PLA2G12A (BC057844) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC057844 edited AGCGAGGCCCTGCCTGCCAGCCGGGAAGGAGGCGTGGTTATGGAGCTGGCGGCTGCAACC CCGGGGCCCGCGCCGCTGCCTAGCGCTTCCCGGGGGCTCTTTGGGGACGCGCCCCCCGCC GCGGCTCGGGGACCCGTAGAGCCCGGCGCTGCGCGGTTGGCCCTTCTCCCGCGCCCCGCG CTCACCCTCCTGCTCCTCCTCATGGCAGCTGTTATCAGGTGCCTGGAGCAGGCCTAGACC ACCGACTGGAGGGCCACCCTGAAGACCATCCGGAACGGCGTTCATAAGATAGACACGTAC CTGAACGCCGCCTTGGACCTCCTGGGAGGCGAGGACGGTCTCTGCCAGTATAAATGCAGT GACGGATCTAAGCCTTTCCCACGTTATGGTTATAAACCCTCCCCACCGAATGGATGTGGC TCTCCACTGTTTGGCGTTCATCTTAACATTGGTATCCCTTCCCTGACAAAGTGTTGCAAC TAACACGACAGGTGCTATGAGAACTGTGGCAAAAGCAAGAATGACTGTGATGAGAAATTC CAGTATTGCCTCTCCAAGATCTGCTGAGAAGTACAGAAAACACTAGGACTAACTTAGCAT GTTCAGGCATGTGAAACAACAATGGAGCTCTTGTTTGACAGTGTTATACATTTAGGTTGT AAACCATATCTGGACAGCCAACGAGCCACATGCAGGTGTCATTATGAAGAAAAAACCGAT ATTTAAAGGAGATGCTGACAGCTGGTGACAGATGAAGACGGAAGAACATAACCTTTGACA AATAACTAATGTTTTTACAACATAAAACTATCTGATTTTTGTGAAAAAAGAAAAACAAAC ATAGTTGGAGCTACCTGTACATCTCCCATATTGCAACACCTCCCTTTCCCAAAGATGTAC CCATTATCCTAAATTTAGTGTTTATTATTCCTGAGCATTTCTATTTCTGTGTAGGTATCC TTAAGCAATACATAGTATTTTTTTTGCCAGCTTTTAAAAATTATATCATTGGTTACATAC AGTGTAATTCTTCAACCTTTTTCTACTCAACCCTGTTTGTAAGCTTCACTCAAATTGACA TGTATACTGTATCTAGTTAGCTGATTTTCATTTCTGTTATGATTCCATAGCATGAAAATA AGTCACTTTCCCAATGGCTTACCATTATAGAGTGCTATGAACTTTTTGTATATGACTCTT GGTGTACGTATGTGAATTTCTCTAGAGCATATACTTAGGAATGGAATTGCTGGCTGCATT AGTCAGGTTTCTCTAGAGGAACAGAACTAATAGGATATATATATATATATATGTGTGTGT GTGTGTGTATATATATATGTGTGTGTGTGTGTATATATATGTGTGTATATATATATGTGT ATATATATATATAGTCCCACAATAGGCTGTCTGCATGCTCTATTAACCATCACACTGGCT GATATGGTTTGGCTCTGTGTTCCCACCCAAATCTCATCTTGAATAGTAATCCCATAATCC CCATGTGTCCTGGGAGGCACCTTGTGGGAGGTAATTGAATCATGGTTTCCTTCATGCTGT TCTTGTGATAGGAGTGAGTTCTCACGAGATCTGATGGTTTTATAAGCATCTGGCATTTCC CCTGCTGGCACTCATTCTCTCTCCTGCTGCCCTGTGAAGAGGTGCCTTTCACCATGATTT TAAGTTTCCTGAGGGCTCCCCAGCCATGTGGAACTGTGAGTCAATTAAACCTCTTTTCTT TATAAACAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC057844 unedited GTCATAATTTGTATACGACTCACTATAGGCGGCCGCATAACTTCGTATAGCATACATTAT ACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGCGAGGC CCTGCCTGCCAGCCGGGAAGGAGGCGTGGTTATGGAGCTGGCGGCTGCAACCCCGGGGCC CGCGCCGCTGCCTAGCGCTTCCCGGGGGCTCTTTGGGGACGCGCCCCCCGCCGCGGCTCG GGGACCCGTAGAGCCCGGCGCTGCGCGGTTGGCCCTTCTCCCGCGCCCCGCGCTCACCCT CCTGCTCCTCCTCATGGCAGCTGTTATCAGGTGCCTGGAGCAGGCCTAGACCACCGACTG GAGGGCCACCCTGAAGACCATCCGGAACGGCGTTCATAAGATAGACACGTACCTGAACGC CGCCTTGGACCTCCTGGGAGGCGAGGACGGTCTCTGCCAGTATAAATGCAGTGACGGATC TAAGCCTTTCCCACGTTATGGTTATAAACCCTCCCCACCGAATGGATGTGGCTCTCCACT GTTTGGCGTTCATCTTAACATTGGTATCCCTTCCCCTGACAAGTGTTGCAACTAACACGA CAGGTGCTATGAGAACTGTGGCAAAAGCAAGAATGACTGTGATGAGAAATTCCAGTATTG CCTCCTCCAGATCTGCTGAGAAGTACAGAAAACACTAGGACTAACTTTANCATGTTCAGG CATGTGAAACCAACATGGGAAGCTCTTGGTTGACAGTGTTATACATTTAAGTTGGTAAAC CATATCTGGGACAGCAACGAGCCACAGGCAGGTGTCCATTTTGAAGAAAAAACCGATATT AAAAGGAAATGCTGACAGCTGGTGACAGATGAAGACCGGAGAACATAACCCTTGACAAAT AACAAAGTTTTTACACATAAAACTTTTGATTTTTGTTGAAAAAGAAA
Restriction Sites	Please inquire
ACCN	BC057844
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057844.1, AAH57844.1
RefSeq Size	1762 bp
Locus ID	81579
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
Gene Summary	Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.