NKIAMRE (CDKL3) (BC041799) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cyclin-dependent kinase-like 3; NKIAMRE; serine-threonine protein kinase NKIAMRE
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC041799, the custom clone sequence may differ by one or more nucleotides ATGGAGATGTATGAAACCCTTGGAAAAGTGGGAGAGGGAAGTTACGGAACAGTCATGAAATGTAAACATA AGAATACTGGGCAGATAGTGGCCATTAAGATATTTTATGAGAGACCAGAACAATCTGTCAACAAAATTGC GATGAGAGAAATAAAGTTTCTAAAGCAATTTCATCACGAAAACCTGGTCAATCTGATTGAAGTTTTTAGA CAGAAAAAGAAAATTCATTTGGTATTTGAATTTATTGACCACACAGTATTAGATGAGTTACAACATTATT GTCATGGACTAGAGAGTAAGCGACTTAGAAAATACCTCTTCCAGATCCTTCGAGCAATTGACTATCTTCA CAGTAATAATATCATTCATCGAGATATAAAACCTGAGAATATTTTAGTATCCCAGTCAGGAATTACTAAG CTCTGTGATTTTGGTTTTGCACGAACACTAGCAGCTCCTGGGGACATTTATACGGACTATGTGGCCACAC GCTGGTATAGAGCTCCCGAATTAGTATTAAAAGATACTTCTTATGGAAAACCTGTGGATATCTGGGCTTT GGGCTGTATGATCATTGAGATGGCCACTGGAAATCCCTATCTTCCTAGTAGTTCTGATTTGGATTTACTC CATAAAATTGTTTTGAAAGTGGGCAATTTGTCACCTCACTTGCAGAATATCTTTTCCAAGAGCCCCATTT TTGCTGGGGTAGTTCTTCCTCAAGTTCAACACCCCAAAAATGCAAGAAAAAAATATCCAAAGCTTAATGG ATTGTTGGCAGATATAGTTCATGCTTGTTTACAAATTGATCCTGCTGACAGGATATCATCTAGTGATCTT TTGCATCATGAGTATTTTACTAGAGATGGATTTATTGAAAAATTCATGCCAGAACTGAAAGCTAAATTAC TGCAGGAAGCAAAAGTCAATTCATTAATAAAGCCAAAAGAGAGTTCTAAAGAAAATGAACTCAGGAAAGA TGAAAGAAAAACAGTTTATACCAATACACTGCTAAGTAGTTCAGTTTTGGGAAAGGAAATAGAAAAAGAG AAAAAGCCCAAGGAGATCAAAGTCAGAGTTATTAAAGTCAAAGGAGGAAGAGGAGATATCTCAGAACCAA AAAAGAAAGAGTATGAAGGTGGACTTGGTCAACAGGATGCAAATGAAAATGTTCATCCTATGTCTCCAGA TACAAAACTTGTAACCATTGAACCACCAAACCCTATCAATCCCAGCACTAACTGTAATGGCTTGAAAGAA AATCCACATTGCGGAGGTTCTGTGACAATGCCACCCATCAATCTAACTAACAGTAATTTGATGGCTGCAA ATCTCAGTTCAAATCTCTTTCACCCCAGTGTGAGGTTAACTGAAAGAGCAAAAAAGAGACGCACTTCTTC ACAATCTATTGGACAAGTTATGCCTAATAGCAGGCAAGAGGATCCAGGTCCTATTCAAAGCCAAATGGAG AAGGGTATATTTAATGAGCGAACAGGTCACAGTGACCAAATGGCAAATGAGAACAAAAGGAAGCTGAATT TTTCCAGATCTGACAGGAAAGAATTCCATTTTCCAGAATTGCCTGTCACAATACAGTCAAAAGATACAAA AGGAATGGAAGTTAAACAGATAAAAATGCTGAAGAGGGAGTCAAAGAAAACAGAGTCATCTAAGATACCA ACTTTACTTAACGTGGATCAAAATCAAGAAAAACAAGAGGGTGGAGATGGCCATTGCGAGGGGAAGAATT TGAAGAGAAACAGGTTTTTTTTCTGGTAG
Restriction Sites	Please inquire
ACCN	BC041799
Insert Size	2200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC041799.1, AAH41799.1
RefSeq Size	2146 bp
RefSeq ORF	1779 bp
Locus ID	51265
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	The protein encoded by this gene is a member of cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This gene was identified as a gene absent in leukemic patients with chromosome 5q deletion. This loss may be an important determinant of dysmyelopoiesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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