PUF60 (BC009734) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FBP interacting repressor; FIR; FLJ31379; fuse-binding protein-interacting repressor; poly-U binding splicing factor PUF60; pyrimidine tract binding splicing f; RoBPI; Ro ribonucleoprotein-binding protein 1; Ro ribonucleoprotein binding protein 1; SIAHBP1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009734 edited GCGCGGCGTGTGGCCCTTGCGGGCGAGCCTGGCGGGGTTGCGTTCCTGTCCGCTAGCAGG TCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGGCGGCAGTGGTGGCAGCGG GAGACAAATGGAAACCTCCACAGGGCACAGACTCCATCAAGATGGAGAACGGGCAGAGCA CAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCCTTCAGAAGG CCAAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAGCAGACCATCGCGC ACCAGCAGCAGCAGCTCACCAACCTGCAGATGGCGGCTCAGCGGCAGCGGGCGCTGGCCA TCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGGGGGAGGACACCATCCGCC AGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGACTCCGTCACCA TGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCACAGCTGGCCT TGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAAGGTGGGCAGACCCAGCA ACATAGGGCAGGCCCAGCCCATCATAGACCAGTTGGCTGAGGAGGCACGGGCCTTCAACC GCATCTACGTGGCCTCTGTGCACCAGGACCTCTCAGACGATGACATCAAGAGCGTGTTTG AGGCCTTTGGCAAGATCAAGTCCTGCACACTGGCCCGGGACCCCACAACTGGCAAGCACA AGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCCCAAGATGCTGTGTCTTCCA TGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGCAAGGCTGTCACACCGCCCA TGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCTGCCGCTGCTGTGGCAGCTG CTGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCCGGAGCAGCGGTGCTGGGTA CCCTGGGCACACCTGGACTGGTGTCCCCAGCACTGACCCTGGCCCAGCCCCTGGGCACTT TGCCCCAGGCTGTCATGGCTGCCCAGGCACCTGGAGTCATCACAGGTGTGACCCCAGCCC GTCCTCCTATCCCGGTCACCATCCCCTCGGTGGGAGTGGTGAACCCCATCCTGGCCAGCC CTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAAGAAGAGGAGCTGTTTCCCG AGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATGAGCATCTCGGGCAGTAGCG CCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAGTCTACAGTGATGGTTCTGC GCAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAAGGGGAGGTGACAGAGGAGT GTGGCAAGTTCGGGGCCGTGAACCGCGTCATCATCTACCAAGAGAAACAAGGCGAGGAGG AGGATGCAGAAATCATTGTCAAGATCTTTGTGGAGTTTTCCATAGCCTCTGAGACTCATA AGGCCATCCAGGCCCTCAATGGCCGCTGGTTTGCTGGCCGCAAGGTGGTGGCTGAAGTGT ACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCGTGACAGTGGTCCCTCTCCCCGG ACTTGCACTTGTTCCTTGTTTCCTCTGGGTTTTATAGTGATACAGTGGTGTCCCCGGGGC CAGGCGCGCTCTGCCCAGCCCAGCCTACAGTGCGGATAAAGGTGCGGATGCTGCTGGCCC TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009734 unedited ACTATGAGGGNCGCGCCGCGTAATTCCCGGGAATATCGTACGACCCACGCGTCCGGCGCG GCGTGTGGCCCTTGCGGGCGAGCCTGGCGGGGTTGCGTTCCTGTCCGCTAGCAGGTCAAT GGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGGCGGCAGTGGTGGCAGCGGGAGAC AAATGGAAACCTCCACAGGGCACAGACTCCATCAAGATGGAGAACGGGCAGAGCACAGCC GCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCCTTCAGAAGGCCAAG AAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAGCAGACCATCGCGCACCAG CAGCAGCAGCTCACCAACCTGCAGATGGCGGCTCAGCGGCAGCGGGCGCTGGCCATCATG TGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGGGGGAGGACACCATCCGCCAGGCC TTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGACTCCGTCACCATGAAG CACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCACAGCTGGCCTTGGAG CAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAAGGTGGGCAGACCCAGCAACATA GGGCAGGCCCAGCCCATCATAAACCANTTGGCTGANGAGGCACGGGCCTTCAACCGCATC TACGTGGCCTCTGTGCACCAGGACCTCTCAGACGATGACATCAAGAGCGTGTTTGAGGCC TTTGGGCAGATCAAGTCCTGCACACTGGCCCGGNACCCCNACACTGGCAAGCACAAGGGC TACNGGCTTCATTGAGTACGAGAAGGCCCCAGTCGTCCAAGATGCTGTGTCCTTCATGAA CCTCTTTGACCT
Restriction Sites	Please inquire
ACCN	BC009734
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009734.1, AAH09734.1
RefSeq Size	1837 bp
Locus ID	22827
Protein Pathways	Spliceosome
Gene Summary	This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

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