USP5 (BC005139) Human Untagged Clone

CAT#: SC125841

USP5 (untagged)-Human ubiquitin specific protease 5 (isopeptidase T), mRNA (cDNA clone MGC:1586 IMAGE:3506801), complete cds



CNY 3,816.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Anti-USP5 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ISOT; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ubiquitin isopeptidase T; ubiquitin specific peptidase 5; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5 (isopeptidase T)
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for BC005139 edited
GCCGTGTGTGGAGAAGCTGCTGCCGGTGTCATGGCGGAGCTGAGTGAGGAGGCGCTGCTG
TCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGACCGGGTCCACAAAGACGAGTGC
GCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGTATGAACACGTTT
CTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTAC
TTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGAC
CCACCCCGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGC
GAGGAGAAGTTTGAATTAGACGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAG
ATTGCCCGGGATGGACTGGGGGGACTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCA
GTGGAGGCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGCAGGAGGTGCAGGCATGGGAT
GGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGACAACCCTGCT
CGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTC
AACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAAC
AACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACC
ATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCC
AGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGACAGAC
AAGACGATGACTGAGTTGGAGATAGACATGAACCAGCGGATTGGTGAATGGGAGCTGATC
CAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGGCCTGGCTACACAGGCATCCGGAAC
CTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTC
CAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCCCCGACGGACCCTACC
CAGGATTTCAGCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTCTCCGGGGAGTATTCC
AAGCCAGTACCGGAGTCGGGCGATGGGGAGCGGGTGCCAGAACAGAAGGAAGTTCAAGAT
GGCATTGCCCCTCGGATGTTCAAGGCCCTCATCGGCAAGGGCCACCCTGAATTCTCCACC
AACCGGCAGCAGGATGCCCAGGAGTTCTTCCTTCACCTTATCAACATGGTGGAGAGGAAT
TGCCGGAGCTCTGAAAATCCTAATGAAGTGTTCCGCTTCTTGGTGGAGGAAAAGATCAAG
TGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTTGACTACATCATGCAGCTGCCT
GTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTACGAGGAGAAGAAGCGG
CAAGCCGAAGAGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGC
TCTTGCCTGGAGGCCTACGGGGCCCCTGAGCAGGTCGATGACTTCTGGAGCACGGCCCTG
CAGGCCAAGTCAGTAGCTGTCAAGACCACACGATTTGCCTCATTCCCTGACTACCTGGTC
ATCCAGATCAAGAAGTTCACCTTCGGCTTAGACTGGGTGCCCAAGAAACTGGATGTGTCC
ATCGAGATGCCAGAGGAGCTCGACATCTCCCAGTTGAGGGGCACAGGGCTGCAGCCCGGA
GAGGAGGAGCTGCCAGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGGTAGC
CTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCCCCTCACTTCTCCTCTCCG
ACATCGCCCATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGATTCCCTATG
GACGCCTGCCGCAAAGCTGTCTACTACACGGGCAACAGCGGGGCTGAGGCCGCCATGAAC
TGGGTCATGTCACACATGGATGATCCAGATTTTGCAAACCCCCTCATCCTGCCTGGCTCT
AGTGGGCCGGGCTCCACAAGCGCAGCAGCCGACCCCCCTCCTGAGGACTGTGTGACCACC
ATTGTCTCCATGGGCTTCTCCCGGGACCAGGCCTTGAAAGCGCTGCGGGCCACGAACAAT
AGTTTAGAACGGGCTGTGGACTGGATCTTCAGTCACATTGACGACCTGGATGCTGAAGCT
GCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGACTCCATCTCTGAGTCTGTGCCAGTG
GGACCTAAAGTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATG
GGCACCTCTACCATGTGTGGTCACTACGTCTGCCACATCAAGAAAGAAGGCAGATGGGTG
ATCTACAATGACCAGAAAGTGTGTGCCTCCGAGAAGCCGCCCAAGGACCTGGGCTACATC
TACTTCTACCAGAGAGTGGCCAGCTAAGAGCCTGCCTCACCCCTTACCAATGAGGGCAGG
GGAAGACCACCTGGCATGAGGGAGAGGGGCTGAGGGATGGACTTCAGCCCCTCTGCTCTG
TACCCTTTTTCCTTTTGTCCCCGGCAGCAGGGAAGAAGCTGGAGGCCGTGGGAGAATGGC
TGGGCAGAGCAGAGGGGCAGCGATAGACTCTGGGGATGGAGCAGGACGGGGACGGGAGGG
GCCGGCCACCTGTCTGTAAGGAGACTTTGTTGCTTCCCCTGCCCCCGGAATCCACAGTGC
TCTGCTTCTCTGTGTCGCCCCGCCCAGCCCCCTGGTGTGGAGGGAGGGGTCTCGTTTGTG
CGCGTGGGTGTAGCTTTGTGCATCCTCTCCCAGTGGAGCGATCACCTGTGCCTCCCCTCC
CCCTTTGTTTGCCCCTGTGTGGTTGGTCAAGGAGGGATGTGAGGGAAATAGGGACCCCCC
GACTTGCCCTCCTGCCTCAGTCTTTCCCCCACCCTGTCTCTTCCTTGTCCTTCTCTGGAA
AATGCCAAAATACACGATGTGAATAAAAGTACAACGGCTAAAAAAAAAAAAAAAAAAAAA
AAAAA
>OriGene 5' read for BC005139 unedited
TTCAGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCCGTG
TGTGGAGAAGCTGCTGCCGGTGTCATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTA
TTACCGACGATCCGGGTCCCTAAGGCTGGAGACCGGGTCCACAAAGACGAGTGCGCCTTC
TCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGTATGAACACGTTTCTGGGC
TTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTACTTGCAC
CTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCC
CGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGCGAGGAG
AAGTTTGAATTAGACGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCC
CGGGATGGACTGGGGGGACTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAG
GCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGCAGGAGGTGCAGGCATGGGATGGGGAA
GTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGGACACCCTGCTCGAATC
CCTCCTGTGGCTGGAAGTGCNTCAAGTGTGACATGAGAGAGGACCTGTGGCTCAACCTGA
CTGATGGGCTCATCCTCTGTGGGCGACGCTACTTCGATGGGCGTGGGGGCAACANCCACG
CTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCCACATCACCC
CTGATGGAGCTGACGTGTACTCATATGATGAAGATGACATGGTCCTGGACCCCAGCCTGG
CTGACCCCTGTCCCACTTCGCATCAACTGCTGAAATGCCAAAACAACAACAA
Restriction Sites Please inquire     
ACCN BC005139
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC005139.2, AAH05139.1
RefSeq Size 3185 bp
Locus ID 8078
Protein Families Druggable Genome, Protease
Gene Summary Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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