ZNF207 (BC002372) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp761N202; OTTHUMP00000163700; zinc finger protein 207
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC002372 edited CGGAGCGGGGAACGAGGCCGTCGGCCATTTTGTGTCTGCTTCCTGTGGGACGTGGTGGTA GCCGTTGGGTTGGGAAAGTGAGGGATTTTTGGCCTCGTTTCTCCTGCTTCTTTTCTCCTC CCTTTTACTTTGCCGGTAGAACACAGTTATGGGTCGCAAGAAGAAGAAGCAGCTGAAGCC GTGGTGCTGGTATTGTAATAGAGATTTTGATGATGAGAAGATCCTTATTCAGCACCAAAA AGCAAAGCATTTTAAATGCCATATATGTCACAAGAAATTGTATACAGGACCTGGCTTAGC TATTCATTGCATGCAGGTACATAAAGAAACAATAGATGCCGTACCAAATGCAATACCTGG AAGAACAGACATAGAGTTGGAAATATATGGTATGGAAGGTATTCCAGAAAAAGACATGGA TGAAAGACGACGACTTCTTGAACAGAAAACACAAGAAAGTCAAAAAAAGAAGCAACAAGA TGATTCTGATGAATATGATGATGACGACTCTGCAGCCTCAACTTCATTTCAGCCACAGCC TGTTCAACCTCAGCAAGGTTATATTCCTCCAATGGCACAGCCAGGACTGCCACCAGTACC AGGAGCACCAGGAATGCCTCCAGGCATACCTCCATTAATGCCAGGTGTTCCTCCTCTGAT GCCAGGAATGCCACCAGTTATGCCAGGCATGCCACCTGGATTGCATCATCAGAGAAAATA CACCCAGTCATTTTGCGGTGAAAACATAATGATGCCAATGGGTGGAATGATGCCACCTGG ACCAGGAATACCACCTCTGATGCCTGGAATGCCACCAGGTATGCCCCCACCTGTTCCACG TCCTGGAATTCCTCCAATGACTCAAGCACAGGCTGTTTCAGCGCCAGGTATTCTTAATAG ACCACCTGCACCAACAGCAACTGTACCTGCCCCACAGCCTCCAGTTACTAAGCCTCTTTT CCCCAGTGCTGGACAGGCTCAGGCAGCTGTCCAAGGACCTGTTGGTACAGATTTCAAACC CTTAAATAGTACCCCTGCAACAACTACAGAACCCCCAAAGCCTACATTCCCTGCTTATAC ACAGTCTACAGCTTCAACAACTAGTACAACAAATAGTACTGCAGCTAAACCAGCGGCTTC AATAACAAGTAAGCCTGCTACACTTACAACAACTAGTGCAACCAGTAAGTTGATCCATCC AGATGAGGATATATCCCTGGAAGAGAGAAGGGCACAGTTACCTAAGTATCAACGTAATCT TCCTCGGCCAGGACAGGCCCCCATCGGTAATCCACCAGTTGGACCAATTGGAGGTATGAT GCCACCACAGCCAGGCATCCCACAGCAACAAGGAATGAGACCCCCAATGCCACCTCATGG TCAGTATGGTGGTCATCATCAAGGCATGCCAGGATACCTTCCTGGTGCTATGCCCCCGTA TGGGCAGGGACCGCCAATGGTGCCCCCTTACCAGGGTGGGCCTCCTCGACCTCCGATGGG AATGAGACCTCCTGTAATGTCGCAAGGTGGCCGTTACTGATCTTACTTCATCCAGTCTAA TAGGTTTGGAGATTAAACCTTTTCTCAACTTGTGCTGTTTATATAGCCAAGCTTCCGTCA ATAAGGCTTCATTGTGACTTTAACAAACATTATCTTCCCACATACCAGGAACTATTGGAC ATTTATTTTACATGGGAAAAATTATTTGGAATAATAAAGCAGGAACTTTTCCTGAAGTTG CAATTTATACTGTATGGCTTCTTTTTCATGTTTCATCTAGGTTTTTAGAAGTGAAGTATA GTAAATTTGGTTCGTTAAATTGTGAAGGCGCTGGAATTACATGAACATACCACCCTAGTA AAGGCAAGTTCTGTAAGCTTACATTGCTATTTGTAAAGTTTGCCTTCACAGCATTTCAGA TGCTGTTGGACTTCATGTCCCCAACCTAGCTTGGTGAGGGCTGTAACTGTTTCCAAGTAC TTGTACATTGGAAGTCTGAATGTGTAACAATATTTAATGTATTTAGAGTTCCTCATGTTG CAGGGTTTAAGAAATCTGACCCACCAAGGTCATGTGACTTTTCTGTACTGTTAAACTTCA TTGTAATAAAATGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002372 unedited NNNCCCCGTTCAGCTTTTGTAATACGACTCACTATAGNNGGCGGCCGCGATTCGGCACGA GGCGGAGCGGGGAACGAGGCCGTCGGCCATTTTGTGTCTGCTTCCTGTGGGACGTGGTGG TAGCCGTTGGGTTGGGAAAGTGAGGGATTTTTGGCCTCGTTTCTCCTGCTTCTTTTCTCC TCCCTTTTACTTTGCCGGTAGAACACAGTTATGGGTCGCAAGAAGAAGAAGCAGCTGAAG CCGTGGTGCTGGTATTGTAATAGAGATTTTGATGATGAGAAGATCCTTATTCAGCACCAA AAAGCAAAGCATTTTAAATGCCATATATGTCACAAGAAATTGTATACAGGACCTGGCTTA GCTATTCATTGCATGCAGGTACATAAAGAAACAATAGATGCCGTACCAAATGCAATACCT GGAAGAACAGACATAGAGTTGGAAATATATGGTATGGAAGGTATTCCAGAAAAAGACATG GATGAAAGACGACGACTTCTTGAACAGAAAACACAAGAAAGTCAAAAAAAGAAGCAACAA GATGATTCTGATGAATATGATGATGACGACTCTGCAGCCTCAACTTCATTTCAGCCACAG CCTGTTCAACCTCAGCAAGGTTATATTCCTCCAATGGCACAGCCAGGACTGCCACCAGTA CCAGGAGCACCAGGAATGCCTCCAGGCATACCTCCATTAATGCCANGTGTTCCTCCTCTG ATGCCAGGAATGCCACCAGTTATGCCAGGCATGCCACCTGGATTGCATCATCAGAGAAAT ACACCCAGTCATTTTGCGGTGAAAACATAATGATGCCCATGGGTGGAATGATGCCCCCTG GACCAGGATACCACCTCTGATGCCTGGAATGCCACCAGTATGCCCCCACCTGTTCCACC
Restriction Sites	Please inquire
ACCN	BC002372
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC002372.2, AAH02372.1
RefSeq Size	2167 bp
Locus ID	7756
Protein Families	Transcription Factors
Gene Summary	Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).[UniProtKB/Swiss-Prot Function]

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