PEPD (BC028295) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	imidodipeptidase; MGC10905; peptidase D; PROLIDASE; proline dipeptidase
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC028295 edited CGAACATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGC CGCTGGCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTG CTGTGCAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCA CCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTG AGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCA GGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGG AGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCAC AGAAGCCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCA GGGAGGCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAG AGATCGTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCA ATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAG AATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACA GCTCCTACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACG CCGGAGCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCG GTGAGTATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCA CTGCAGACCAGAAGGCCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTG CCATGAAGCCAGGTGTCTGGTGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGG AGGAGCTGGCCCACATGGGCATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACC TGGGGGCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACG TGGGAGGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCA CTGCACGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCG ACCACCTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCTTTAACCGCGAGG TCCTGCAGCGCTTTCGCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTG ACAGCGGCATAGAGCTGCTGACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCA TGGCAGGCTGTGACAAGGCCTTTACCCCCTTCTCTGGCCCCAAGTAGAGCCAGCCAGAAA TCCCAGCGCACCTGGGGGCCTGGCCTTGCAACCTCTTTCCGTGATGGGCAGCCTGCTGGT CAGCACTCCAGTAGCGAGAGACGGCACCCAGAATCAGATCCCAGCTTCGGCATTTGATCA GACCAAACAGTGCTGTTTCCCGGGGAGGAAACACTTTTTTAATTACCCTTTTGCAGGCTC CCACCTTTAATCTGTTTCATACCTTGCTTATTAAATGAGCGACTTAAAATGATTGAAAAT AATGCTGTTCTTTAGTAGCAACTAAAATGTGTCTTGCTGTCATTTATATTCCTTTTCCCA GGAAAGAAGCATTTCTGATACTTTACTTTCTGTCAAAAATCAATATGCAGAATGGCATTT GCAATAAAAGGTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC028295 unedited GAGTTTGAATTTGTATACGACTTATATAGGCGGCCGCGNAATTCNATCTGGTACCGGTCC GGAATTCCCGGGATATCGTCGACCCACGCGTCCGCGAACATGGCGGCGGCCACCGGACCC TCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTGAACCGG CAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGTC CTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAG GAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGAT GTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGG ATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAGTATGCCGTGGACGACGTCCAG TACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAGCCCTCTGTCCTCCTCACTTTG CGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTGACGGCATCAGC AAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAAG ACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGT GAGGTAATGAAGGCTGTANAAGTGGGAATGAAAGATATGAGTTGGNAAGCCTCTTCGAGC ACTACTGCTACTCCCGGGCGGCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGG TGAGGACTCAGCCGTGCTACACTACCGACACGCCGGGACTNCCNACGACCA
Restriction Sites	NotI-NotI
ACCN	BC028295
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028295.1, AAH28295.1
RefSeq Size	1908 bp
Locus ID	5184
Protein Families	Druggable Genome, Protease
Gene Summary	This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

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