Neurofilament (NEFL) (BC066952) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; neurofilam; neurofilament, light polypeptide 68kDa; neurofilament-light; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; NF-L; NF68; NFL
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC066952 edited GCACACAGCCATCCATCCTCCCCCTTCCCTCTCTCCCCTGTCCTCTCTCTCAGGGCTCCC ACCGCCGCCGCGGGCCGGGGAGCCACCGGCCGCCACCATGAGTTCCTTCAGCTACGAGCC GTACTACTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGGTGCACATCTCCAG CGTGCGCAGCGGCTACAGCACCGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGTGTC TTCCTCGCTGTCCGTGCGCCGCAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCT GGAGAACCTCGACCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCAC GCAGGAGAAGGCGCAGCTCCAGGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGT GCACGAGCTGGAGCAGCAGAACAAGGTCCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAA GCACTCCGAGCCATCCCGCTTCCGGGCGCTGTACGAGCAGGAGATCCGCGACCTGCGCCT GGCGGCGGAAGATGCCACCAACGAGAAGCAGGCGCTCCAGGGCGAGCGCGAAGGGCTGGA GGAGACCCTGCGCAACCTGCAGGCGCGCTATGAAGAGGAGGTGCTGAGCCGCGAGGACGC CGAGGGCCGGCTGATGGAAGCGCGCAAAGGCGCCAAGAACACCGACGCCGTGCGCGCCGC CAAGGACGAGGTGTCCGAGAGCCGTCGTCTGCTCAAGGCCAAGACCCTGGAAATCGAAGC ATGCCGGGGCATGAATGAAGCGCTGGAGAAGCAGCTGCAGGAGCTGGAGGACAAGCAGAA CGCCGACATCAGCGTTATGCAGGACACGATCAACAAATTAGAAAATGAATTGAGGACCAC AAAGAGTGAAATGGCACGATACCTAAAAGAATACCAAGACCTCCTAACGTGAAGATGGCT TTGGATATTGAGATTGCAGCTTACAGGAAACTCTTGGAAGGCGAGGAGACCCGACTCAGT TTCACCAGCGTGGGAAGCATAACCAGTGGCTACTCCCAGAGCTCCCAGGTCTTTGGCCGA TCTGCCTACGGCGGTTTACAGACCAGCTCCTATCTGATGTCCACCCGCTCCTTCCCGTCC TACTACACCAGCCATGTCCAAGAGGAGCAGATCGAAGTGGAGGAAACCATTGAGGCTGCC AAGGCTGAGGAAGCCAAGGATGAGCCCCCCTCTGAAGGAGAAGCCGAGGAGGAGGAGAAG GACAAGGAAGAGGCCGAGGAAGAGGAGGCAGCTGAAGAGGAAGAAGCTGCCAAGGAAGAG TCTGAAGAAGCAAAAGAAGAAGAAGAAGGAGGTGAAGGTGAAGAAGGAGAGGAAACCAAA GAAGCTGAAGAGGAGGAGAAGAAAGTTGAAGGTGCTGGGGAGGAACAAGCAGCTAAGAAG AAAGATTGAACCCCCATTTCCTTAATTATTTCAGGAATAATTCTCCCGAAATCAGGTCAA CCCCATCACCAACCAACCAACCAGTTGAGTTCCAGATTCTATGTGAATTAAAAAGTCAAT ATATGTATAATTCTGAGATGACTTAGGTTGGACATTCAATGTTGTGCTATGAATTTCCTC TTTATGCAGAGTATCTGTTTGCTTGCAGAGTGGCTTTCTGGCTTGCTGCCAGCCTGTGCA TGGTCCACGCTTATGAGTTCAGGATCTACGGCAATGTGAATCATTCAGATGTTTACAATA AAAAACACCACATGAGTAAATGAATTCACTAATGTTAATGTTAAACTTCATGGAAAAATA GTCCTTTGAACCTTCGGTGGTTAGCAATTAAAGACCCTGAGTTATGTGCAATAAATAGTA AATAAAGTTATACCGAACGAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC066952 unedited GAATTGGGGGTCGAATTTGTAAACGACTCATATAGGCGGCCGCATAACTTCGTATAGCAT ACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGG CACACAGCCATCCATCCTCCCCCTTCCCTCTCTCCCCTGTCCTCTCTCTCAGGGCTCCCA CCGCCGCCGCGGGCCGGGGAGCCACCGGCCGCCACCATGAGTTCCTTCAGCTACGAGCCG TACTACTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGGTGCACATCTCCAGC GTGCGCAGCGGCTACAGCACCGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGTGTCT TCCTCGCTGTCCGTGCGCCGCAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCTG GAGAACCTCGACCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCACG CAGGAGAAGGCGCAGCTCCAGGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGTG CACGAGCTGGAGCAGCAGAACAAGGTCCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAAG CACTCCGAGCCATCCCGCTTCCGGGCGCTGTACGAGCAGGAGATCCGCGACCTGCGCCTG GCGGCGGAAGATGCCACCAACGAGAAGCANGCGCTCCAGGGCGAGCGCGAAGGGCTGGAG GAGACCCTGCGCAACCTGCNAGCGCGCTATGAAGAGGAGGTGCTGAGCCGCGAGGACGCC CGAGGCCGGCTGATGGAAGCGCGCAAGGCGCCAAAACACCGACGCCGTGCGCGCCGCCNA GGACGAGGTGTCCC
Restriction Sites	NotI-NotI
ACCN	BC066952
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066952.1, AAH66952.1
RefSeq Size	1895 bp
Locus ID	4747
Protein Families	Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways	Amyotrophic lateral sclerosis (ALS)
Gene Summary	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

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