACGATCTTCGGGCAA
AAACCATTGTCAAGTTCTCAGATGAATACCTGACAAAGCAGTGGAAGTATCCAATTGAGCTTCATGGGAT
TGGTAAATATGGCAACGACTCTTACCGAATTTTTTGTGTCAATGAGTGGAAGCAGGTGCACCCTGAAGAC
CACAAATTAAATAAATATCATGACTGGCTTTGGGAAAATCATGAAAAATTAAGTCTATCTTAA


>OriGene 5' read for NM_003925 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGAGCTGAAGAGGT
TTCACATCTTACTCCGCCCCACACCCTGGGCGTTGCGGCGCTGGGCTCGTTGCTGCAGCC
GGACCCTGCTCGATGGGCACGACTGGGCTGGAGAGTCTGAGTCTGGGGGACCGCGGAGCT
GCCCCCACCGTCACCTCTAGTGAGCGCCTAGTCCCAGACCCGCCGAATGACCTCCGCAAA
GAAGATGTTGCTATGGAATTGGAAAGAGTGGGAGAAGATGAGGAACAAATGATGATAAAA
AGAAGCAGTGAATGTAATCCCTTGCTACAAGAACCCATCGCTTCTGCTCAGTTTGGTGCT
ACTGCAGGAACAGAATGCCCCCACAAGGACTGAAGTTCAGATCCAAAAGTTCACTTGCTA
ATTATCTTCACAAAAATGGAGAGACTTCTCTTAAGCCAGAAGATTTTGATTTTACTGTAC
TTTCTAAAAGGGGTATCAAGTCAAGATATAAAGACTGCAGCATGGCAGCCCTGACATCCC
ATCTACAAAACCAAAGTAACAATTCAAACTGGAACCTCAGGACCCGAAGCAAGTGCAAAA
AGGATGTGTTTATGCCGCCAAGTAGTAGTTCAGAGTTGCAGGAGAGCAGAGGACTCTCTA
ACTTTACTTCCACTCATTTGCTTTTGAAAGAAGATGAGGGTGTTGATGATGTTAACTTCA
GAAAGTTAGAAAGCCCAAAGGAAGGTGACTATNTTGAAGGNNATCCNANTAAGANACTAA
AAAAGATGTAGGAAGACTGTTCAAGGTTTGNTCAAAGTGATAGCAAAGAGAATCTGTGTG
NTATAAAGCAGATGCTGNAAGTGAACCCTGTGCACAAAAAATCAGCTTGATAGAACTGTC
TGCATTTCT
>OriGene 3' read for NM_003925 unedited
GGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTGTCAAATAATAATTTATT
TTAAAAAAATCTCAAAACATGTTCAAACACATTCAGTAGCAAAGATCCACCATTGGCACA
CACATTAAGAAAGCACACACACTAGGCTTCTAGTTGGGCTAATTAAAATCTCTATGGCTG
GAAAGGTGGTTGGTTGTACTTAATTAAGCTTTTTTGAAGTGCAAAGCTATGCATAACAGA
TGAGCTTGAAAGCTGCAGAGTTTAAGATAGACTTAATTTTTCATGATTTTCCCAAAGCCA
GTCATGATATTTATTTAATTTGTGGTCTTCAGGGTGCACCTGCTTCCACTCATTGACACA
AAAAATTCGGTAAGAGTCGTTGCCATATTTACCAATCCCATGAAGCTCAATTGGATACTT
CCACTGCTTTGTCAGGTATTCATCTGAGAACTTGACAATGGTTTTTGCCCGAAGATCGTA
GAGACCAAGAGGTTTAAGAAGTTCTGACACATCTCTCCAGTCTGCGGTTCTTGCTACCTC
AGCTGAAGGATACTTCTCCAGAAACTTCCAAAGCACAGGTATTGCCATTTTGCCTGAGGT
CCGATTGAGAAATATAGTAGCGATGAGAAGCTTCCATGGATCATGAAAAAGTGTTTCTTG
AACGAGATTAAAAGGTGACCGANGAGGTGTCCATTTCTTAAAGGCTTTACGTCGTGGGGG
GCTAAGAGCTTCTTTGNTATATTTGCTGGAAAAAACANGCTNGNTTTCCTTCTTTCTATC
TGNGNTCGNGGGATGGGTATCTCAGTGAAAGTCTTCCTGGNTGGTGAGGCAGTGTTGCCA
TTTCAGACCCACGTTTAAATGTCATATGCAAATGTCTTTCTTTCACCACTCTACTTTTGT
TCGATTTCTCAGATCTAAAAGGATCCTCATACTCTCGTTGGTTCTGAGCCTTTGCTGACA
AAATT
Restriction Sites NotI-NotI     
ACCN NM_003925
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003925.1, NP_003916.1
RefSeq Size 2470 bp
RefSeq ORF 1743 bp
Locus ID 8930
UniProt ID O95243
Domains MBD
Protein Families Druggable Genome
Protein Pathways Base excision repair
Gene Summary The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Transcript Variant: This variant (1) encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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