SH2D1B (BC066595) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EAT2; OTTHUMP00000029531; SH2 domain-containing molecule EAT2; SH2 domain containing 1B
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC066595 edited GGTACAGCTGCTTACACATCTCTCCTCAGAGCTGTCCCTTGACTTGGGGGTGAATTTCAG GCCAACAGGGCTTCCTGGGATACAAGAGCGTTCTCCATGGATCTGCCTTACTACCATGGA CGTCTGACCAAGCAAGACTGTGAGACCTTGCTGCTCAAGGAAGGGGTGGATGGCAACTTT CTTTTAAGAGACAGCGAGTCGATACCAGGAGTCCTGTGCCTCTGTGTCTCGTTTAAAAAT ATTGTCTACACATACCGAATCTTCAGAGAGAAACACGGGTATTACAGGATACAGAACAGT AACAGCGATTATGTGGATGTCTTGCCTTGAAGATAAGGCTGCCGGACAAAGCAAGTTGAA GAGATGAGTAACAGTTCTCACTGATGACCCACTTCTGCAGGCATAGGTCCAGAGCACCAA ACTCTAGTGGACAATTCAGACTCTCCTGGTTGTGTAACTGAAGATGTTCTGCCCACCAGC ACCAGAGGTCACTCTCCACATCCCCGCCTCCCAGACATATACCAGGAGCAATTTCAAAAC CCTCTCCAGTTTCACTCTTCTTTCTTGGAATGGGACAGCCTGAACATTTTCCCTTTGACT TGTTAAAGGTACCACCCTACATCATGGTTGACACCCTCCTTTGGACCATGCAGTCAGAGG GGCAGCTTTATACAGAGGAGGGGCACACTTGTCTGTGAGTTTGAAGCCCTGAGTTCCAGT CCTGTGGCTGTGTGACTTTGAACACGTTTCTGCCCATCTCCAGGTCTTAGTTTCTTTGTC TCTGTGGTTGGGTGGGATGATAAACATTGCCCCAGTCTCTCTGTTGAGCCTGCTTGTGTC AGGTGAAGGTGAGGGATCGGGAGTGATGGAGATGCATACAGATCAGCACCTTCTCTGTAC CTGCTGGACCCCCATGTGCACCCTTCTCCCTCCAGCCTGGGCTACTCTCCTCCTGGTTCT TTCTAATCTTAAACTTCCTGGTGAGCATTGCCCCAGCCACACTTTCTCCCTGGTTTTGCT TAATTCCACTTATGCATCAGTCATTTCAACAGCATGAATGTGGTTCTGTACAGTGATGTA TGTGGAGATTTCTAAAATATTCTATGTGTGATTTCTGTTCCCAACTAGGTTCTAAGTGCC ATCAAAGCACGGTTGGGTCTCCCTTGTATCTTCCACAGTGTGTAGAAATTTGTGCTGCCC AGAGCTGTGCCTAGTTCATAGAAACTGACTGGAAGCACATTGCTGAGGGAGGTTTGTTGA ATTGGTTTTTAAGGTTTATTGAAATTGATTTGCTGAATTTTTCTGCTAGTTCAAAATGTG AATTAGGACCTGGTCAGTTTGAAATATACCAAATTCTATGCCCTTTCTCTTACCTATCAT CAAATTGTAGTAATGTATTTCACCCCACTGGACTTATCTTCAGAGTTTTAAAAGAGAGGA GCTCTCGACTTAGAGGTAATATGAACAGATGAACAGACACTGTGGCTGGAGCCCCAAAGT GTGGAGCATTGTGAGATTTGGGGTCACAGCAATTTATGGCTACTATTCCCTGGGTCTGGT AGGTAGGACAAATGTCCTCTTTTACTTTTCAAAACTGGCTTAGGTATTCATGGACCTTGA TTCTTCTATATACATTTTATAATAAATACGTGAAATTGCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	Please inquire
ACCN	BC066595
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066595.1, AAH66595.1
RefSeq Size	1712 bp
Locus ID	117157
Protein Pathways	Natural killer cell mediated cytotoxicity
Gene Summary	By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.