Phospholipase A2 (PLB1) (BC042674) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hPLB; PLB; PLB/LIP
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC042674 edited CTTTGTTGACCATCTCCGCAATGCCTTGGACGTCCTGCATAGAGAGGTGCCCAGAGTCCT GGTCAACCTCGTGGACTTCCTGAACCCCACTATCATGCGGCAGGTGTTCCTGGGAAACCC AGACAAGTGCCCAGTGCAGCAGGCCAGCTTGAACCACTTGGAAGCAAAACAGAGACCCTG GACCTGAGAGCAGAGATGCCCATCACCTGTCCCACTCAGAATGAGCCCTTCCTGAGAACC CCTCGGAATAGTAACTACACGTACCCCATCAAGCCAGCCATTGAGAACTGGGGCAGTGAC TTCCTGTGTACAGAGTGGAAGGCTTCCAATAGTGTTCCAACCTCTGTCCACCAGCTCCGA CCAGCAGACATCAAAGTGGTGGCCGCCCTGGGTGACTCTCTGACTACAGCAGTGGGAGCT CGACCAAACAACTCCAGTGACCTACCCACATCTTGGAGGGGACTCTCTTGGAGCATTGGA GGGGATGGGAACTTGGAGACTCACACCACACTGCCCAACATTCTGAAGAAGTTCAACCCT TACCTCCTTGGCTTCTCTACCAGCACCTGGGAGGGGACAGCAGGACTAAATGTGGCAGCG GAAGGGGCCAGAGCTAGGGACATGCCAGCCCAGGCCTGGGACCTGTTAGAGCGAATGAAA AACAGCCCCGACATCAACCTGGAGAAAGACTGGAAGCTGGTCACACTCTTCATTGGGGTC AACGACTTGTGTCATTACTGTGAGAATCCGGAGGCCCACTTGGCCACGGAATATGTTCAG CACATCCAACAGGCCCTGGACATCCTCTCTGAGGAGCTCCCAAGGGCTTTCGTCAACGTG GTGGAGGTCATGGAGCTGGCTAGCCTGTACCAGGGCCAAGGCGGGAAATGTGCCATGCTG GCAGCTCAGAACAACTGCACTTGCCTCAGACACTCGCAAAGCTCCCTGGAGAAGCAAGAA CTGAAGAAAGTGAACTGGAACCTCCAGCATGGCATCTCCAGTTTCTCCTACTGGCACCAA TACACACAGCGTGAGGACTTTGCGGTTGTGGTGCAGCCTTTCTTCCAAAACACACTCACC CCACTGAACGAGAGAGGGGACACTGACCTCACCTTCTTCTCCGAGGACTGTTTTCACTTC TCAGACCGCGGGCATGCCGAGATGGCCATCGCACTCTGGAACAACATGCTGGAACCAGTG GGCCGCAAGACTACCTCCAACAACTTCACCCACAGCCGAGCCAAACTCAAGTGCCCCTCT CCTGAGAGCCCTTACCTCTACACCCTGCGGAACAGCCGATTGCTCCCAGACCAGGCTGAA GAAGCCCCCGAGGTGCTCTACTGGGCTGTCCCAGTGGCAGCGGGAGTCGGCCTTGTGGTG GGCATCATCGGGACAGTGGTCTGGAGGTGCAGGAGAGGTGGCCGGAGGGAAGATCCTCCA ATGAGCCTGCGCACTGTGGCCCTCTAGGCCCGGGGGTGGGTCCTCACCCTAAACTCCCTA TAGCCACTCTCTTCACCGCCCTCTGCCCCAGCCACTCCCGGCCACCAGGACATGCTTCAA TGCCTGGTGCCATAGGAAGCCCAGGGGACAGTCACAACTTCTTGGGGCCTGGGCTTCTTC CAGGCCTATGCTCCTGGAATGGATACATTTAAATAAAGTCCAAAGCTATTTTAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC042674 unedited GAGGTTCACTACGCCTGGNANCGCCATCCACGCTGTTTTGACCTCCTAGAAGACACCGGG ACCGATCCAGCCTCCGGACTCTAGCCTAGGCCGCGGGACGGATAACAATTTCACACAGGA AACAGCTATGACCATTAGGCCTATTTAGGTGACACTATAGAACAAGTTTGTACAAAAAAG CAGGCTGGTACCGGTCCGGAATTCCCGGGATCTTTGTTGACCATCTCCGCAATGCCTTGG ACGTCCTGCATAGAGAGGTGCCCAGAGTCCTGGTCAACCTCGTGGACTTCCTGAACCCCA CTATCATGCGGCAGGTGTTCCTGGGAAACCCAGACAAGTGCCCAGTGCAGCAGGCCAGCT TGAACCACTTGGAAGCAAAACAGAGACCCTGGACCTGAGAGCAGAGATGCCCATCACCTG TCCCACTCAGAATGAGCCCTTCCTGAGAACCCCTCGGAATAGTAACTACACGTACCCCAT CAAGCCAGCCATTGAGAACTGGGGCAGTGACTTCCTGTGTACAGAGTGGAAGGCTTCCAA TAGTGTTCCAACCTCTGTCCACCAGCTCCGACCAGCAGACATCAAAGTGGTGGCCGCCCT GGGTGACTCTCTGACTACAGCAGTGGGAGCTCGACCAAAAACTCCAGTGACCTACCCAAT CTTGGAGGGGACTCTTTTGAACCATGGAGGGGATGGGAACTTGAAACTCACACCACACTG CCCAACATTTTGAAAAAGTTCAACCCTTACCTCTTTGGCTTCTCTACCACACCTGGGAAG GGACAGCAAGACTAAATGTGGCAGCGGAGGGGGCCCGAGCTAGGGACTGCCCCCCAAGCC TGGGACCTGTTAAAGCAATGAAAACAGCCCCGACATTAACCTGGAGAAAACTGG
Restriction Sites	NotI-NotI
ACCN	BC042674
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC042674.1, AAH42674.1
RefSeq Size	1790 bp
Locus ID	151056
Protein Families	Transmembrane
Gene Summary	This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene. [provided by RefSeq, Jul 2016]

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