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Lipoamide Dehydrogenase (DLD) (NM_000108) Human Untagged Clone
CAT＃: SC126940
DLD (untagged)-Human dihydrolipoamide dehydrogenase (DLD)
CNY 3,800.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DLDD; DLDH; E3; GCSL; LAD; OGDC-E3; PHE3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126940 sequence for NM_000108 edited (data generated by NextGen Sequencing)
ATGCAGAGCTGGAGTCGTGTGTACTGCTCCTTGGCCAAGAGAGGCCATTTCAATCGAATA TCTCATGGCCTACAGGGACTTTCTGCAGTGCCTCTGAGAACTTACGCAGATCAGCCGATT GATGCTGATGTAACAGTTATAGGTTCTGGTCCTGGAGGATATGTTGCTGCTATTAAAGCT GCCCAGTTAGGCTTCAAGACAGTCTGCATTGAGAAAAATGAAACACTTGGTGGAACATGC TTGAATGTTGGTTGTATTCCTTCTAAGGCTTTATTGAACAACTCTCATTATTACCATATG GCCCATGGAAAAGATTTTGCATCTAGAGGAATTGAAATGTCCGAAGTTCGCTTGAATTTA GACAAGATGATGGAGCAGAAGAGTACTGCAGTAAAAGCTTTAACAGGTGGAATTGCCCAC TTATTCAAACAGAATAAGGTTGTTCATGTCAATGGATATGGAAAGATAACTGGCAAAAAT CAAGTCACTGCTACGAAAGCTGATGGCGGCACTCAGGTTATTGATACAAAGAACATTCTT ATAGCCACGGGTTCAGAAGTTACTCCTTTTCCTGGAATCACGATAGATGAAGATACAATA GTGTCATCTACAGGTGCTTTATCTTTAAAAAAAGTTCCAGAAAAGATGGTTGTTATTGGT GCAGGAGTAATAGGTGTAGAATTGGGTTCAGTTTGGCAAAGACTTGGTGCAGATGTGACA GCAGTTGAATTTTTAGGTCATGTAGGTGGAGTTGGAATTGATATGGAGATATCTAAAAAC TTTCAACGCATCCTTCAAAAACAGGGGTTTAAATTTAAATTGAATACAAAGGTTACTGGT GCTACCAAGAAGTCAGATGGAAAAATTGATGTTTCTATTGAAGCTGCTTCTGGTGGTAAA GCTGAAGTTATCACTTGTGATGTACTCTTGGTTTGCATTGGCCGACGACCCTTTACTAAG AATTTGGGACTAGAAGAGCTGGGAATTGAACTAGATCCCAGAGGTAGAATTCCAGTCAAT ACCAGATTTCAAACTAAAATTCCAAATATCTATGCCATTGGTGATGTAGTTGCTGGTCCA ATGCTGGCTCACAAAGCAGAGGATGAAGGCATTATCTGTGTTGAAGGAATGGCTGGTGGT GCTGTGCACATTGACTACAATTGTGTGCCATCAGTGATTTACACACACCCTGAAGTTGCT TGGGTTGGCAAATCAGAAGAGCAGTTGAAAGAAGAGGGTATTGAGTACAAAGTTGGGAAA TTCCCATTTGCTGCTAACAGCAGAGCTAAGACAAATGCTGACACAGATGGCATGGTGAAG ATCCTTGGGCAGAAATCGACAGACAGAGTACTGGGAGCACATATTCTTGGACCAGGTGCT GGAGAAATGGTAAATGAAGCTGCTCTTGCTTTGGAATATGGAGCATCCTGTGAAGATATA GCTAGAGTCTGTCATGCACATCCGACCTTATCAGAAGCTTTTAGAGAAGCAAATCTTGCT GCGTCATTTGGCAAATCAATCAACTTTTGA Clone variation with respect to NM_000108.3 >OriGene 5' read for NM_000108 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCAGCGGAGGT GAAAGTTTGGCGGAAAGGAAAAACAGCGGAAAAAGCAGAGCTGGAGTCGTGTGTACTGCT CCTTGGCCAAGAGAGGCCATTTCAATCGAATATCTCATGGCCTACAGGGACTTTCTGCAG TGCCTCTGAGAACTTACGCAGATCAGCCGATTGATGCTGATGTAACAGTTATAGGTTCTG GTCCTGGAGGATATGTTGCTGCTATTAAAGCTGCCCAGTTAGGCTTCAAGACAGTCTGCA TTGAGAAAAATGAAACACTTGGTGGAACATGCTTGAATGTTGGTTGTATTCCTTCTAAGG CTTTATTGAACAACTCTCATTATTACCATATGGCCCATGGAAAAGATTTTGCATCTAGAG GAATTGAAATGTCCGAAGTTCGCTTGAATTTAGACAAGATGATGGAGCAGAAGAGTACTG CAGTAAAAGCTTTAACAGGTGGAATTGCCCACTTATTCAAACAGAATAAGGTTGTTCATG TCAATGGATATGGAAAGATAACTGGCAAAAATCAAGTCACTGCTACGAAAGCTGATGGCG GCACTCAGGTTATTGATACAAAGAACATTCTTATAGCCACGGGTTCAGAAGTTACTCCTT TTCCTGGAATCACGATAGATGAAGATACAATAGTGTCATCTACAGGTGCTTTATCTTTAA AAAAAGTTCCAGAAAAGATGGTTGTTATTGGTGCAGGAGTAATAGGTGTAGAAATTGGGT TCAGTTTGGCAAAGACTTGGTGCAGATGTGACAGCAGTTGAATTTTTAGGTCATGTAGGT GGGAGTGGNAATGATATGGNAGATATCTAAAAACTTCACN >OriGene 3' read for NM_000108 unedited
TTTTTTTTTTTTTTTTTAACCAGTAAATGAATATTTAAGACGTTTATTTCAGTATCTTCA TTCCCCACTGTAATACATTTGAATGCCCACGTATTTGAGTTCCGTAAATAGACTCTCTTA ATTTGGGTAACTATATAATTACCCTTCAGAGTTGCAGACAACAATGGAAGCTTTAAAACC TCTTCAACACAAATGCTACCCCTAAAATGAAAGAATTTAGAGGTTAAATAAAACAAGTGA GAGACCATTTACTTACATCAGTTCGGTTTATAGACATTTGAATCATATCTGAATGACTGA CTTGTTTCCAATGTGAAAACCAAATTAAAAATAACTTGATCACTGTGCTTCAAACACAAC TGTTCACATATCAAATTCTAGCTCCAGCCATGTAAAATTCAACTTTTCTGTATCAGAGAA AAAAGCTATTTAAATTCAGGGGCATTGAATCTTTCATGAACAGCATGAAAATATGAGTTG ACGGAGATATTCCAGGATGCTACAATAAGTAGCTTTTTGTCTTACATATTAGTGCACTGA AACAAAATACTAAATTTATGTAAAATATAGATATAAGAGTATTCCATTTTGTCCAAACCT ATTAAATACCTTCCAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000108 |
| Insert Size | 2190 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000108.2, NP_000099.1 |
| RefSeq Size | 2320 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 1738 |
| UniProt ID | P09622 |
| Domains | pyr_redox, pyr_redox_dim |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation |
| Gene Summary | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Stimulation of reactive oxygen species generation by disease-causing mutations of lipoamide dehydrogenase
,Attila Ambrus, Beata Torocsik, Laszlo Tretter, Oliver Ozohanics, and Vera Adam-Vizi,
Hum. Mol. Genet., May 2011; 10.1093/hmg/ddr202
[Dld]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200639 | DLD (Myc-DDK-tagged)-Human dihydrolipoamide dehydrogenase (DLD) |
CNY 3,792.00 |
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| RC200639L1 | Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), Myc-DDK-tagged |
CNY 6,192.00 |
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| RC200639L2 | Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), mGFP tagged |
CNY 5,890.00 |
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| RC200639L3 | Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), Myc-DDK-tagged |
CNY 5,890.00 |
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| RC200639L4 | Lenti ORF clone of Human dihydrolipoamide dehydrogenase (DLD), mGFP tagged |
CNY 5,890.00 |
|
| RG200639 | DLD (tGFP-tagged) - Human dihydrolipoamide dehydrogenase (DLD) |
CNY 5,392.00 |
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