CPN1 (BC027897) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	anaphylatoxin inactivator; arginine carboxypeptidase; carboxypeptidase N, polypeptide 1, 50kD; carboxypeptidase N catalytic subunit; carboxypeptidase N polypeptide 1 50 kD; carboxypeptidase N small subunit; CPN; FLJ40792; kininas; OTTHUMP00000020269; SCPN
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC027897 edited TGCAAAAAACCTTTCAAAAGGCTTTCCTGGATTAGAGAAAGAAAGGGAGTGAGGGAGGAG AGATGAGTGGCTATTCCAGAACGACATAAAGAATTTCCAGCCTTGGACGGACAGCTGGGA ACGTCTTCCAATTTGGACTGGTGTTTACAAGCGGGAAGCTAGGTGGACCTTGGATTTTGG CGGGTGAAGAGGCTAGGTTGTTTAAGGAGGTGGGGCGCGTTTCAGTGGCTCTCTTTGAAA AAGCCCAGCAAGATGTCAGACCTGCTCTCAGTCTTCCTCCACCTCCTCCTTCTCTTCAAG TTGGTTGCCCCGGTGACCTTTCGCCACCACCGCTATGATGATCTTGTGCGGACGCTGTAC AAGGTGCAAAACGAATGCCCCGGCATCACGCGGGTCTACAGCATTGGGCGCAGCGTGGAG GGGAGACACCTCTACGTGCTGGAGTTCAGCGACCACCCTGGAATCCACGAGCCCTTGGAA CCAGAGGTCAAGTATGTGGGGAACATGCACGGCAACGAAGCGTTGGGCCGCGAGCTGATG CTGCAGCTGTCGGAGTTTCTGTGCGAGGAGTTCCGGAACAGGAACCAGCGCATCGTCCAG CTCATCCAGGACACGCGCATTCACATCCTGCCATCCATGAACCCCGACGGCTACGAGGTG GCTGCTGCCCAGGGCCCAAACAAGCCTGGGTATCTAGTTGGCAGGAACAATGCAAATGGA GTGGACCTGAACCGCAACTTCCCTGATCTCAATACCTATATCTACTATAACGAGAAGTAC GGAGGCCCCAACCACCACCTGCCCCTTCCAGACAACTGGAAAAGTCAGGTGGAACCCGAG ACCCGGGCGGTGATCCGGTGGATGCACTCCTTCAACTTTGTTCTTTCAGCCAATCTCCAC GGAGGGGCGGTGGTGGCCAATTACCCGTATGACAAGTCCTTTGAGCACCGGGTCCGAGGG GTCCGCCGCACCGCCAGCACCCCCACGCCTGACGACAAGCTCTTCCAGAAGCTGGCCAAG GTCTACTCCTATGCACATGGATGGATGTTCCAAGGTTGGAACTGCGGAGATTACTTCCCA GATGGCATCACCAATGGAGCTTCCTGGTATTCTCTCAGCAAGGGAATGCAAGACTTTAAT TATCTCCATACCAACTGCTTTGAGATCACGCTGGAACTGAGTTGCGACAAGTTTCCCCCC GAAGAGGAGTTACAGCGGGAGTGGCTGGGTAATCGGGAAGCCCTAATCCAGTTCCTGGAA CAGGTTCACCAGGGCATCAAGGGAATGGTGCTTGATGAGAATTACAATAATCTCGCCAAT GCTGTCATTTCTGTCAGTGGGATTAACCATGATGTCACTTCAGGTGACCATGGTGATTAC TTCCGGCTGCTGCTTCCAGGTATCTACACTGTTAGTGCCACAGCACCTGGGTATGACCCA GAGACAGTAACTGTGACCGTGGGTCCTGCGGAACCAACGTTGGTTAACTTCCACCTCAAA AGAAGCATCCCTCAAGTAAGCCCTGTGAGGAGAGCTCCCAGCAGAAGGCACGGAGTCAGA GCCAAAGTGCAGCCCCAAGCCAGAAAGAAAGAAATGGAGATGAGGCAGCTGCAGAGAGGC CCTGCCTGAAACCCACAGTGCCAGGCAACCCTTCAGAAAGGCTTTGCTCCTGCTCTCAGA TCAGATCAAGCATTCTTTCTATTTTATTATCTGGGACATATTTAAATACAAACATATTCA GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC027897 unedited GATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCANATCTGGTACCGGTCCGGA ATTCCCGGGATTGCAAAAAACCTTTCAAAAGCTTTCCTGGATTAGAGAAAGAAAGGGAGT GAGGGAGGAGAGATGAGTGGCTATTCCAGAACGACATAAAGAATTTCCAGCCTTGGACGG ACAGCTGGGAACGTCTTCCAATTTGGACTGGTGTTTACAAGCGGGAAGCTAGGTGGACCT TGGATTTTGGCGGGTGAAGAGGCTAGGTTGTTTAAGGAGGTGGGGCGCGTTTCAGTGGCT CTCTTTGAAAAAGCCCAGCAAGATGTCAGACCTGCTCTCAGTCTTCCTCCACCTCCTCCT TCTCTTCAAGTTGGTTGCCCCGGTGACCTTTCGCCACCACCGCTATGATGATCTTGTGCG GACGCTGTACAAGGTGCAAAACGAATGCCCCGGCATCACGCGGGTCTACAGCATTGGGCG CAGCGTGGAGGGGAGACACCTCTACGTGCTGGAGTTCAGCGACCACCCTGGAATCCACGA GCCCTTGGAACCAGAGGTCAAGTATGTGGNGAACATGCACGGCAACGAAGCGTTGGGCCG CGAGCTGATGCTGCAGCTGTCGGAGTTTCTGTGCGAGGAGTTCCGGAACANGAACCAGCG CATCGTCCAGCTCATCCAGGACACGCGCATTCACATCCTGCCATCCATGAACCCCCGACG CTACAAGGTGGCTGCTGCCCAGGGCCAAAACAGCCTGGGTATCTAGTTGGCAGGGAACAT GCAAATGGGAGTGGACCTGAACCCGCACTTCCTGGATCTCATACCTATTTCTACTTTACG AGGAGTACGGGAGGCCCCAACAACACCTGGCCCCTTCCGAAACTGGAAAAATCAGGTGGG AACCCGAACCCGGGCCGGGGATCCGGGGGAGCCCTCCTTCCACTTTGGTCTTTAACCCAA TTCCCCGGGGGG
Restriction Sites	NotI-NotI
ACCN	BC027897
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027897.1, AAH27897.1
RefSeq Size	1784 bp
Locus ID	1369
Protein Families	Druggable Genome, Protease, Secreted Protein
Gene Summary	Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]

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