FBXO22 (BC039024) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	F-box only protein 22; F-box protein 22; FBX22; FISTC1; FIST domain containing 1; FLJ13986; MGC31799
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC039024 edited CCACGCGTCCGAATCGGGTTCCTCCGAGCCGCCGTAGGACTGGTTCCGGCGGGCTGGTGA GGAATGGAGCCGGTAGGCTGCTGCGGCGAGTGCCGCGGCTCCTCCGTAGACCCGCGGAGC ACCTTCGTGTTGAGTAACCTGGCGGAGGTGGTGGAGCGTGTGCTCACCTTCCTGCCCGCC AAGGCGTTGCTGCGGGTGGCCTGAATGTTCGCATCTTACCACATACAGTTCTTTACATGG CTGATTCAGAAACTTTCATTAGTCTGGAAGAGTGTCGTGGCCATAAGAGAGCAAGGAAAA GAACTAGTATGGAAACAGCACTTGCCCTTGAGAAGCTATTCCCCAAACAATGCCAAGTCC TTGGGATTGTGACCCCAGGAATTGTAGTGACTCCAATGGGATCAGGTAGCAATCGACCTC AGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTATTATTCCCTCAAATTGAAGGAATAA AAATACAACCCTTTCATTTTATTAAGGATCCAAAGAATTTAACATTAGAAAGACATCAAC TCACTGAAGTAGGTCTTTTAGATAACCCTGAACTTCGTGTGGTCCTTGTCTTTGGTTATA ATTGCTGTAAGGTGGGAGCCAGTAATTATCTGCAGCAAGTAGTCAGCACTTTCAGTGATA TGAATATCATCTTGGCTGGAGGCCAGGTGGACAACCTGTCATCACTGACTTCTGAAAAGA ACCCTCTGGATATTGATGCCTCGGGTGTGGTTGGACTGTCATTTAGTGGACACCGAATCC AGAGTGCCACTGTGCTCCTCAACGAGGACGTCAGTGATGAGAAGACTGCTGAGGCTGCGA TGCAGCGCCTCAAAGCGGCCAACATTCCAGAGCATAACACCATTGGCTTCATGTTTGCAT GCGTTGGCAGGGGCTTTCAGTATTACAGAGCCAAGGGGAATGTTGAGGCTGATGCATTTA GAAAGTTTTTTCCTAGTGTTCCCTTATTCGGCTTCTTTGGAAATGGAGAAATTGGATGTG ATCGGATAGTCACTGGGAACTTTATATTGAGGAAATGTAATGAGGTAAAAGATGATGATC TGTTTCATAGCTATACAACAATAATGGCACTCATACATCTGGGGTCATCTAAATAATAAT TAAAGTGGCTTTCATAATATGTAACTTTTGGGTTCTGCCTTTTTCAGAAAATGGAAACTT GGGCCATGTGTATTTCAAACAAAAATAACTTTAGATATATCTTTTTTGTAGCTTTGATTG ATGCTCTAAGATCACATGAGGGTAGTATTTAATATATTAGATGAAGGACAACTTTGGACA TAACACTGACTAGGAGTTGAGAGCTTTTGCATCAGGCAGAAGCAAACTGATTATAGTTGT GTTGCACCAGATCATGTAGCTGCTGTGTAACATGACCTTAAATAGTCTTCCTGCATAGGA AGAGCAAAAGGGTATTCATCAATAGGATATAGATTTAAGACATTCCCTGACTACCCCTTG CGTTGTTAGGTGATGTCTTTTAGCAGAATCATGAAGACCTTTTTTCTCCCTTAATAAAGG AGAAAAATATACTGATGGCTGGAGAAATTTTTCTCTGCCTTTCAGTTTTATGAATTTTTT CAGAAGTAACAATATTATTATTGACTTTTTACTTATTTGATAAAAATTAAAGAACTATTT TTGTTTTGGTCAGATAAATTGACAAGACTAATCAGTATTTTATTATAAGTAAAAGATTTT TCTTCTTTCCTTAAAAATATTTTTTTTTCACCTAGGTCTAAATAGCTAACTAACTGGTAG ACCAGAGTATTACATCATCTTATTTTGGTTTTATACCAATAAAACATAGCGTGGAACTCA TTCAGGTAATGTTTTGCATTTCATTGCTTTTGGATGAACAAAGGAAGTAAACTAATCCTT TATAAATGAAAACCCAGAATAGTTGGTATGTCAGCTAGTCATTCCTGTCATATTCCCAGT AGAATGATTTTCAAGTTTGAATTTCTGTACAAATATCTAAATAAGAGATGTGCAGAGAGC ACCAATTTTCCTTCAATATCCATTCTTTACTTTTCACATAATGATAGAACCTTTGATTTT TCAAGTGGGTATGCCTCCTAGAATAAAGACTACATTTCCCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC039024 unedited GAGGTTTTGATTTGTATACGACTCACTATAGGGCGGCCGCGATTCANATCTGGTACCGGT CCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGAATCGGGTTCCTCCGAGCCGCCGT AGGACTGGTTCCGGCGGGCTGGTGAGGAATGGAGCCGGTAGGCTGCTGCGGCGAGTGCCG CGGCTCCTCCGTAGACCCGCGGAGCACCTTCGTGTTGAGTAACCTGGCGGAGGTGGTGGA GCGTGTGCTCACCTTCCTGCCCGCCAAGGCGTTGCTGCGGGTGGCCTGAATGTTCGCATC TTACCACATACAGTTCTTTACATGGCTGATTCAGAAACTTTCATTAGTCTGGAAGAGTGT CGTGGCCATAAGAGAGCAAGGAAAAGAACTAGTATGGAAACAGCACTTGCCCTTGAGAAG CTATTCCCCAAACAATGCCAAGTCCTTGGGATTGTGACCCCAGGAATTGTAGTGACTCCA ATGGGATCAGGTAGCAATCGACCTCAGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTA TTATTCCCTCAAATTGAAGGAATAAAAATACAACCCTTTCATTTTATTAAGGATCCAAAG AATTTAACATTAGAAAGACATCAACTCACTGAAGTAGGTCTTTTAGATAACCCTGAACTT CGTGTGGTCCTTGTCTTTGGTTATAATTGCTGNTAGGTGGGAGCCAGTAATTATCTGCAG CAAGTAGTCAGCACTTTCAGTGATATGAATATCATCTTGGCTGGGAGCCANNGTGGACAC CTGTCATCACTGACTTCTGAAAAGACCCTCTGGATATTGATGCCNNTCGGTGTGGTTGGA CTGTCATTTAGTGGACACCGATCCAGAGTGCCACTGTGCTNCTCACGN
Restriction Sites	NotI-NotI
ACCN	BC039024
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC039024.1, AAH39024.1
RefSeq Size	2188 bp
Locus ID	26263
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and, as a transcriptional target of the tumor protein p53, is thought to be involved in degradation of specific proteins in response to p53 induction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

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