Apc10 (ANAPC10) (NM_014885) Human Untagged Clone
CAT#: SC126079
ANAPC10 (untagged)-Human anaphase promoting complex subunit 10 (ANAPC10)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | APC10; DOC1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_014885 edited
GTTCAACGCCGTTGGCGAAGCCAGCTGCTGGAGGTGCCGAGAATCTGAGTTTCGGCAAGC AGCCAGAATATTTTAAAAATGACTACACCAAACAAGACACCTCCTGGTGCTGACCCCAAG CAGTTGGAAAGGACTGGAACAGTACGGGAAATTGGGTCACAAGCTGTTTGGTCACTCTCA TCTTGCAAACCAGGATTTGGAGTGGATCAGTTACGAGATGACAATCTAGAAACTTATTGG CAATCAGATGGTTCCCAGCCTCATTTAGTGAACATCCAATTCAGAAGAAAAACAACAGTG AAGACATTATGTATTTATGCAGACTACAAATCTGATGAAAGCTATACTCCAAGCAAGATC TCAGTCAGAGTAGGAAATAATTTTCACAACCTTCAAGAAATTCGGCAACTTGAGTTGGTG GAACCAAGTGGCTGGATTCATGTTCCCTTAACTGACAATCATAAGAAGCCAACTCGTACA TTCATGATACAGATTGCTGTTCTAGCCAATCACCAGAATGGAAGAGACACCCATATGAGA CAAATTAAAATATACACACCAGTAGAAGAGAGCTCCATTGGTAAATTTCCTAGATGTACA ACTATAGATTTCATGATGTATCGTTCAATAAGGTGACTTTAAAATGAGACGAAAATCATT AAACGTATCTTTGTTTTATCCTGTATTTAAATAATATATCATGTACCTTTATTGAACAAG GCATCCGTTATATCTAATTTTGTATATGTTTAAAAATATTTTATTGTAACTTTGACAAAT AAATTTGGGGTCATATTATCTTTATTTTCTTTAACATGTAATAAAGCTCACATATTTTAC ATTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_014885 unedited
GGGGCAGGATTTGTATACGACTCCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGG GGGTTCAACGCCGTTGGCGAAGCCAGCTGCTGGAGGTGCCGAGAATCTGAGTTTCGGCAA GCAGCCAGAATATTTTAAAAATGACTACACCAAACAAGACACCTCCTGGTGCTGACCCCA AGCAGTTGGAAAGGACTGGAACAGTACGGGAAATTGGGTCACAAGCTGTTTGGTCACTCT CATCTTGCAAACCAGGATTTGGAGTGGATCAGTTACGAGATGACAATCTAGAAACTTATT GGCAATCAGATGGTTCCCAGCCTCATTTAGTGAACATCCAATTCAGAAGAAAAACAACAG TGAAGACATTATGTATTTATGCAGACTACAAATCTGATGAAAGCTATACTCCAAGCAAGA TCTCAGTCAGAGTAGGAAATAATTTTCACAACCTTCAAGAAATTCGGCAACTTGAGTTGG TGGAACCAAGTGGCTGGATTCATGTTCCCTTAACTGACAATCATAAGAAGCCAACTCGTA CATTCATGATACAGATTGCTGGTCTAGCCAATCACCAGAATGGAAGAGACACCCATATGA GACAAATTAAAATATACACACCAGTAGAAGAGAGCTCCATTGGGAAATTTCCTAGATGTA CAACTATAGATTTCATGATGTATCGTTCAATAAGGTGACTTTAAAATGAGACGAANATCA TTAAACGTATCTTTGGTTTATCCTGTGTTTAAATAATATATCATGTGACTTTATTGAACA GGGCGTCCGTTATATCTAATTTTGTATATGTGTAAAAATATTTTATTNGTGACTTTGAGC AATAAATTTGGGGTCATATTATCTTTTATTTTCTTTAACTGGAGATAAGCTCACATATTT TACATTACCCCNNNAAAAAAAAAAANAAAAAAAAAAACTGTGGGCCGCCTCGGG |
Restriction Sites | Please inquire |
ACCN | NM_014885 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014885.1, NP_055700.1 |
RefSeq Size | 941 bp |
RefSeq ORF | 558 bp |
Locus ID | 10393 |
UniProt ID | Q9UM13 |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
Gene Summary | ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011] Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202679 | ANAPC10 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 10 (ANAPC10) |
CNY 2,400.00 |
|
RC202679L1 | Lenti ORF clone of Human anaphase promoting complex subunit 10 (ANAPC10), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC202679L2 | Lenti ORF clone of Human anaphase promoting complex subunit 10 (ANAPC10), mGFP tagged |
CNY 5,890.00 |
|
RC202679L3 | Lenti ORF clone of Human anaphase promoting complex subunit 10 (ANAPC10), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202679L4 | Lenti ORF clone of Human anaphase promoting complex subunit 10 (ANAPC10), mGFP tagged |
CNY 5,890.00 |
|
RG202679 | ANAPC10 (tGFP-tagged) - Human anaphase promoting complex subunit 10 (ANAPC10) |
CNY 4,000.00 |