MT1H (BC008408) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	metallothionein 1H; MGC70702; MT1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC008408 edited ACCACGCCCTCCACGTGTTCCACTGCCTCTTCTCTTCTCGCTTGGGAACTCCAGTCTCAC CTCGGCTTGCAATGGACCCCAACTGCTCCTGCGAGGCTGGTGGCTCCTGCGCCTGCGCCG GCTCCTGCAAGTGCAAGAAGTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGTT GCCCCCTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCAGAGAAGT GCAGCTGCTGTGCCTGATGTCGGGACAGCCCTGCTGTCAGATGAAAACAGAATGACACGT AAAATCCAGGATTTTTTTTTTTCTACAACTCCGACTCATTTGCTACATTCCTTTTTTTCT GTGAAATATGTGAATAATAATTAAACACTTAGACCAAAAAAAAAAAAAAAAAAAAAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008408 unedited TCATATTTGTAAACACTCATATAGGCGGCCGCGTAATTCGCCATTACGGCCGGGACCACG CCCTCCACGTGTTCCACTGCCTCTTCTCTTCTCGCTTGGGAACTCCAGTCTCACCTCGGC TTGCAATGGACCCCAACTGCTCCTGCGAGGCTGGTGGCTCCTGCGCCTGCGCCGGCTCCT GCAAGTGCAAGAAGTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGTTGCCCCC TGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCAGAGAAGTGCAGCT GCTGTGCCTGATGTCGGGACAGCCCTGCTGTCAGATGAAAACAGAATGACACGTAAAATC CAGGATTTTTTTTTTTCTACAACTCCGACTCATTTGCTACATTCCTTTTTTTCTGTGAAA TATGTGAATAATAATTAAACACTTAGACCAAAAAAAAAAAAAAAAAAAAAAAAAAACATG TCGGCCGCCTCGGCCCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAG ATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCA CTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGG TGTCCTTCTATAATATTATGGGGTGAGGGGGGTGGGTAATGGAACAAAGGGCAAANTTTT AAAAAAAAACTGGTAGGGCCTGCCGGTTCTATTGGGAAACCAACCTGGGATGCAGTGGCA CAAATCTTGGGCTCATGGATTCTCCCCCTCCCGGGGTTAAACGATTCTCCTGGCTAAACC CCCCGAATTGGTGGGATTCCCCAGCTGCGTGAACCAGCCTAAAATAATTTTTTTTTTTTT TGGAAAAAACCGGGGTTCACCCATTTGGGCCAGGCGGGGTTCCCACCTCCTAATCCGGGG GGACTCCCCCCCTTGC
Restriction Sites	NotI-NotI
ACCN	BC008408
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008408.1, AAH08408.1
RefSeq Size	422 bp
Locus ID	4496
Gene Summary	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.