HSPC111 (NOP16) (BC032424) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HBV pre-S2 trans-regulated protein 3; HSPC111; HSPC185; NOP16 nucleolar protein homolog; NOP16 nucleolar protein homolog (yeast); nucleolar protein 16 homolog
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC032424 edited GGACGCGTGGGCTGGTGCGGTGGTCAGCGCGATGCCCAAGGCCAAGGGCAAAACCCGGAG GCAGAAGTTTGGTTACAGTGTCAACCGAAAGCGTCTGAACCGGAATGCTCGACGGAAGGC AGCGCCGCGGATCGAATGCTCCCACATCCGACATGCCTGGGACCACGCTAAATCGGTACG GCAGAACCTGGCCGAGATGGGGTTGGCTGTGGACCCCAACAGGGCGGTGCCCCTCCGTAA GAGAAAGGTGAAGGCCATGGAGGTGGACATAGAGGAGAGGCCTAAAGAGCTTGTACGGAA GCCCTATGTGCTGAATGACCTGGAGGCAGAAGCCAGCCTTCCAGAAAAGAAAGGAAATAC TCTGTCTCGGGACCTCATTGACTATGTACGCTACATGGTAGAGAACCACGGGGAGGACTA TAAGCAGAGTGGCAAGACTTCCTCGATTCTTTGCAGAAGAGGAAGATGGAGGTGGAGTGA CTGGTTTACATCACAGCTGCCCCAGGCTGAGGCGTCCCCCGGACCAGTGAAGCTGGAGCC AGGGTGTAAGGCAAGGAGGTGCTGTGTGGCTCCAGAGGAGCTGGCCAGGTCCCATGGAAT CAGAAGGTTACACACACACGTGCACACTCCCCGCTCTGGGGAAGGAACTGTTCTCAGAGG CTCCAATTTATATTCATCTGGGGGTTCACGGAAAAGCCAGAACCTGCTGTTTTCAGGGTG GGTGATGTAAATATAGTGTGTACATAATAAAGCAAATATATTTTAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032424 unedited TTCATATTTTGTAATACGACTTCACTATAGGGCGGACCGCGTAATTCAGCATCTGGCTAC CGGCTCCGGTAATTCCCGGGATATCGTCGACCCACGCGTCCGCGGACGCGTGGGCTGGTG CGGTGGTCAGCGCGATGCCCAAGGCCAAGGGCAAAACCCGGAGGCAGAAGTTTGGTTACA GTGTCAACCGAAAGCGTCTGAACCGGAATGCTCGACGGAAGGCAGCGCCGCGGATCGAAT GCTCCCACATCCGACATGCCTGGGACCACGCTAAATCGGTACGGCAGAACCTGGCCGAGA TGGGGTTGGCTGTGGACCCCAACAGGGCGGTGCCCCTCCGTAAGAGAAAGGTGAAGGCCA TGGAGGTGGACATAGAGGAGAGGCCTAAAGAGCTTGTACGGAAGCCCTATGTGCTGAATG ACCTGGAGGCAGAAGCCAGCCTTCCAGAAAAGAAAGGAAATACTCTGTCTCGGGACCTCA TTGACTATGTACGCTACATGGTAGAGAACCACGGGGAGGACTATAAGCAGAGTGGCAAGA CTTCCTCGATTCTTTGCAGAAGAGGAAGATGGAGGTGGAGTGACTGGTTTACATCACAGC TGCCCCAGGCTGAGGCGTCCCCCGGACCAGTGAAGCTGGAGCCAGGGTGTAAGGCAAGGA GGTGCTGTGTGGCTCCAGAGGAGCTGGCCAGGTCCCATGGAATCAGAAGGTTACACACAC ACGTGCACACTCCCCGCTCTGGGGAAGGAACTGTTCTCAGAGGCTCCAATTTATATTCAT CTGGGGGTTCAACGGAAAGCCCAGAACCTGCTGTTATCAGGTGGGTGATGTAATATAGTG TGTACATAATAAAGCAATATATTTTAAAAAAAAAAAAAGGGCGGCCGCCGTCATAGCTGG TTTCTGAACATTCCCCGGTGGCATCCCTGTGACCCCTCCCAATGC
Restriction Sites	NotI-NotI
ACCN	BC032424
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032424.2, AAH32424.1
RefSeq Size	779 bp
Locus ID	51491
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein that is localized to the nucleolus. Expression of this gene is induced by estrogens and Myc protein and is a marker of poor patient survival in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

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