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Isoleucyl tRNA synthetase (IARS) (NM_002161) Human Untagged Clone
CAT＃: SC126589
IARS (untagged)-Human isoleucyl-tRNA synthetase (IARS), transcript variant short
CNY 8,792.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GRIDHH; IARS; ILERS; ILRS; IRS; PRO0785 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126589 sequence for NM_002161 edited (data generated by NextGen Sequencing)
ATGCTTCAACAAGTTCCAGAAAACATAAATTTTCCTGCTGAAGAAGAGAAAATCTTGGAG TTTTGGACTGAATTTAATTGTTTTCAGGAATGCTTAAAGCAATCAAAACATAAACCAAAA TTTACCTTCTATGATGGTCCTCCTTTTGCAACTGGACTGCCTCACTATGGACATATACTT GCGGGTACAATTAAAGATATAGTTACAAGATATGCTCACCAGAGTGGGTTTCATGTTGAC AGAAGATTTGGATGGGATTGCCATGGCTTACCTGTGGAATATGAAATTGATAAGACACTG GGAATCAGAGGACCAGAGGATGTGGCCAAAATGGGGATTACAGAGTATAACAATCAGTGC CGAGCAATTGTGATGAGATATTCTGCTGAGTGGAAGTCTACTGTTAGCAGACTTGGCCGA TGGATTGACTTTGACAATGACTATAAAACTCTGTATCCACAATTCATGGAATCAGTCTGG TGGGTCTTCAAACAACTCTATGATAAAGGCCTTGTTTATAGAGGTGTGAAAGTCATGCCC TTCTCTACGGCATGTAACACTCCACTTTCCAACTTCGAGTCACACCAGAATTATAAGGAT GTTCAAGATCCTTCAGTATTTGTAACTTTCCCTTTGGAAGAAGATGAAACTGTATCTTTA GTTGCTTGGACAACCACTCCCTGGACTCTACCTAGTAACCTTGCTGTGTGTGTTAATCCA GAAATGCAATATGTGAAAATTAAAGATGTTGCCAGAGGACGATTACTCATTTTAATGGAA GCCAGATTGTCAGCCCTCTATAAATTGGAGAGTGACTATGAGATCCTTGAAAGATTTCCT GGTGCCTATCTTAAAGGCAAGAAGTACAGGCCCCTGTTTGACTATTTCCTGAAGTGTAAA GAGAATGGCGCTTTCACTGTGCTTGTTGACAACTATGTGAAGGAAGAAGAAGGCACAGGG GTTGTCCACCAAGCTCCTTACTTCGGTGCTGAGGACTATCGGGTCTGTATGGACTTTAAC ATTATTCGGAAAGACTCACTCCCTGTTTGCCCTGTGGATGCTTCAGGCTGCTTCACAACG GAGGTGACAGATTTCGCAGGACAGTATGTGAAGGATGCTGACAAAAGTATCATCAGGACT TTGAAGGAACAAGGCCGACTTCTGGTTGCCACCACCTTCACTCACAGCTACCCTTTTTGC TGGAGATCAGACACTCCTCTAATTTACAAAGCAGTGCCCAGCTGGTTTGTGCGAGTGGAG AACATGGTGGACCAGCTCCTAAGGAACAATGACCTGTGCTACTGGGTCCCAGAGTTGGTA CGAGAAAAACGATTTGGAAATTGGCTGAAAGATGCACGTGACTGGACAATTTCCAGAAAC AGATACTGGGGCACCCCCATCCCACTGTGGGTCAGCGATGACTTTGAGGAGGTGGTATGC ATTGGGTCAGTGGCGGAACTTGAAGAACTGTCAGGAGCAAAGATCTCAGATCTCCACAGA GAGAGTGTTGACCACCTGACCATTCCTTCACGCTGTGGGAAGGGATCCTTGCACCGCATC TCTGAAGTGTTTGACTGTTGGTTTGAGAGTGGCAGCATGCCCTATGCTCAGGTTCATTAC CCGTTTGAAAACAAGAGGGAGTTTGAGGATGCTTTTCCTGCAGATTTCATTGCCGAGGGC ATCGACCAAACCAGAGGATGGTTTTATACCCTGCTGGTGCTGGCCACGGCCCTCTTTGGA CAACCGCCTTTCAAGAACGTAATTGTGAATGGGCTTGTCCTGGCAAGTGATGGCCAAAAA ATGAGCAAACGGAAAAAGAATTATCCAGATCCAGTTTCCATCATCCAGAAGTATGGTGCT GATGCCCTCAGATTATATCTGATTAACTCCCCTGTGGTGAGAGCAGAAAACCTCCGCTTT AAAGAAGAGGGTGTGCGGGACGTCCTTAAGGATGTACTGCTCCCATGGTACAATGCCTAT CGCTTCTTAATCCAGAACGTTCTGAGGCTCCAGAAGGAGGAAGAAATAGAATTTCTCTAC AATGAGAACACGGTTAGAGAAAGCCCCAACATTACAGACCGGTGGATCCTGTCCTTCATG CAGTCTCTCATTGGCTTCTTTGAGACTGAAATGGCAGCTTATAGGCTTTATACTGTGGTG CCTCGCCTGGTCAAGTTTGTAGATATTCTGACCAATTGGTATGTTAGAATGAACCGCAGA AGATTAAAGGGTGAAAATGGGATGGAGGATTGTGTCATGGCCCTAGAAACCTTGTTTAGT GTTCTGCTTTCTCTTTGCAGACTTATGGCTCCCTACACACCTTTTCTCACTGAATTGATG TACCAGAATCTAAAGGTGCTGATTGACCCTGTTTCTGTTCAGGACAAGGACACACTCAGC ATTCACTACCTCATGCTGCCCCGTGTTCGAGAAGAATTGATTGACAAGAAAACAGAGAGT GCAGTATCTCAGATGCAGTCTGTGATTGAACTTGGAAGAGTGATCAGAGACCGAAAAACT ATTCCCATAAAGTATCCTTTGAAAGAAATTGTGGTTATCCATCAAGATCCAGAAGCTCTT AAAGATATCAAGTCTTTGGAGAAGTATATCATTGAGGAACTCAATGTTCGAAAAGTTACA CTGTCTACAGATAAAAACAAGTATGGCATTCGGCTAAGGGCAGAACCAGATCACATGGTC CTGGGGAAGCGTCTGAAGGGAGCCTTTAAGGCAGTGATGACGTCCATCAAGCAGTTGAGC AGTGAGGAGCTGGAGCAGTTCCAGAAGACTGGGACCATTGTTGTGGAAGGCCATGAATTG CACGATGAAGACATCCGCCTCATGTACACCTTTGATCAGGCCACAGGTGGGACTGCGCAA TTTGAAGCACACTCAGATGCTCAGGCTTTGGTCCTCTTAGATGTCACTCCTGACCAGTCA ATGGTAGATGAAGGAATGGCTCGGGAAGTCATCAATCGCATACAGAAACTTCGCAAAAAG TGCAATCTGGTTCCAACTGATGAAATCACAGTGTACTATAAAGCAAAGTCTGAAGGAACA TATCTGAATAGTGTTATTGAAAGCCACACAGAGTTCATATTTACCACCATAAAGGCTCCC TTGAAACCATATCCAGTTTCTCCATCGGATAAAGTCCTTATTCAAGAAAAAACACAGTTG AAGGGATCTGAACTGGAAATTACACTCACCAGAGGATCTTCCCTTCCTGGTCCTGCTTGT GCATATGTCAATCTTAACATTTGTGCAAATGGCAGTGAACAAGGTGGAGTATTGCTCCTG GAAAATCCAAAAGGTGACAATAGGTTGGACCTTTTAAAGCTGAAGAGTGTTGTCACTAGC ATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCTTCCATGATGAAACAGAAATACAAAAC CAAACTGACTTACTGAGTCTTAGTGGAAAAACACTTTGTGTGACTGCAGGATCGGCTCCC TCTCTGATCAACAGTTCTAGTACTCTTCTTTGTCAGTATATCAACCTACAGCTCCTGAAT GCAAAGCCACAAGAGTGTTTAATGGGGACAGTGGGCACTCTCCTGCTTGAAAACCCACTT GGGCAGAATGGACTCACCCACCAAGGTCTTCTGTATGAAGCAGCCAAGGTGTTTGGCCTT CGGAGCAGGAAGCTAAAGCTGTTTCTGAATGAGACCCAAACGCAGGAAATTACAGAAGAC ATCCCCGTGAAGACTTTGAATATGAAGACTGTGTATGTTTCTGTGTTACCAACAACAGCA GACTTCTAG Clone variation with respect to NM_002161.4 >OriGene 5' read for NM_002161 unedited
CCCACTTNNNNNNNNNTNNNNNNNNNNNNGGTTCAAATTTGTATACGACTCACTATAGGC GGCCGCGAATTCGCACCAGGCCGGNAGAGTTGCTTTTAGGCTTGCTGGCCCGCGGGGCTG TCCAGGCACGCGAGGCCCCTCAGCAACAAAATGCTTCAACAAGTTCCAGAAAACATAAAT TTTCCTGCTGAAGAAGAGAAAATCTTGGAGTTTTGGACTGAATTTAATTGTTTTCAGGAA TGCTTAAAGCAATCAAAACATAAACCAAAATTTACCTTCTATGATGGTCCTCCTTTTGCA ACTGGACTGCCTCACTATGGACATATACTTGCGGGTACAATTAAAGATATAGTTACAAGA TATGCTCACCAGAGTGGGTTTCATGTTGACAGAAGATTTGGATGGGATTGCCATGGCTTA CCTGTGGAATATGAAATTGATAAGACACTGGGAATCAGAGGACCAGAGGATGTGGCCAAA ATGGGGATTACAGAGTATAACAATCAGTGCCGAGCAATTGTGATGAGATATTCTGCTGAG TGGAAGTCTACTGTTAGCAGACTTGGCCGATGGATTGACTTTGACAATGACTATAANACT CTGTATCCACAATTCATGGAATCAGTCTGGTGGGTCTTCAAACAACTCTATGATAAAGGG CCTTGTTTATAGAGGTGTGAAAGTCATGCCCTTCTCTACNGCATGTNACACTTCACTTTN CAACTTCGAGTCACACCCAGAATATAAGGATGTTCAAGATTCTTCAGTATTTGTAACCTT CCCCTTTGAAAGAGAAGAAACTGTATCTTTAGGTGCCTGGACAACCACTCCCTGGACTCT ACCTAGAACCCTTGTTGGGGGGGTAATCCACAAATGCCATATTGGAAAATTAAAGAGGTG CCCGAGGACGATTACTCATTTTATGGGAGCCCAATGGTCAGCCTTCTTTAATTTGGGAAA TGATCTTGAAATCCTTGAAGATTTNCTGGGGCCCTATTTAAGGCCAAAATCCAGGCCCCT TGTTTA >OriGene 3' read for NM_002161 unedited
TCTGTACGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTGAGTTTGTTTAT TGTTCTGATTTACTGAAACTTAAAATATTCCATCAAATCTATCCAGGAAAATCCAGGTGG CAGAAATATATAATATGTCCATTTCATCAAGAGGTCTCAAATAAATTTTAAAAGGCCAGA AAATGATATATATACTATGCCATTTAAATCACTTCTATCTTCTGTACTTAAGAACTCAAG TATAGAAATAAACTGTGGGCTGAAGTAACATTGTAACCTGCTCCCAACATGACTGCATAG GTGTCTAAGGTTAAGTGTGAAGATTACTGTGAGGTCTCAAGTTACTTGACTAATCAATCC CATTTGAATTTCAATCCAAGCAGCATATTTTACACACACCTGAAGGAAATATCTTCAGTG TGTTCATGTGCGTGTCTATGTGCATGTATGTGTAGGGGATAGGTGTAATTAGGGAAGGGC CTGCGCCAACACGTTGATCACCCCATGCTACAACCCCTCTGCCGCCGCCTACTCTTGACC CCGCGCTCCCTCTCTGCTCCAAGCCCCACTCCCCCGTACCACCCGCCTCCTCCCCGCCCC CCCCCTCGTCTCTCTCCACCTCCCACCCTCTCTCGGCTCCTCTCTCTTTTCCTCCACTTC CTCCCCTACGCCTCCCTACCCTCCCTCCCCCTCACTCTTTTCACTGCCCGCACCTACTCA CACCCCCCCCATCCACCCTCCGCCCCACCCGACTCGTCCCTTCCCTNCACCTACTCATCC ACTCTTGTTTCTACCTCCCCTCCCCCCACCGCTCCTCTCCCCTCCCCCCCTCCCCTTCCT GACTAGTTGTTTTCTGTTCTTTTTTATCACGCCCACGTCCCTCAATCCGGTTTTGAATCT TCTTCTCCCACCGCCTCCCCCTACCTACGTGCCACTCGACCAACCCCCGTCCCCCCTCGC CCGCCCATCCTCCTACCCGTCTCCCCCGAATCCTTTCCTCTCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002161 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002161.2, NP_002152.1 |
| RefSeq Size | 4335 bp |
| RefSeq ORF | 3789 bp |
| Locus ID | 3376 |
| UniProt ID | P41252 |
| Domains | tRNA-synt_1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis |
| Gene Summary | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (1) and variant 2 encode the functional protein. |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212911 | IARS (Myc-DDK-tagged)-Human isoleucyl-tRNA synthetase (IARS), transcript variant short |
CNY 8,784.00 |
|
| RC212911L3 | Lenti ORF clone of Human isoleucyl-tRNA synthetase (IARS), transcript variant short, Myc-DDK-tagged |
CNY 14,540.00 |
|
| RC212911L4 | Lenti ORF clone of Human isoleucyl-tRNA synthetase (IARS), transcript variant short, mGFP tagged |
CNY 14,540.00 |
|
| RG212911 | IARS (tGFP-tagged) - Human isoleucyl-tRNA synthetase (IARS), transcript variant short |
CNY 13,870.00 |
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