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ASAH1 (NM_177924) Human Untagged Clone
CAT＃: SC126563
ASAH1 (untagged)-Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AC; ACDase; ASAH; PHP; PHP32; SMAPME |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126563 sequence for NM_177924 edited (data generated by NextGen Sequencing)
ATGCCGGGCCGGAGTTGCGTCGCCTTAGTCCTCCTGGCTGCCGCCGTCAGCTGTGCCGTC GCGCAGCACGCGCCGCCGTGGACAGAGGACTGCAGAAAATCAACCTATCCTCCTTCAGGA CCAACGTACAGAGGTGCAGTTCCATGGTACACCATAAATCTTGACTTACCACCCTACAAA AGATGGCATGAATTGATGCTTGACAAGGCACCAATGCTAAAGGTTATAGTGAATTCTCTG AAGAATATGATAAATACATTCGTGCCAAGTGGAAAAGTTATGCAGGTGGTGGATGAAAAA TTGCCTGGCCTACTTGGCAACTTTCCTGGCCCTTTTGAAGAGGAAATGAAGGGTATTGCC GCTGTTACTGATATACCTTTAGGAGAGATTATTTCATTCAATATTTTTTATGAATTATTT ACCATTTGTACTTCAATAGTAGCAGAAGACAAAAAAGGTCATCTAATACATGGGAGAAAC ATGGATTTTGGAGTATTTCTTGGGTGGAACATAAATAATGATACCTGGGTCATAACTGAG CAACTAAAACCTTTAACAGTGAATTTGGATTTCCAAAGAAACAACAAAACTGTCTTCAAG GCTTCAAGCTTTGCTGGCTATGTGGGCATGTTAACAGGATTCAAACCAGGACTGTTCAGT CTTACACTGAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATTCTAGAATGGATT CTGGGAAAGAAAGATGCCATGTGGATAGGGTTCCTCACTAGAACAGTTCTGGAAAATAGC ACAAGTTATGAAGAAGCCAAGAATTTATTGACCAAGACCAAGATATTGGCCCCAGCCTAC TTTATCCTGGGAGGCAACCAGTCTGGGGAAGGTTGTGTGATTACACGAGACAGAAAGGAA TCATTGGATGTATATGAACTCGATGCTAAGCAGGGTAGATGGTATGTGGTACAAACAAAT TATGACCGTTGGAAACATCCCTTCTTCCTTGATGATCGCAGAACGCCTGCAAAGATGTGT CTGAACCGCACCAGCCAAGAGAATATCTCATTTGAAACCATGTATGATGTCCTGTCAACA AAACCTGTCCTCAACAAGCTGACCGTATACACAACCTTGATAGATGTTACCAAAGGTCAA TTCGAAACTTACCTGCGGGACTGCCCTGACCCTTGTATAGGTTGGTGA Clone variation with respect to NM_177924.3 214 g=>a;277 a=>g;737 t=>c >OriGene 5' read for NM_177924 unedited
CAGTAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGGGGAGT GGCGTTGGCTGCTAGAGCGATGCCGGGCCGGAGTTGCGTCGCCTTAGTCCTCCTGGCTGC CGCCGTCAGCTGTGCCGTCGCGCAGCACGCGCCGCCGTGGACAGAGGACTGCAGAAAATC AACCTATCCTCCTTCAGGACCAACGTACAGAGGTGCAGTTCCATGGTACACCATAAATCT TGACTTACCACCCTACAAAAGATGGCATGAATTGATGCTTGACAAGGCACCAATGCTAAA GGTTATAGTGAATTCTCTGAAGAATATGATAAATACATTCGTGCCAAGTGGAAAAGTTAT GCAGGTGGTGGATGAAAAATTGCCTGGCCTACTTGGCAACTTTCCTGGCCCTTTTGAAGA GGAAATGAAGGGTATTGCCGCTGTTACTGATATACCTTTAGGAGAGATTATTTCATTCAA TATTTTTTATGAATTATTTACCATTTGTACTTCAATAGTAGCAGAAGACAAAAAAGGTCA TCTAATACATGGGAGAAACATGGATTTTGGAGTATTTCTTGGGTGGAACATAAATAATGA TACCTGNGTCATAACTGAGCAACTAAAACCTTTAACAGTGAATTTGGATTTCCAAAGAAA CAACAAAACTGTCTTCAAGGCTTCAAGCTNTGCTGGCTATGTGGGCATGTTAACAGGATT CANACCAGGACTGTTCAGTCTTACACTGAATGAACGTTTCAGTATAAATGGTGGTTATCT GGGTATTCTAGAATGGATTCTGGGGAAGAAAGATGCCATGTGGATAGGGTTCCTCACTAC ACAGTTCTGGAAATAGCACAGTTATGAAGAGCCAAGAATTATTGACCAAGACAAGATATT GGCCCAGCCTACTTATCCTGTGAGCACCAGTCTGGGAAGGNTGTGTGATACCGAGACGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_177924 |
| Insert Size | 2260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177924.1, NP_808592.1 |
| RefSeq Size | 2341 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 427 |
| UniProt ID | Q13510 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome, Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 2. The resulting isoform (a) includes a predicted signal peptide, is shorter and has a distinct N-terminus compared to isoform b. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202135 | ASAH1 (Myc-DDK-tagged)-Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1 |
CNY 3,656.00 |
|
| RC202135L1 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202135L2 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RC202135L3 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202135L4 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG202135 | ASAH1 (tGFP-tagged) - Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1 |
CNY 5,256.00 |
