STARD5 (NM_030574) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MGC10327; StAR-related lipid transfer (START) domain containing 5; StAR-related lipid transfer protein 5; START domain containing 5
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_030574, the custom clone sequence may differ by one or more nucleotides ATGGACCCGGCGCTGGCAGCCCAGATGAGCGAGGCTGTGGCCGAGAAGATGCTCCAGTACCGGCGGGACA CAGCAGGCTGGAAGATTTGCCGGGAAGGCGTACCGAGGAGAAGGCATTGTATATGGGACACTAGAGGAGG TGTGGGACTGTGTGAAGCCAGCTGTTGGAGGCCTACGAGTGAAGTGGGATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_030574 unedited NAAGCGGTCAAATATTGTATACGACTCACTATAGGCGGCCGCGACATTCGCACGAGGNAG AACCTCCACTCCCTCCGCTGCCATGAAGCTCAGTTCTCCCAGAGATTTTGTGGACTTGGT GCTAGTCAAGAGATATGAGGATGGGACCATCAGTTCCAACGCCACCCATGTGGAGCATCC GTTATGTCCCCCGAAGCCAGGTTTTGTGAGAGGATTTAACCATCCTTGTGGTTGCTTCTG TGAACCTCTTCCAGGGGAACCCACCAAGACCAACCTGGTCACATTCTTCCATACCGACCT CAGCGGTTACCTCCCACAGAACGTGGTGGACTCCTTCTTCCCCCGCAGCATGACCCGGTT TTATGCCAACCTTCAGAAAGCAGTGAAGCAATTCCATGAGTAATGCTATCGTTACTTCTT GGCAAAGAACTCCCGTGACTCATCGAGGAGCTCCAGCTGTTGGGACACCAAGGAGCCTGG GAGCACGCAGAGGCCTGTGTTCACTCTTTGGAACAAGCTGATGGACTGCGCATCTCTGAG AATGCCAACCAGAGGCGGCAGCCCACCCTTCCTGCCTCCTGCCCCACTCAGGGTTGGCGT GTGATGAGCCATTCATGTGTTCCAAACTCCATCTGCCTGTTACCCAAACACGCCTCTCCT GGCAGGGTAGACCCAGGCCTCTAACCATCTGACAGAGACTCGGCCTGGACACCATGCGAT GCACTCTGGCACCAAGGCTTTATGTGCCCATCACTCTCAGAGACCACGTTTCCCTGACTG TCATAGAGAATCATCATCGCCACTGAAAACCAGGCCCTGTTGCCTTTTAAGCATGTACCG CTCCCTCAGTCCTGTGCTGCAGCCCCCCAATATATTTTTCTGATATAGACCTTGTATC
Restriction Sites	NotI-NotI
ACCN	NM_030574
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030574.1, NP_085051.1
RefSeq Size	1294 bp
RefSeq ORF	321 bp
Locus ID	80765
Gene Summary	Proteins containing a steroidogenic acute regulatory-related lipid transfer (START) domain are often involved in the trafficking of lipids and cholesterol between diverse intracellular membranes. This gene is a member of the StarD subfamily that encodes START-related lipid transfer proteins. The protein encoded by this gene is a cholesterol transporter and is also able to bind and transport other sterol-derived molecules related to the cholesterol/bile acid biosynthetic pathways such as 25-hydroxycholesterol. Its expression is upregulated during endoplasmic reticulum (ER) stress. The protein is thought to act as a cytosolic sterol transporter that moves cholesterol between intracellular membranes such as from the cytoplasm to the ER and from the ER to the Golgi apparatus. Alternative splicing of this gene produces multiple transcript variants. [provided by RefSeq, Jan 2016]

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