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MEST (NM_177524) Human Untagged Clone
CAT＃: SC126510
MEST (untagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 2
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PEG1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126510 sequence for NM_177524 edited (data generated by NextGen Sequencing)
ATGAGGGAGTGGTGGGTCCAGGTGGGGCTGCTGGCCGTGCCCCTGCTTGCTGCGTACCTG CACATCCCACCCCCTCAGCTCTCCCCTGCCCTTCACTCATGGAAGTCTTCAGGCAAGTTT TTCACTTACAAGGGACTGCGTATCTTCTACCAAGACTCTGTGGGTGTGGTTGGAAGTCCA GAGATAGTTGTGCTTTTACACGGTTTTCCAACATCCAGCTACGACTGGTACAAGATTTGG GAAGGTCTGACCTTGAGGTTTCATCGGGTGATTGCCCTTGATTTCTTAGGCTTTGGCTTC AGTGACAAACCGAGACCACATCACTATTCCATATTTGAGCAGGCCAGCATCGTGGAAGCG CTTTTGCGGCATCTGGGGCTCCAGAACCGCAGGATCAACCTTCTTTCTCATGACTATGGA GATATTGTTGCTCAGGAGCTTCTCTACAGGTACAAGCAGAATCGATCTGGTCGGCTTACC ATAAAGAGTCTCTGTCTGTCAAATGGAGGTATCTTTCCTGAGACTCACCGTCCACTCCTT CTCCAAAAGCTACTCAAAGATGGAGGTGTGCTGTCACCCATCCTCACACGACTGATGAAC TTCTTTGTATTCTCTCGAGGTCTCACCCCAGTCTTTGGGCCGTATACTCGGCCCTCTGAG AGTGAGCTGTGGGACATGTGGGCAGGGATCCGCAACAATGACGGGAACTTAGTCATTGAC AGTCTCTTACAGTACATCAATCAGAGGAAGAAGTTCAGAAGGCGCTGGGTGGGAGCTCTT GCCTCTGTAACTATCCCCATTCATTTTATCTATGGGCCATTGGATCCTGTAAATCCCTAT CCAGAGTTTTTGGAGCTGTACAGGAAAACGCTGCCGCGGTCCACAGTGTCGATTCTGGAT GACCACATTAGCCACTATCCACAGCTAGAGGATCCCATGGGCTTCTTGAATGCATATATG GGCTTCATCAACTCCTTCTGA Clone variation with respect to NM_177524.1 >OriGene 5' read for NM_177524 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAACCGGTTCTCCGAA ACATGGAGTCCTGTAGGCAAGGTCTTACCTGAATCAGGATGAGGGAGTGGTGGGTCCAGG TGGGGCTGCTGGCCGTGCCCCTGCTTGCTGCGTACCTGCACATCCCACCCCCTCAGCTCT CCCCTGCCCTTCACTCATGGAAGTCTTCAGGCAAGTTTTTCACTTACAAGGGACTGCGTA TCTTCTACCAAGACTCTGTGGGTGTGGTTGGAAGTCCAGAGATAGTTGTGCTTTTACACG GTTTTCCAACATCCAGCTACGACTGGTACAAGATTTGGGAAGGTCTGACCTTGAGGTTTC ATCGGGTGATTGCCCTTGATTTCTTAGGCTTTGGCTTCAGTGACAAACCGAGACCACATC ACTATTCCATATTTGAGCAGGCCAGCATCGTGGAAGCGCTTTTGCGGCATCTGGGGCTCC AGAACCGCAGGATCAACCTTCTTTCTCATGACTATGGAGATATTGTTGCTCAGGAGCTTC TCTACAGGTACAAGCAGAATCGATCTGGTCGGCTTACCATAAAGAGTCTCTGTCTGTCAA ATGGAGGTATCTTTCCTGAGACTCACCGTCCACTCCTTCTCCAAAAGCTACTNCAAAGAT GGAGGTGTGCTGTCACCCATCCTCACACGACTGATGAACTTCTTTGTATTCTCTCGAGGT CTCACCCCAGTCTTTGGGCCGTATACTCGGCCCTCTGAGAGTGAGCTGTGGGACATGTGG GCANGGATCCGCAACATGACGGGAACTTAGTCATNGACAGTCTCTNACAGTACATCAATC AGAAGGAAGAAGNTCAGAGGGCGCTGGGTGGGAAGCTCTGCCTCTGTAACTATCCCATTC ATTTANTATGGGCCATTGGATCTGTAAATCCTATCCGNATN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_177524 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177524.1, NP_803490.1 |
| RefSeq Size | 2455 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 4232 |
| UniProt ID | Q5EB52 |
| Protein Families | Protease, Transmembrane |
| Gene Summary | This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (b), which has a shorter N-terminus compared to isoform a. This isoform (b) is biallelically expressed. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212633 | MEST (Myc-DDK-tagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 2 |
CNY 2,400.00 |
|
| RC212633L3 | Lenti-ORF clone of MEST (Myc-DDK-tagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 2 |
CNY 5,890.00 |
|
| RC212633L4 | Lenti-ORF clone of MEST (mGFP-tagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 2 |
CNY 5,890.00 |
|
| RG212633 | MEST (tGFP-tagged) - Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 2 |
CNY 4,000.00 |
