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EFEMP1 (NM_004105) Human Untagged Clone
CAT＃: SC126895
EFEMP1 (untagged)-Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1
CNY 5,488.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DHRD; DRAD; EGF-containing fibulin-like extracellular matrix protein 1; FBLN3; FBNL; fibrillin-like; fibulin 3; FLJ35535; MGC111353; MLVT; MTLV; S1-5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126895 sequence for NM_004105 edited (data generated by NextGen Sequencing)
ATGTTGAAAGCCCTTTTCCTAACTATGCTGACTCTGGCGCTGGTCAAGTCACAGGACACC GAAGAAACCATCACGTACACGCAATGCACTGACGGATATGAGTGGGATCCTGTGAGACAG CAATGCAAAGATATTGATGAATGTGACATTGTCCCAGACGCTTGTAAAGGTGGAATGAAG TGTGTCAACCACTATGGAGGATACCTCTGCCTTCCGAAAACAGCCCAGATTATTGTCAAT AATGAACAGCCTCAGCAGGAAACACAACCAGCAGAAGGAACCTCAGGGGCAACCACCGGG GTTGTAGCTGCCAGCAGCATGGCAACCAGTGGAGTGTTGCCCGGGGGTGGTTTTGTGGCC AGTGCTGCTGCAGTCGCAGGCCCTGAAATGCAGACTGGCCGAAATAACTTTGTCATCCGG CGGAACCCAGCTGACCCTCAGCGCATTCCCTCCAACCCTTCCCACCGTATCCAGTGTGCA GCAGGCTACGAGCAAAGTGAACACAACGTGTGCCAAGACATAGACGAGTGCACTGCAGGG ACGCACAACTGTAGAGCAGACCAAGTGTGCATCAATTTACGGGGATCCTTTGCATGTCAG TGCCCTCCTGGATATCAGAAGCGAGGGGAGCAGTGCGTAGACATAGATGAATGTACCATC TCTCCATATTGCCACCAAAGATGCGTGAATACACCAGGCTCATTTTATTGCCAGTGCAGT CCTGGGTTTCAATTGGCAGCAAACAACTATACCTGCGTAGATATAAATGAATGTGATGCC AGCAATCAATGTGCTCAGCAGTGCTACAACATTCTTGGTTCATTCATCTGTCAGTGCAAT CAAGGATATGAGCTAAGCAGTGACAGGCTCAACTGTGAAGACATTGATGAATGCAGAACC TCAAGCTACCTGTGTCAATATCAATGTGTCAATGAACCTGGGAAATTCTCATGTATGTGC CCCCAGGGATACCAAGTGGTGAGAAGTAGAACATGTCAAGATATAAATGAGTGTGAGACC ACAAATGAATGCCGGGAGGATGAAATGTGTTGGAATTATCATGGCGGCTTCCGTTGTTAT CCACGAAATCCTTGTCAAGATCCCTACATTCTAACACCAGAGAACCGATGTGTTTGCCCA GTCTCAAATGCCATGTGCCGAGAACTGCCCCAGTCAATAGTCTACAAATACATGAGCATC CGATCTGATAGGTCTGTGCCATCAGACATCTTCCAGATACAGGCCACAACTATTTATGCC AACACCATCAATACTTTTCGGATTAAATCTGGAAATGAAAATGGAGAGTTCTACCTACGA CAAACAAGTCCTGTAAGTGCAATGCTTGTGCTCGTGAAGTCATTATCAGGACCAAGAGAA CATATCGTGGACCTGGAGATGCTGACAGTCAGCAGTATAGGGACCTTCCGCACAAGCTCT GTGTTAAGATTGACAATAATAGTGGGGCCATTTTCATTTTAG Clone variation with respect to NM_004105.3 661 c=>t >OriGene 5' read for NM_004105 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCTGCCCTCCCCT GCGCACTCCCCTCGCTGCCCGGGCCCGGAGCGCAGCGCGGCCGCACAGATTCACAATGTT GAAAGCCCTTTTCCTAACTATGCTGACTCTGGCGCTGGTCAAGTCACAGGACACCGAAGA AACCATCACGTACACGCAATGCACTGACGGATATGAGTGGGATCCTGTGAGACAGCAATG CAAAGATATTGATGAATGTGACATTGTCCCAGACGCTTGTAAAGGTGGAATGAAGTGTGT CAACCACTATGGAGGATACCTCTGCCTTCCGAAAACAGCCCAGATTATTGTCAATAATGA ACAGCCTCAGCAGGAAACACAACCAGCAGAAGGAACCTCAGGGGCAACCACCGGGGTTGT AGCTGCCAGCAGCATGGCAACCAGTGGAGTGTTGCCCGGGGGTGGTTTTGTGGCCAGTGC TGCTGCAGTCGCAGGCCCTGAAATGCAGACTGGCCGAAATAACTTTGTCATCCGGCGGAA CCCAGCTGACCCTCAGCGCATTCCCTCCAACCCTTCCCACCGTATCCAGTGTGCAGCAGG CTACGAGCAAAGTGAACACAACGTGTGCCAAGACATAGACGAGTGCACTGCANGGACGCA CAACTGTAGAGCAGACCAAGTGTGCATCAATTACGGGGATCCTTTGCATGTCAGTGCCCT CCTGGATATCANAAGCGAGGGGAGCAGTGCGTAAACATAGATGAATGTACCATCTCTTCA TATTGCCACCAAAGATGCGTGAATACACCANGCTCATTTTATTTGCAGTGCAGTCCTGGG TTTCAATTGGCAGCAACAACTATACCTGCGTAGATATAATGAATGGGATGCCAGCAATCA TGTGCTCAGCAGTGCTACAACATTCTTGTTCATCATCTGTCATGCAAN >OriGene 3' read for NM_004105 unedited
TGACCGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTCTAAAGAAATTATCTTT ATTGACCTTTTCCATTTTCTTACAAAGCAGTTAAAAACTCATTCTTACCCTTGCATGCTA TTCAATGGTTATGATGGCTGCCTCCTTATGAGACTGTTAGTGGAGCTCTAGTAAGTTTCC TTAAGCACCACAGAAGATCATCATTGCTTGGTCCCACTTTTATAGGAAAGAATCCAAGTT TCACCAGATAGTGTATACTTAGCTCTCTTGAAGAAGACCAGTTTAGTGGATTAATGTCTA ATTTACATATAGTAATAAAGACAAACTTTGAATCTTTACATATTAAATGCCCACTTTATA CCATGGTGTAATTGTTTGAATTATGGGTGAGTGTACAGTATAGAGATGTAGATGCACTAG ATATATCTATAAATAAAATAGGGCTTTAAGGTAACAATATTCTTTGGCTGACTTAAATGC CTGTGGTTGACTCTTAGAAAAGACTAAAATGAAAATGGCCCCACTATTATTGTCAATCTT AACACAGAGCTTGTGCGGAAGGTCCCTATACTGCTGACTGTCAGCATCTCCAGGTCCACG ATATGTTCTCTTGGTCCTGATAATGACTTCACGAGCACAAGCATTGCACTTACAGGACTT GTTTGTCGTAGGTAGAACTCTCCATTTTCATTTCCAGATTTAATCCCGAAAGTATTGATG GGTGTGGCATAAATAGTTGTAGCCCTGTATCTGGAAAATGTCTGATGCACANACCTATCA AATCGGATGCTCATGTATTTGTANACTATTGACTGGGGCAGTTCTGGCACATGGCATTTG AGACTGGGCAAACACATCGGTCCTTTGGGGTAAAATGTAGGGATCTGACAGGNATTTCCT GGGTAACACCNAAGCCGCCTGGAATTTCCACCATTTATTCTCCCCGCGTTCCTTGGGGGC AAACCCATATTTATTGGATGTGTTCCCTTCACCCCTGGGNTNCCGGGGGGCCCTCTGGAA TATTCACGGTCTTGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004105 |
| Insert Size | 2050 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004105.2, NP_004096.2 |
| RefSeq Size | 2742 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 2202 |
| Domains | EGF_CA, EGF, EGF |
| Protein Families | Secreted Protein |
| Gene Summary | This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The EFEMP1 Gene: A Frequent Target for Epigenetic Silencing in Multiple Human Pituitary Adenoma Subtypes
,Duong, CV;Yacqub-Usman, K;Emes, RD;Clayton, RN;Farrell, WE;,
Neuroendocrinology. 2013 Nov 6
,PubMed ID 24080855
[EFEMP1]
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EFEMP1 Expression Promotes In vivo Tumor Growth in Human Pancreatic Adenocarcinoma
,Hendrik Seeliger, Peter Camaj, Ivan Ischenko, Axel Kleespies, Enrico N. De Toni, Susanne E. Thieme, Helmut Blum, Gerald Assmann, Karl-Walter Jauch, and Christiane J. Bruns,
Mol. Cancer Res., Feb 2009; 7: 189 - 198.
[EFEMP1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214953 | EFEMP1 (Myc-DDK-tagged)-Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1 |
CNY 5,488.00 |
|
| RC214953L1 | Lenti ORF clone of Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC214953L2 | Lenti ORF clone of Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1, mGFP tagged |
CNY 7,520.00 |
|
| RC214953L3 | Lenti ORF clone of Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC214953L4 | Lenti ORF clone of Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG214953 | EFEMP1 (tGFP-tagged) - Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1 |
CNY 7,088.00 |
