Porimin (TMEM123) (BD123550) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD123550, the custom clone sequence may differ by one or more nucleotides CCCGCCGCCCCGGCTGCGCACGCGACGCCCCCTCCAGGCCCCGCTCCTGCGCCCTATTTGGTCATTCGGG GGGCAAGCGGCGGGAGGGGAAACGTGCGCGGCCGAAGGGGAAGCGGAGCCGGCGCCGGCTGCGCAGAGGA GCCGCTCTCGCCGCCGCCACCTCGGCTGGGAGCCCACGAGGCTGCCGCATCCTGCCCTCGGAACAATGGG ACTCGGCGCGCGAGGTGCTTGGGCCGCGCTGCTCCTGGGGACGCTGCAGGTGCTAGCGCTGCTGGGGGCC GCCCATGAAAGCGCAGCCATGGCGGCATCTGCAAACATAGAGAATTCTGGGCTTCCACACAACTCCAGTG CTAACTCAACAGAGACTCTCCAACATGTGCCTTCTGACCATACAAATGAAACTTCCAACAGTACTGTGAA ACCACCAACTTCAGTTGCCTCAGACTCCAGTAATACAACGGTCACCACCATGAAACCTACAGCGGCATCT AATACAACAACACCAGGGATGGTCTCAACAAATATGACTTCTACCACCTTAAAGTCTACACCCAAAACAA CAAGTGTTTCACAGAACACATCTCAGATATCAACATCCACAATGACCGTAACCCACAATAGTTCAGTGAC ATCTGCTGCTTCATCAGTAACAATCACAACAACTATGCATTCTGAAGCAAAGAAAGGATCAAAATTTGAT ACTGGGAGCTTTGTTGGTGGTATTGTATTAACGCTGGGAGTTTTATCTATTCTTTACATTGGATGCAAAA TGTATTACTCAAGAAGAGGCATTCGGTATCGAACCATAGATGAACATGATGCCATCATTTAAGGAAATCC ATGGACCAAGGATGGAATACAGATTGATGCTGCCCTATCAATTAATTTTGGTTTATTAATAGTTTAAAAC AATATTCTCTTTTTGAAAATAGTATAAACAGGCCATGCATATAATGTACAGTGTATTACGTAAATATGTA AAGATTCTTCAAGGTAACAAGGGTTTGGGTTTTGAAATAAACATCTGGATCTTATAGACCGTTCATACAA TGGTTTTAGCAAGTTCATAGTAAGACAAACAAGTCCTATCTTTTTTTTTTTGGCTGGGGTGGGGGCATTG GTCACATATGACCAGTAATTGAAAGACGTCATCACTGAAAGACAGAATGCCATCTGGGCATACAAATAAG AAGTTTGTCACAGCACTCAGGATTTTGGGTATCTTTTGTAGCTCACATAAAGAACTTCAGTGCTTTTCAG AGCTGGATATATCTTAATTACTAATGCCACACAGAAATTATACAATCAAACTAGATCTGAAGCATAATTT AAGAAAAACATCAACATTTTTTGTGCTTTAAACTGTAGTAGTTGGTCTAGAAACAAAATACTCCAAGAAA AAGAAAATTTTCAAATAAAACCCAAAATAATAGCTTTGCTTAGCCCTGTTAGGGATCCATTGGAGCATTA AGGAGCACATATTTTTATTAACTTCTTTTGAGCTTTCAATGTTGATGTAATTTTTGTTCTCTGTGTAATT TAGGTAAACTGCAGTGTTTAACATAATAATGTTTTAAAGACTTAGTTGTCAGTATTAAATAATCCTGGCA TTATAGGGAAAAAACCTCCTAGAAGTTAGATTATTTGCTACTGTGAGAATATTGTCACCACTGGAAGTTA CTTTAGTTCATTTAATTTTAATTTTATATTTTGTGAATATTTTAAGAACTGTAGAGCTGCTTTCAATATC TAGAAATTTTTAATTGAGTGTAAACACACCTAACTTTAAGAAAAAGAACCGCTTGTATGATTTTCAAAAG AACATTTAGAATTCTATAGAGTCAAAACTATAGCGTAATGCTGTGTTTATTAAGCCAGGGATTGTGGGAC TTCCCCCAGGCAACTAAGCCTGCAGGATGAAAATGCTATATTTTCTTTCATGCACTGTCGATATTACTCA GATTTGGGGAAATGACATTTTTATACTAAAACAAACACCAAAATATTTTAGAATAAATTCTTAGAAAGTT TTGAGAGGAATTTTTAGAGAGGACATTTCCTCCTTCCCGATTTGGATATTCCCTCAAATCCCTCCTCTTA CTCCATGCTGAAGGAGAAGTACTCTCAGATGCATTATGTTAATGGAGAGAAAAAGCACAGTATTGTAGAG ACACCAATATTAGCTAATGTATTTTGGAGTGTTTTCCATTTTACAGTTTATATTCCAGCACTCAAAACTC AGGGTCAAGTTTTAACAAAAGAGGTATGTAGTCACAGTAAATACTAAGATGGCATTTCTATCTCAGAGGG CCAAAGTGAATCACACCAGTTTCTGAAGGTCCTAAAAATAGCTCAGATGTCCTAATGAACATGCACCTAC ATTTAATAGGAGTACAATAAAACTGTTGTCAGCTTTTGTTTTACAGAGAACGCTAGATATTAAGAATTTT GAAATGGATCATTTCTACTTGCTGTGCATTTTAACCAATAATCTGATGAATATAGAAAAAAATGATCCAA AATATGGATATGATTGGATGTATGTAACACATACATGGAGTATGGAGGAAATTTTCTGAAAAATACATTT AGATTAGTTTAGTTTGAAGGAGAGGTGGGCTGATGGCTGAGTTGTATGTTACTAACTTGGCCCTGACTGG TTGTGCAACCATTGCTTCATTTCTTTGCAAAATGTAGTTAAGATATACTTTATTCTAATGAAGGCCTTTT AAATTTGTCCACTGCATTCTTGGTATTTCACTACTTCAAGTCAGTCAGAACTTCGTAGACCGACCTGAAG TTTCTTTTTGAATACTTGTTTCTTTAGCACTTTGAAGATAGAAAAACCACTTTTTAAGTACTAAGTCATC ATTTGCCTTGAAAGTTTCCTCTGCATTGGGTTTGAAGTAGTTTAGTTATGTCTTTTTCTCTGTATGTAAG TAGTATAATTTGTTACTTTCAAATACCCGTACTTTGAATGTAGGTTTTTTTGTTGTTGTTATCTATAAAA ATTGAGGGAAATGGTTATGCAAAAAAATATTTTGCTTTGGACCATATTTCTTAAGCATAAAAAAAATGCT CAGTTTTGCTTGCATTCCTTGAGAATGTATTTATCTGAAGATCAAAACAAACAATCCAGATGTATAAGTA CTAGGCAGAAGCCAATTTTAAAATTTCCTTGAATAATCCATGAAAGGAATAATTCAAATACAGATAAACA GAGTTGGCAGTATATTATAGTGATAATTTTGTATTTTCACAAAAAAAAAGTTAAACTCTTCTTTTCTTTT TATTATAATGACCAGCTTTTGGTATTTCATTGTTACCAAGTTCTATTTTTAGAATAAAATTGTTCTCCTT CT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD123550 unedited NNNNGGTCGGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGCTCGGC TGGGAGCCCACGAGGCTGCCGCATCCTGCCCTCGGAACATGGGACTCGGCGCGCGAGGTG CTTGGGCCGCGCTGCTCCTGGGGACGCTGCAGGTGCTAGCGCTGCTGGGGGCCGCCCATG AAAGCGCAGCCATGGCGGCATCTGCAAACATAGAGAATTCTGGGCTTCCACACAACTCCA GTGCTAACTCAACAGAGACTCTCCAACATGTGCCTTCTGACCATACAAATGAAACTTCCA ACAGTACTGTGAAACCACCAACTTCAGTTGCCTCAGACTCCAGTAATACAACGGTCACCA CCATGAAACCTACAGCGGCATCTAATACAACAACACCAGGGATGGTCTCAACAAATATGA CTTCTACCACCTTAAAGTCTACACCCAAAACAACAAGTGTTTCACAGAACACATCTCAGA TATCAACATCCACAATGACCGTAACCCACAATAGTTCAGTGACATCTGCTGCTTCATCAG TAACAATCACAACAACTATGCATTCTGAAGCAAAGAAAGGATCAAAATTTGATACTGGGA GCTTTGTTGGTGGTATTGTATTAACGCTGGGAGTTTTATCTATTCTTTACATTGGATGCA AAATGTATTACTCAAGAAGAGGCATTCGGTATCGAACCATAGATGAACATGATGCCATCA TTTAAGGAAATCCATGGACCAAGGATGGAATACAGATTGATGCTGCCCTATCAATTAATT TTGGTTTATTAATAGTTTAAACATATTCTCTTTTGAAATAGTATAACAGGCCATGCTATA TGTACAGTGTATACGTAAAATGTAAAGATCTTCAGTAACCAGGNTTTGTGGN
Restriction Sites	NotI-NotI
ACCN	BD123550
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BD123550.1
RefSeq Size	3362 bp
RefSeq ORF	3362 bp
Locus ID	114908
Protein Families	Transmembrane
Gene Summary	This gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti-PORIMIN (pro-oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death. [provided by RefSeq, Jul 2008]

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