D4 (ARHGDIB) (NM_001175) Human Untagged Clone

CAT#: SC127108

ARHGDIB (untagged)-Human Rho GDP dissociation inhibitor (GDI) beta (ARHGDIB)



  "NM_001175" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


ARHGDIB rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127108 sequence for NM_001175 edited (data generated by NextGen Sequencing)
ATGACTGAAAAAGCCCCAGAGCCACATGTGGAGGAGGATGACGATGATGAGCTGGACAGC
AAGCTCAATTATAAGCCTCCACCACAGAAGTCCCTGAAAGAGCTGCAGGAAATGGACAAA
GATGATGAGAGTCTAATTAAGTACAAGAAAACGCTGCTGGGAGATGGTCCTGTGGTGACA
GATCCGAAAGCCCCCAATGTCGTTGTCACCCGGCTCACCCTGGTTTGTGAGAGTGCCCCG
GGACCAATCACCATGGACCTTACTGGAGATCTGGAAGCCCTCAAAAAGGAAACCATTGTG
TTAAAGGAAGGTTCTGAATATAGAGTCAAAATTCACTTCAAAGTGAACAGGGATATTGTG
TCAGGCCTGAAATACGTTCAGCACACCTACAGGACTGGGGTGAAAGTGGATAAAGCAACA
TTTATGGTTGGCAGCTATGGACCTCGGCCTGAGGAGTATGAGTTCCTCACTCCAGTTGAG
GAGGCTCCCAAGGGCATGCTGGCCCGAGGCACGTACCACAACAAGTCCTTCTTCACCGAC
GATGACAAGCAAGACCACCTCAGCTGGGAGTGGAACCTGTCGATTAAGAAGGAGTGGACA
GAATGA

Clone variation with respect to NM_001175.4
42 t=>c;504 g=>c
>OriGene 5' read for NM_001175 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGACGTGAAGCACTG
AATAAATAGATCAGAATGACTGAAAAAGCCCCAGAGCCACATGTGGAGGAGGATGACGAT
GATGAGCTGGACAGCAAGCTCAATTATAAGCCTCCACCACAGAAGTCCCTGAAAGAGCTG
CAGGAAATGGACAAAGATGATGAGAGTCTAATTAAGTACAAGAAAACGCTGCTGGGAGAT
GGTCCTGTGGTGACAGATCCGAAAGCCCCCAATGTCGTTGTCACCCGGCTCACCCTGGTT
TGTGAGAGTGCCCCGGGACCAATCACCATGGACCTTACTGGAGATCTGGAAGCCCTCAAA
AAGGAAACCATTGTGTTAAAGGAAGGTTCTGAATATAGAGTCAAAATTCACTTCAAAGTG
AACAGGGATATTGTGTCAGGCCTGAAATACGTTCAGCACACCTACAGGACTGGGGTGAAA
GTGGATAAAGCAACATTTATGGTTGGCAGCTATGGACCTCGGCCTGAGGAGTATGAGTTC
CTCACTCCAGTTGAGGAGGCTCCCAAGGGCATGCTGGCCCGAGGCACGTACCACAACAAG
TCCTTCTTCACCGACGATGACAAGCAAGACCACCTCAGCTGGGAGTGGAACCTGTCGATT
AAGAAAGAGTGGACAGAATGAATGCATCCACCCCTTTCCCCACCGTTGCCACCTGNAAGA
AATCTCTCAGGGCGTGTCAGCACCCTGTCCCTTCTCCCTGTCACAGCTGGGTCCCTCTTC
ACACTGCCACATTNCTTATGATGCATCTTTNCCACCTGTCACTCACGTGGTCCTANAAAC
AGAGCTAAANCGGNCTTNCACCACTGCTCCTTGATCTNCATAGGNCNATCTTCCAGTCTC
ANCTAGACACATCATTATAATTCCTGGCTACCCTATCAGCACTAAGCCCAAAATGGGCAT
ATACTAAGGCTTNGACTAGTNNCGANTCATCATGCTAATTCTTTGGGTGTGCTGCCAAGG
CN
Restriction Sites NotI-NotI     
ACCN NM_001175
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001175.4, NP_001166.3
RefSeq Size 1216 bp
RefSeq ORF 606 bp
Locus ID 397
UniProt ID P52566
Domains Rho_GDI
Protein Families Druggable Genome
Protein Pathways Neurotrophin signaling pathway
Gene Summary Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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