PAK4 (NM_001014833) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_001014833 edited GGGGATTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAAGGA GGCGCGAGGGCGCGGAGTTCCAGGCCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAG CCGATGAGTAACCCGAAGCCCCTAGAGGAGTGGTCACCTGCCTGAGGGCACTTCTGTCCC ACCAGCATCAGACCAGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGC GGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGCGTGCACACGGGCTTCGACCAGC ACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTC GCCGGCCCAAGCCCCTCGTCGACCCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCA AGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGACG AGTTTGAGAACATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGC CCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAAAAGCCCCCTGGCCCCCGCTCACCAC AGCGGGAGCCACAGCGAGTATCCCATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGG ACCCAGGCGACCCCCGCTCCTACCTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGG GCATCGTGTGCATCGCCACCGTGCGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGG ACCTGCGCAAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACT ACCAGCACGAGAATGTGGTGGAGATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGG TGGTCATGGAGTTCCTGGAAGGAGGCGCCCTCACCGACATCGTCACCCACACCAGGATGA ACGAGGAGCAGATCGCGGCCGTGTGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACG CCCAGGGCGTCATCCACCGGGACATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCA GGGTGAAGCTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGA AGTCGCTGGTCGGCACGCCCTACTGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACG GGCCAGAGGTAGACATCTGGTCGCTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGC CCCCCTACTTCAACGAGCCACCCCTCAAAGCCATGAAGATGATTCGGGACAACCTGCCAC CCCGACTGAAGAACCTGCACAAGGTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGC TGGTGCGAGACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGG CCAAGGCAGGGCCGCCTGCCAGCATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGAG GCCCAGCGCCCTTCCCCTCAACCAAAGAGCCCCCCGGGTCACCCCCGCCCCACTGAGGCC AGTAGGGGGCCAGGCCTCCCACTCCTCCCAGCCCGGGAGATGCTCCGCGTGGCACCACCC TCCTTGCTGGGGGTAGATGAGACCCTACTACTGAACTCCAGTTTTGATCTCGTGACTTTT AGAAAAACACAGGGACTCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGAC AGGCCCTCCCCCATGTTCTTCTGTCTCCAGGAAGGGCAGCGGCCCTCCCATCACTGGAAG TCTGCAGTGGGGGTCGCTGGGGGTGGAGAGAACACTAAGAGGTGAACATGTATGAGTGTG TGCACGCGTGTGAGTGTGCATGTGTGTGTGTGTGCAAAGGTCCAGCCACCCCGTCCTCCA GCCCGCAAGGGGTGTCTGGCGCCTTGCCTGACACCCAGCCCCCTCTCCCCCTGAGCCATT GTGGGGGTCGATCATGAATGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTT CTGTGGCTGGATGGGGAGACAGGTCAGGGCCCCCCACCCTCTCCAGCCCCTGCAGCAAAT GACTACTGCACCTGGACAGCCTCCTCTTTTCTAGAAGTCTATTTATATTGTCATTTTATA ACACTCTAGCCCCTGCCCTTATTGGGGGACAGATGGTCCCTGTCCTGCGGGGTGGCCCTG GCAGAACCACTGCCTGAAGAACCAGGTTCCTGCCCGGTCAGCGCAGCCCCAGCCCGCCCA CCCCTGCCTCGAGTTAGTTTTACAATTAAAACATTGTCTTGTAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_001014833 unedited NNGGTAGACGTTTAGGATTTGTNATACGACTTACTATAGGCGGCCGCGTAATCAAATCTG GTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGTTCAACATGGCGGCGG GAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAAGGAGGCGCGAGGGCGCGGAGTTCC AGGCCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGGCCGCACCGAGTCCCCGG CACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCA CCGCGTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTG GCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGACCCCGCCTGCAT CACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGA TGGGGCCCTCACGCTGCTGCTGGACGAGTTTGAGAACATGTCGGTGACACGCTCCAACTC CCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGA GGAGCCGGCCACCACGGCCAGAGGGGGCCCAGGGAATGCAGGCAGCCGAGGCCGGTTCGC CGGTCACAGCGAGTCTTTTGGCGGCAATGGTGACAGGTTATGGNCGGGCCATATAAGAGG CCCAAGTCTTCCAGTGAGGGCTCACGGGGTTCCCCAGTAGTCCTCGCGGGATAACGCGTC CTCTTCGGGCCTGATTTCGGCACCCCCTTACTTGTTTGGTTTGGCCAGTGGGGCAAAACC GGCGCTTGTGCGGTCCTTAAAACC
Restriction Sites	Please inquire
ACCN	NM_001014833
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001014833.1, NP_001014833.1
RefSeq Size	2346 bp
RefSeq ORF	2346 bp
Locus ID	10298
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
Gene Summary	PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (6) lacks an in-frame segment in the coding region, as compared to variant 1. The encoded isoform (3) thus lacks an internal segment, as compared to isoform 1.

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