HSPC210 (GSKIP) (BC004818) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	chromosome 14 open reading frame 129; GSK3beta interaction protein; GSKIP; HSPC210; MGC4945
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC004818 edited CAGCTCGGTGCTGATTATCACAACTGTTTGGTGACCTACTTCACTGACCTGTGAGGATTC CTTCCCTTCAAGTACTGGATTCTTGATCTTTCTGCATCATCAAGAATGGAAACAGACTGT AATCCCATGGAGCTAAGCAGTATGTCAGGATTTGAAGAAGGTTCAGAGCTGAACGGTTTT GAAGGAACTGACATGAAAGACATGAGGCTCGAAGCTGAAGCAGTTGTAAATGATGTTCTC TTTGCTGTTAACAACATGTTTGTCTCGAAAAGCCTGCGGTGTGCGGATGATGTGGCCTAT ATCAATGTGGAAACAAAGGAAAGAAACAGATATTGCCTAGAACTCACTGAAGCAGGGCTC AAGGTGGTAGGCTATGCTTTTGACCAGGTAGATGATCATTTACAGACTCCCTACCATGAA ACAGTCTACTCCTTGTTGGATACACTCAGCCCCGCCTACCGAGAAGCATTTGGAAACGCA CTGCTTCAAAGACTGGAAGCTTTGAAAAGAGATGGACAGTCATGACTACACTTTTTCCTT TCAGAGGGGCTGGTGCTCGTACAGAATGTTGATATAAAGCTTAAAATTCTTGCATATGGT CATAGAAAATGCATCTTTGGTTTTGTGTTTTTATCACTTGCTTCCAACTTAGGCTTTTGG CTCAGAAGATTATTGAATAATGATTTGTCTTAGTTTCTGTTTCAGTAAGGGAATTCTGAG GCCGTTGCTATGATACCATCATTAAGACATTCACATGTCTTCATATAATATCTCTTCATT TCAAATCCTAATCACTATTTCATACTATTACAGGGCTTTGATGCTGCCAGCACTGTCTTT TACATAGGAAATTCTAGATTTGCACAGTAATAGAGGAATTAGAAGTACCTAACTATACAC TTTGATTCAGCCTGCTAAATCAGGGGTTCAATACTAGCTTGGACAAACTTTGTAGTAATT AATTGCTACCAGCCTTATTGGAAACAAATTATCAACTAGTTTCCCCTGCACAAATTTTGA AATTCACTGCTTCACTTAATCTATTTATATTACTAATAATGGATTAATAAAGATGAATTA ATTATATATTACTTAACTAGTATTAAATGAAAAACAGGGACTGAAATAGTTCTGTATTCC GTGTTTGCAACAGCCAGCCAACTAAGCAGAGGATAAACCGTTAGCAAATGAATGTAATAA TTACTCATTTCCAAGATATCTAAGCACATAAGCAAATACAGGAACAGACTTCATTCTTTT TCTTAACAAAAAAGCATCTTCAGTGTGTGATTTAAAAGAAAGAAGATTCTGGTTTCCTAG AAAACAATATTTTGGCCTGTGTTGATTCTTATTCTGAATGTGTGTTTACATAATGTACAG TATATATTCAGAAAGTATTTTTGCTTCAACGTTTACTTTCTATGATGTAGTGCTTTGGTA TTCCTACAGCACCCCACCTTCCCCAACAGATGTACAGTGTTCTGTCTCCATTCGAAATCT ACAATGTAATATGAGTGCATTGTATGGGTTTGAAACCAAAGGATGAATGAAGCATTCAGA GACTTAATATTTGAAAAAGGAATAGTCAGTATTTTATATTTTATTACAGGTACTGATATT TATAAATTTAATAAACTGTACCATGCTGCTGCATGTTTTCAAGTACATGTTGAACAGTAA GGATTGGGGAGTTGTTTTTTAATGGTCACCTAAAGCAGCTGCTATAGAAATGTTGAACTA AAATTTTGCATCTGGTCATACCTTCATGCATTTATCATTTGCAGATATTTTTCCATCATT ATTAAAAAACAGGAACTTTTAGGCTCTGAAGATCATGTGGACCAGAGCAAATTAAAGTTC AGTTTGTGTCACAATTCATTGCCAGACTTCATTGGAATGCTTTGTTTGATGATGTATGTT CATTCTCAGCTTTATTTTCAGATGCTTAACTGGGCAATGAAGTCTAACTTCAGGTTGAAC TTTCTCATGTTTAATCTCAGGCTAAATGTAAATGATATTTGTAAAGTTTGAATAAAATTC TGTTTACTCATTTTGAGTTAGTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC004818 unedited GGGTACCTTGATTTGTAAACCACTTACTATAGGCGGCCGCGNAATTCGATCTGGTACCGG TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGAAGAAGAGGACGCCGGGCGCG CAGCTCGGTGCTGATTATCACAACTGTTTGGTGACCTACTTCACTGACCTGTGAGGATTC CTTCCCTTCAAGTACTGGATTCTTGATCTTTCTGCATCATCAAGAATGGAAACAGACTGT AATCCCATGGAGCTAAGCAGTATGTCAGGATTTGAAGAAGGTTCAGAGCTGAACGGTTTT GAAGGAACTGACATGAAAGACATGAGGCTCGAAGCTGAAGCAGTTGTAAATGATGTTCTC TTTGCTGTTAACAACATGTTTGTCTCGAAAAGCCTGCGGTGTGCGGATGATGTGGCCTAT ATCAATGTGGAAACAAAGGAAAGAAACAGATATTGCCTAGAACTCACTGAAGCAGGGCTC AAGGTGGTAGGCTATGCTTTTGACCAGGTAGATGATCATTTACAGACTCCCTACCATGAA ACAGTCTACTCCTTGTTGGATACACTCAGCCCCGCCTACCGAGAAGCATTTGGAAACGCA CTGCTTCAAAGACTGGAAGCTTTGAAAAGAGATGGACAGTCATGACTACACTTTTTCCTT TCAGAGGGGCTGGTGCTCGTACAGAATGTTGATATAAAGCTTAAAATTCTTGCATATGGT CATAGAAAATGCCATCTTGGGTTTGTGTTTTTATCACTTGCTTCCAACTTAGGCTTTTGG CTCAGAAGATATTGAATAAATGATTGTCCTAATTTCTGTTTCAATAAGGGAAATCTGAAG CCCTTGCTATGATACCCTCTTTAAAACATCCCATGTCTTCATATATATCTCTCC
Restriction Sites	NotI-NotI
ACCN	BC004818
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004818.2, AAH04818.1
RefSeq Size	2110 bp
Locus ID	51527
Gene Summary	This gene encodes a protein that is involved as a negative regulator of GSK3-beta in the Wnt signaling pathway. The encoded protein may play a role in the retinoic acid signaling pathway by regulating the functional interactions between GSK3-beta, beta-catenin and cyclin D1, and it regulates the beta-catenin/N-cadherin pool. The encoded protein contains a GSK3-beta interacting domain (GID) in its C-terminus, which is similar to the GID of Axin. The protein also contains an evolutionarily conserved RII-binding domain, which facilitates binding with protein kinase-A and GSK3-beta, enabling its role as an A-kinase anchoring protein. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]

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