Cytokeratin 8 (KRT8) (BC008200) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CARD2; CK8; CYK8; cytokeratin 8; K2C8; K8; keratin, type II cytoskeletal 8; Keratin 8; KO
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC008200 edited ATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATAGACAAGGTACGGTTCCTGGAGCAG CAGAACAAGATGCTGGAGACCAAGTGGAGCCTCCTGCAGCAGCAGAAGACGGCTCGAAGC AACATGGACAACATGTTCGAGAGCTACATCAACAACCTTAGGCGGCAGCTGGAGACTCTG GGCCAGGAGAAGCTGAAGCTGGAGGCGGAGCTTGGCAACATGCAGGGGCTGGTGGAGGAC TTCAAGAACAAGTATGAGGATGAGATCAATAAGCGTACAGAGATGGAGAACGAATTTGTC CTCATCAAGAAGGATGTGGATGAAGCTTACATGAACAAGGTAGAGCTGGAGTCTCGCCTG GAAGGGCTGACCGACGAGATCAACTTCCTCAGGCAGCTATATGAAGAGGAGATCCGGGAG CTGCAGTCCCAGATCTCGGACACATCTGTGGTGCTGTCCATGGACAACAGCCGCTCCCTG GACATGGACAGCATCATTGCTGAGGTCAAGGCACAGTACGAGGATATTGCCAACCGCAGC CGGGCTGAGGCTGAGAGCATGTACCAGATCAAGTATGAGGAGCTGCAGAGCCTGGCTGGG AAGCACGGGGATGACCTGCGGCGCACAAAGACTGAGATCTCTGAGATGAACCGGAACATC AGCCGGCTCCAGGCTGAGATTGAGGGCCTCAAAGGCCAGAGGGCTTCCCTGGAGGCCGCC ATTGCAGATGCCGAGCAGCGTGGAGAGCTGGCCATTAAGGATGCCAACGCCAAGTTGTCC GAGCTGGAGGCCGCCCTGCAGCGGGCCAAGCAGGACATGGCGCGGCAGCTGCGTGAGTAC CAGGAGCTGATGAACGTCAAGCTGGCCCTGGACATCGAGATCGCCACCTACAGGAAGCTG CTGGAGGGCGAGGAGAGCCGGCTGGAGTCTGGGATGCAGAACATGAGTATTCATACGAAG ACCACCAGCGGCTATGCAGGTGGTCTGAGCTCGGCCTATGGGGGCCTCACAAGCCCCGGC CTCAGCTACAGCCTGGGCTCCAGCTTTGGCTCTGGCGCGGGCTCCAGCTCCTTCAGCCGC ACCAGCTCCTCCAGGGCCGTGGTTGTGAAGAAGATCGAGACACGTGATGGGAAGCTGGTG TCTGAGTCCTCTGACGTCCTGCCCAAGTGAACAGCTGCGGCAGCCCCTCCCAGCCTACCC CTCCTGCGCTGCCCCAGAGCCTGGGAAGGAGGCCGCTATGCAGGGTAGCACTGGGAACAG GAGACCCACCTGAGGCTCAGCCCTAGCCCTCAGCCCACCTGGGGAGTTTACTACCTGGGG ACCCCCCTTGCCCATGCCTCCAGCTACAAAACAATTCAATTGCTTTTTTTTTTTGGTCCA AAATAAAACCTCAGCTAGCTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008200 unedited AGATTTTGTAAACGACTTATATAGGCGGACCGCGAATCAGATCTGGTACCGGTCCGGAAT TCCCGGGATATCGTCGACCCACGCGTCCGCTGGAGACCAAGTGGAGCCTCCTGCAGCAGC AGAAGACGGCTCGAAGCAACATGGACAACATGTTCGAGAGCTACATCAACAACCTTAGGC GGCAGCTGGAGACTCTGGGCCAGGAGAAGCTGAAGCTGGAGGCGGAGCTTGGCAACATGC AGGGGCTGGTGGAGGACTTCAAGAACAAGTATGAGGATGAGATCAATAAGCGTACAGAGA TGGAGAACGAATTTGTCCTCATCAAGAAGGATGTGGATGAAGCTTACATGAACAAGGTAG AGCTGGAGTCTCGCCTGGAAGGGCTGACCGACGAGATCAACTTCCTCAGGCAGCTATATG AAGAGGAGATCCGGGAGCTGCAGTCCCAGATCTCGGACACATCTGTGGTGCTGTCCATGG ACAACAGCCGCTCCCTGGACATGGACAGCATCATTGCTGAGGTCAAGGCACAGTACGAGG ATATTGCCAACCGCAGCCGGGCTGAGGCTGAGAGCATGTACCAGATCAAGTATGANGAGC TGCAGAGCCTGGCTGGGAAGCACGGNGATGACCCTGCGCGCACAAAGACTGAGATCTCTG AGATGAACCGGACATCAGCCCGCTCCAGGCTGAGATTGAGGGCCCTCAAGGCCAGAGGGC TTCCTGGAGGCCGCATTGCAAATGCCCAGCAACGTGGAAAGCTGGGCACTAAAGATGCCA ACCCCAATTGTCCGAGCTTGAGGCCCCCCTGCAGCGGGGCAAACAGAAAATGCCGCGGCA ACTGCCTGAATACCCAGAGCTGATGAACTTAAACTGGGCCTGGACATTAAAATCCGCACC TACAGAAACTTCTTGAAGCCAGAGAAAACCGCTTGAAATTGGAATCGCCAAAT
Restriction Sites	Please inquire
ACCN	BC008200
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008200.1, AAH08200.1
RefSeq Size	1437 bp
Locus ID	3856
Protein Families	Druggable Genome
Gene Summary	This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

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