TPR (AK023111) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK023111, the custom clone sequence may differ by one or more nucleotides GAGAGAGAATGTTAAAGGATGAAGTTTCCAAATGTGTATGTCGCTGTGAAGATCTGGAGAAACAAAACAG ATTACTTCATGATCAGATCGAAAAATTAAGTGACAAGGTCGTTGCCTCTGTGAAGGAAGGTGTACAAGGT CCACTGAATGTATCTCTCAGTGAAGAAGGAAAATCTCAAGAACAAATTTTGGAAATTCTCAGATTTATAC GACGAGAAAAAGAAATTGCTGAAACTAGGTTTGAGGTGGCTCAGGTTGAGAGTCTGCGTTATCGACAAAG GGTTGAACTTTTAGAAAGAGAGCTGCAGGAACTGCAAGATAGTCTAAATGCTGAAAGGGAGAAAGTCCAG GTAGGTATACTAAGCTTTAAGAAAGCACTTGTTAAATCAAAAGAGAAGCACTCATTTATATCTCTACCAT TTAACGTAATGTAAAATTTAACTTGAAGTAAGCATTCTTCTAAACTTGTAAATTCATTAAGCATTTGTTA AACACTAATATAGTATAGTGCTTAAAAGTAAAGGCTCTGTAACCAAACTGCCTGGTTTCAGATTCTAGCT TCATACCATTAGCTGTGGCCTTGGGTACATTGTTTAACTTCCCTGCTCTTAGTTTCTTCATCTGTAATAT TTGGATAATAATGAGGTTTAATTGAGTTTGTGCACATGAAGCATTTAGAAGCATGTCTGGTATGTAGTGG GTGCTTAATAAATTCCAGCTATCTTAATTTATTATAGTTATGGATTATACGTGTGGATAGTCATTCTAGG ATAAAGTGATTTAAACTGCTACAGAATATCTTTCTCACCCAAGGAGCAAAAAGTAGTAAAAACTAACAAC CAAAGGAAAAGAAGCTGTGGTAGTGAAATGGATGATAATAAGACAAGCTTGACTCCTTACCCCACACTAC CACCCAAAGCTTATTGTTGAGTTACTTCATTCTTAGGAGCTACAGTAATATACCCCCACCTCTAGAAGGA AGGAAATTAAGATCTTAAAACATTTATCTTCTTTTAACTGATGAGTAGTCAAAATGACTGTTAAGAAGAA ACCATTGAACAGGGAAAAGTAAATAATGAATTTTATGGGATTTATGTGTTCAGCAAAAGCTAAAGGTTCC AGAATTATATCCTGTTTAATTCAAATGTATCTCACCATGGAAATAATAGAGCCTCCAGTTCAATAGAGTT AATACTTGGAGGAAAAGGGACAGAATCAGAGTCTCTAACAAAGACTGCATATTGTTTCAGTCTTTTCCGC TTCATTTTTTATTCCTTTGGGCTATAGAAAGTAAATACTAGAAAATGCCCGCCTACGAATGAGTTCATAG GGGGAAACCAACTCTTAAATACAGGAAGAAGAGAGTCAACAAGTAACATAGATTATATAAAAAATAATAT AATGGGCCGGGCATGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACGA GGTTAGGAGATTGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAATTA GCTGGGCATGGTGGTGGCAGGCACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAACGGCGTAA ATCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCACACCACTGGACTCCAGTCTGGGCGACAGAGCGAGA CTCCGTCTCAAAAAAAAAAAAAAAAATTATAATAGCAATGTAATGTCTGTCTAAGCATGAACAGTGTAGC ATGGCAACCACGAAATATGCTATAGCTTTAAAAACAGTGGGGGAAGAAAGTAATACCAAGTGCAGAAGAT GGATAATTATAACCTATGAGAAGGAAAAAATTCTTCACAGATAATTCAGTAAAATTTCTCATGGCCAAAG TGATGAAACAATGGATCAAAATGAGAGATTTCTGAACAAGAGGGAATAAACTGAGGATTAGAATAACATT TTAGAACTAATAAATTTAGTGCAGCAAGGAAAAAAAGATACAGAAAGGTAAATAGAGATTTGGATTAATG TTTTTACCTAATGTTAATACTTTGTTGGTGGGATTTGTAGCATCTTTTCACTTTTGTCTTTGTAATTCTT GTATTTGTTTAAAGTCTGCAGTTCGCATGCCTCTCAGAAAAGTAAACGTGAACTTTCAATAATTTTAGTT CTAAAAGTTGAAACTTAAATCCTCTTTATTTTGTCATAGGGAGGATTTGAGCTCATAGGAACAGGTAGAC TTTCAACTATTGTTAGATGTTGGTATGTATATTTATTTGAAATGTTTAGGTAACTGCAAAAACAATGGCT CAGCATGAAGAACTGATGAAGAAAACTGAAACAGTGAATGTAGTTATGGAGACCAATAAAATGCTAAGAG AAGAGAAGGAGAGACTAGAACAGGATCTACAGCAAATGCAAGCAAAGGTGAGGAAACTGGAGTTAGATAT TTTACCCTTACAAGAAGCAAATGCTGAGCTGAGTGAGAAAAGCGGTATGTTGCAGGCAGAGAAGAAGCTC TTAGAAGAGGATGTCAAACGTTGGAAAGCACGTAACCAGCATCTAGTAAGTCAACAGAAAGATCCAGATA CAGAAGAATATCGGAAGCTCCTTTCTGAAAAGGAAGTTCATACTAAGCGTATTCAACAATTGACAGAAGA AATTGGTAGACTTAAAGCTGAAATTGCAAGATCAAATGCATCTTTGACTAACAACCAGAACTTAATTCAG AGTCTGAAGGAAGATCT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK023111 unedited TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCG GCACGAGGGAAAGTCCAGGTAACTGCAAAAACAATGGCTCAGCATGAAGAACTGATGAAG AAAACTGAAACAATGAATGTAGTTATGGAGACCAATAAAATGCTAAGAGAAGAGAAGGAG AGACTAGAACAGGATCTACAGCAAATGCAAGCAAAGGTGAGGAAACTGGAGTTAGATATT TTACCCTTACAAGAAGCAAATGCTGAGCTGAGTGAGAAAAGCGGTATGTTGCAGGCAGAG AAGAAGCTCTTAGAAGAGGATGTCAAACGTTGGAAAGCACGTAACCAGCATCTAGTAAGT CAACAGAAAGATCCAGATACAGAAGAATATCGGAAGCTCCTTTCTGAAAAGGAAGTTCAT ACTAAGCGTATTCAACAATTGACAGAAGAAATTGGTAGACTTAAAGCTGAAATTGCAAGA TCAAATGCATCTTTGACTAACAACCAGAACTTAATTCAGAGTCTGAAGGAAGATCTAAAT AAAGTAAGAACTGAAAAGGAAACCATCCAGAAGGACTTAGATGCCAAAATAATTGATATC CAAGAAAAAGTCAAAACTATNTACTCAAGTTAAGAAAATTGGNNACGTANGTACCAAGAC TCAATATNGAAGAACTTAANAGCACAACAGGNATAANGGTTATGGNAGACATCGNGCTCA GTCCTCTGGGAGACATCANGAGCAGCATGGTTTCAGTCCAGGAATGCAGGAACTCAAAGA ACGCTCAACCAAGCTGAACAAATCAAAATCACTTGAAAGTCAGTAGAGAATTGCAGAAGA CATATCTGAAAAGAGAAGAGCANGAATCTCAGAACAATGGGCACTTATCTGACTTTACGA CTGGNNAGATCTAGGAAAAACCCAGAGGGGCGCTTCGACACGATACGAA
Restriction Sites	NotI-NotI
ACCN	AK023111
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK023111.1
RefSeq Size	2747 bp
RefSeq ORF	2747 bp
Locus ID	7175
Protein Pathways	Pathways in cancer, Thyroid cancer
Gene Summary	This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq, Jul 2008]

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