B7H3 (CD276) (BC011578) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	4Ig-B7-H3; B7-H3; B7H3; B7RP-2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC011578, the custom clone sequence may differ by one or more nucleotides CAGCCCTGCAGCCTCTGAAACACTCTGACAGCAAAGAAGATGATGGACAAGAAATAGCCTGACCATGAGG ACCAGGGAGCTGCTACCCCTCCCTACAGCTCCTACCCTCTGGCTGCAATGGGGCTGCACTGTGAGCCCTG CCCCCAACAGATGCATCCTGCTCTGACAGGTGGGCTCCTTCTCCAAAGGATGCGATACACAGACCACTGT GCAGCCTTATTTCTCCAATGGACATGATTCCCAAGTCATCCTGCTGCCTTTTTTTCTTATAGACACAATG AACAGACCACCCACAACCTTAGTTCTCTAAGTCATCCTGCCTGCTGCCTTATTTCACAGTACATACATTT CTTAGGGACACAGTACACTGACCACATCACCACCCTCTTCTTCCAGTGCTGCGTGGACCATCTGGCTGCC TTTTTTCTCCAAAAGATGCAATATTCAGACTGACTGACCCCCTGCCTTATTTCACCAAAGACACGATGCA TAGTCACCCCGGCCTTGTTTCTCCAATGGCCGTGATACACTAGTGATCATGTTCAGCCCTGCTTCCACCT GCATAGAATCTTTTCTTCTCAGACAGGGACAGTGCGGCCTCAACATCTCCTGGAGTCTAGAAGCTGTTTC CTTTCCCCTCCTTCCTCCTCTTGCTCTAGCCTTAATACTGGCCTTTTCCCTCCCTGCCCCAAGTGAAGAC AGGGCACTCTGCGCCCACCACATGCACAGCTGTGCATGGAGACCTGCAGGTGCACGTGCTGGAACACGTG TGGTTCCCCCCTGGCCCAGCCTCCTCTGCAGTGCCCCTCTCCCCTGCCCATCCTCCCCACGGAAGCATGT GCTGGTCACACTGGTTCTCCAGGGGTCTGTGATGGGGCCCCTGGGGGTCAGCTTCTGTCCCTCTGCCTTC TCACCTCTTTGTTCCTTTCTTTTCATGTATCCATTCAGTTGATGTTTATTGAGCAACTACAGATGTCAGC ACTGTGTTAGGTGCTGGGGGCCCTGCGTGGGAAGATAAAGTTCCTCCCTCAAGGACTCCCCATCCAGCTG GGAGACAGACAACTAACTACACTGCACCCTGCGGTTTGCAGGGGGCTCCTGCCTGGCTCCCTGCTCCACA CCTCCTCTGTGGCTCAAGGCTTCCTGGATACCTCACCCCCATCCCACCCATAATTCTTACCCAGAGCATG GGGTTGGGGCGGAAACCTGGAGAGAGGGACATAGCCCCTCGCCACGGCTAGAGAATCTGGTGGTGTCCAA AATGTCTGTCCAGGTGTGGGCAGGTGGGCAGGCACCAAGGCCCTCTGGACCTTTCATAGCAGCAGAAAAG GCAGAGCCTGGGGCAGGGCAGGGCCAGGAATGCTTTGGGGACACCGAGGGGACTGCCCCCCACCCCCACC ATGGTGCTATTCTGGGGCTGGGGCAGTCTTTTCCTGGCTTGCCTCTGGCCAGCTCCCGGCCTCTGGTAGA GTGAGACTTCAGACGTTCTGATGCCTTCCGGATGTCATCTCTCCCTGCCCCAGGAATGGAAGATGTGAGG ACTTCTAATTTAAATGTGGGACTCGGAGGGATTTTGTAAACTGGGGGTATATTTTGGGGAAAATAAATGT CTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011578 unedited CCGCGAAATTCGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGTACACTGACCACATCA CCACCCTCTTCTTCCAGTGCTGCGTGGACCATCTGGCTGCCTTTTTTCTCCAAAAGATGC AATATTCAGACTGACTGACCCCCTGCCTTATTTCACCAAAGACACGATGCATAGTCACCC CGGCCTTGTTTCTCCAATGGCCGTGATACACTAGTGATCATGTTCAGCCCTGCTTCCACC TGCATAGAATCTTTTCTTCTCAGACAGGGACAGTGCGGCCTCAACATCTCCTGGAGTCTA GAAGCTGTTTCCTTTCCCCTCCTTCCTCCTCTTGCTCTAGCCTTAATACTGGCCTTTTCC CTCCCTGCCCCAAGTGAAGACAGGGCACTCTGCGCCCACCACATGCACAGCTGTGCATGG AGACCTGCAGGTGCACGTGCTGGAACACGTNGTGGTTCCCCCCTGGCCCAGCCTCCTCTG CAGTGCCCCTCTCCCCTGCCCATCCTCCCCACGGAAGCATGTGCTGGTCACACTGGTTCT CCAGGNGGTCTGTGATGGGGCCCCTGNGGGTCAGCTTCTGTCCCTCTGCCTTCTCACCTC TNTGTTCCTTTCTTTTCATGTATCCATTCAGTTGATGTTTATTGAGCCACTACAGATGTC AGCACTGTGTTAGGTGCTGGGGGCCCTGCGTGGGAGAATAAAGTTNCTNCCTCAGGNACT CCCCATNCAGCTGGGAGACAGACACTAACTACACTGACCCCTGGCGTTGGAGGGGGGTCC TGNCTGGCTCCCGGCTTCACACTTCTTTGTGGCTCAGGGCTTCTGGNATACCTACCCCCT TCCACCATTATTCTACCAAGATGGGGG
Restriction Sites	NotI-NotI
ACCN	BC011578
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011578.1, AAH11578.1
RefSeq Size	1698 bp
RefSeq ORF	414 bp
Locus ID	80381
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)
Gene Summary	The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.