WDR77 (BC006477) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	androgen receptor cofactor p44; HKMT1069; MEP50; methylosome protein 50; MGC2722; Nbla10071; OTTHUMP00000013472; p44; RP11-552M11.3; WD repeat domain 77
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC006477 edited CGGAGCGGAGTCTCCGGCCGGCGTCCAGTTTGAGTCTAGGTTGGAGTTGGAACCGTGGAG ATGCGGAAGGAAACCCCACCCCCCCTAGTGCCCCCGGCGGCCCGGGAGTGGAATCTTCCC CCAAATGCGCCCGCCTGCATGGAACGGCAGTTGGAGGCTGCGCGGTACCGGTCCGATGGG GCGCTTCTCCTCGGGGCCTCCAGCCTGAGTGGGCGCTGCTGGGCCGGCTCCCTCTGGCTT TTTAAGGACCCCTGTGCCGCCCCCAACGAAGGCTTCTGCTCCGCCGGAGTCCAAACGGAG GCTGGAGTGGCTGACCTCACTTGGGTTGGGGAGAGAGGTATTCTAGTGGCCTCCGATTCA GGTGCTGTTGAATTGTGGGAACTAGATGAGAATGAGACACTTATTGTCAGCAAGTTCTGC AAGTATGAGCATGATGACATTGTGTCTACAGTCAGTGTCTTGAGCTCTGGCACACAAGCT GTCAGTGGTAGCAAAGACATCTGCATCAAGGTTTGGGACCTTGCTCAGCAGGTGGTACTG AGTTCATACCGAGCTCATGCTGCTCAGGTCACTTGTGTTGCTGCCTCTCCTCACAAGGAC TCTGTGTTTCTTTCATGCAGCGAGGACAATAGAATTTTACTCTGGGATACCCGCTGTCCC AAGCCAGCATCACAGATTGGCTGCAGTGCGCCTGGCTACCTTCCTACCTCGCTGGCTTGG CATCCTCAGCAAAGTGAAGTCTTTGTCTTTGGTGATGAGAATGGGACAGTCTCCCTTGTG GACACCAAGAGTACAAGCTGTGTCCTGAGCTCAGCTGTACACTCCCAGTGTGTCACTGGG CTGGTGTTCTCCCCACACAGTGTTCCCTTCCTGGCCTCTCTCAGTGAAGACTGCTCACTT GCTGTGCTGGACTCAAGCCTTTCTGAGTTGTTTAGAAGCCAAGCCCACAGAGACTTTGTG AGAGATGCGACTTGGTCCCCGCTCAATCACTCCCTGCTTACCACAGTGGGCTGGGACCAT CAGGTCGTCCACCACGTTGTGCCCACAGAACCTCTCCCAGCCCCTGGACCTGCAAGTGTT ACTGAGTAGATTGGATTTAAGACAAAAAGCAAGTCCCCCATGAGTGTCCACTTCTTTGCC CTGCCCTCTCAGCTTGTGAGACAACACAGGAGCCTTCTATAGTATGTTGATATGCTAGAT CTGTGCCGTTAATAGGCATCGTCTCTCAGCCTGAGGGAGGCTGGATTCTGGGTTCCTGTA GTCACAGGGAGGAAAAGCTTTCTTAAAAATGGACATGTATGTGCGTGTGAGTGTGTGTGT AGATTTATAGTTTTTGGTAGTGGCAGGAATAAAAAAAATCCATCCTACATCTTCCCTAAG CACTGCCTCTCTCTCACCCCCCAAAACAAGTTGACGAAAGGGTTTTATGTAGCTGTCTAT GAGGAATTGGCCGTGTCTGGGTGGGTTATGGGATGTGGGCATCCCTGGGTTCTTGGAAGC AGCTCTTATGCTACTCATAGAGATGGGATTGACTTTATTTTTTTATAGTGCTTAATTCAC CATTATGAGAAATGCTTCCAGTCACAAAAATGCAGCCCAGCTCACTCTGAGGAAGAAGCA GGACTTGGTACGGTTTTACACAACTCCTTACCATTAAACTGAATCAGAAATCCATTTTCT GGCTGAATAAAAAGTTTGGCTTGCCTGTGTAATGCCCACTCCCTTCCCCCTGGCTCCCTA GTGATGGGACATATATGAGAGAGAAGTGTTTTTCTATCATAGACACCATAGGGGAAAGTT TGGGGATGAAGGAGAGCTTAAAGGTGTTTCAATTAAGTTAGAAAACTGACACAGGCTGTT GAGAATTCTTTGCCACTTTTCCCACCCCAAAACAGCATGGGGCCTGACATCTTCTGCCCT GGTCCCCTTTCTCTTGATGTGGAAAGTCTGAATGCAGTATTTATAGACTTCTAAGGTTTT AAAATCCAGTATCAAGAAGAAAATCAGAAATACTGGTTGGTGAAATAAAGAGTTTAGGCA TTGTTAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC006477 unedited NAAGGTCAGACTTTGTAAACGACTCACTATAGGCGGCCGCGACATCGGCACGAGGGGGTT TGCTGCCGGAGCGGAGTCTCCGGCCGGCGTCCAGTTTGAGTCTAGGTTGGAGTTGGAACC GTGGAGATGCGGAAGGAAACCCCACCCCCCCTAGTGCCCCCGGCGGCCCGGGAGTGGAAT CTTCCCCCAAATGCGCCCGCCTGCATGGAACGGCAGTTGGAGGCTGCGCGGTACCGGTCC GATGGGGCGCTTCTCCTCGGGGCCTCCAGCCTGAGTGGGCGCTGCTGGGCCGGCTCCCTC TGGCTTTTTAAGGACCCCTGTGCCGCCCCCAACGAAGGCTTCTGCTCCGCCGGAGTCCAA ACGGAGGCTGGAGTGGCTGACCTCACTTGGGTTGGGGAGAGAGGTATTCTAGTGGCCTCC GATTCAGGTGCTGTTGAATTGTGGGAACTAGATGAGAATGAGACACTTATTGTCAGCAAG TTCTGCAAGTATGAGCATGATGACATTGTGTCTACAGTCAGTGTCTTGAGCTCTGGCACA CAAGCTGTCAGTGGTAGCAAAGACATCTGCATCAAGGTTTGGGACCTTGCTCAGCAGGTG GTACTGAGTTCATACCGAGCTCATGCTGCTCAGGTCACTTGTGTTGCTGCCTCTCCTCAC AAGACTCTGTGTTTCTTTCATGCAGCGAGGACAATAGAATTTTACTCTGGGATACCCGCT GTCCCAAGCCAGCATCACAGATTGGCTGCAGTGCGCCTGGCTACCTTCCTACCTCGCTGG CTTGGCATCCTCAGCAAAGTGAAGTCTTTGTCTTTGGTGATGAGAATGGGACAGTCTCCC TTGTTGACACCAAGAGTACAGCTGTGTCCTGAGCTCAGCTGTACACTCCCAGTGTGTCAC TGGGCTGGTGTTCTCCCCCACAGTGTTCCT
Restriction Sites	Please inquire
ACCN	BC006477
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC006477.1, AAH06477.1
RefSeq Size	2079 bp
Locus ID	79084
Protein Families	Stem cell - Pluripotency
Gene Summary	The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

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