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SPO11 (NM_198265) Human Untagged Clone
CAT＃: SC125697
SPO11 (untagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT35; SPATA43; TOPOVIA; TOPVIA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_198265 edited
CAAGGGCGCAGCCTAGGACAGGGGCTTCTGGAGCTTCTGGCAGCCGTCTGCCCTCATGGC CTTTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACACAGGGAGTC CCTGCTGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAACTGGGGGAAGCCGCCTGGC CTCCAGGTTTGAAGATTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACCAGAAAGAT CAAAAGTGATTCACCAAAATCAGCTCAAAAATTTTCTCTAATCCTTAAAATATTGTCCAT GATTTATAAATTAGTACAGAGCAACACTTATGCAACCAAAAGGGACATATATTACACTGA CAGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAATGACATTTCTTGCAT GTTAAAAGTGTCAAGGAGGAGTCTACATATATTATCTACATCAAAAGGTTTAATTGCTGG CAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGTGCAACGGC TGTTGCTGTGCCATCGAATATTCAAGGAATTCGGAATTTAGTTACAGATGCAAAGTTTGT ATTAATTGTAGAAAAAGATGCAACATTTCAGCGGCTCCTAGATGACAACTTTTGCAACAA ATTGTCTCCTTGCATCATGATTACGGGAAAGGGAGTTCCTGATCTAAACACAAGACTTTT AGTCAAGAAACTGTGGGATACATTTCATGTTCCTGTTTTCACTCTTGTAGATGCTGATCC ACATGGCATAGAAATAATGTGCATCTATAAGTATGGATCTATGTCTATGTCTTTTGAAGC TCATCATCTCACAGTTCCAGCTATTAGATGGCTTGGTCTTCTCCCTTCTGATCTTAAAAG ATTAAATGTACCTAAAGATAGTTTGATTCCACTGACAAAAAGGGACCAAATGAAACTTGA CAGTATCCTGAGGAGACCTTATGTTACCTGCCAACCATTTTGGAGAAAAGAAATGGAAAT AATGGCAGACTCTAAAATGAAGGCAGAAATTCAAGCTTTGACTTTCCTATCATCAGATTA TCTTTCCAGAGTGTACTTACCTAACAAATTAAAATTTGGAGGATGGATATAAAAATAAAT CAGAAGAACTTCTGATTGCCAGAGGCTTTTCATTAGTTTTGTTTTGATTGGCAAATACTA TTGTGGAAAGAACATATATTATATTCTTAATTCTGTAAAAGTGAAATAAAATAACTTTCC GTTAATTATATATTTTTGTCAAAACAAATGCTGTACTCCAATTTTCTTTGCAAGGCCTTA TTCTTGCCTCTATAGAGACAGATTTCTGTCCTATCTTCTAAAGCAAATTATAAAAGAATA TGTTATTTTGACCTTTAAATTATTTTTGAAAAAATAATATTTTATACATGTCATCAAAGT CTACAAAATATTTACCTTCTACGATACAACTAATGTTAACGCATAAAGTATCTTACTGGT AACAAAAATCATAATGATCTGAATTTGAGATGTTGCAAATGAATTGTGGTGTCCGGTAGT TTCTTCTTACATTTTCCTTTGCCTTTATACTTTAGGGGTCTTACTCCATTAATTCATTTG TTACATTAGTAAAATTCAGTATGAATAAATATTTGGATTGATGTAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_198265 unedited
GTTTTATTTGTATACCATCATATAGGCGGACCGCGAATTCGATCTGGTACCGGTCCGGAT TCCCGGGATCAAGGCGCAGCCTAGGACAGGGGCTTCTGGAGCTTCTGGCAGCCGTCTGCC CTCATGGCCTTTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACAC AGGGAGTCCCTGCTGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAACTGGGGGAAGC CGCCTGGCCTCCAGGTTTGAAGATTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACC AGAAAGATCAAAAGTGATTCACCAAAATCAGCTCAAAAATTTTCTCTAATCCTTAAAATA TTGTCCATGATTTATAAATTAGTACAGAGCAACACTTATGCAACCAAAAGGGACATATAT TACACTGACAGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAATGACATT TCTTGCATGTTAAAAGTGTCAAGGAGGAGTCTACATATATTATCTACATCAAAAGGTTTA ATTGCTGGCAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGT GCAACGGCTGTTGCTGTGCCATCGAATATTCAAGGAATTCGGAATTTAGTTACAGATGCA AAGTTTGTATTAATTGTAGAAAAAGATGCCACATTTCAGCGGCTCCTAGATGACAACTTT TGCAACANATTGTCTCCTTGCATCATGATTACCGGAAAGGGAGTTCCTGATCTANACACA AGACTTTTAGTCAAGAAACTGTGGGATACATTTCATGTTCCTGTTTTCACTCTTGTAGAT GCTGATCCACATGGCATAGAAAATATGTGCATCTATAAGTATGGATCTATGTCTATGTCT TTTGAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_198265 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198265.1, NP_937998.1 |
| RefSeq Size | 1712 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 23626 |
| UniProt ID | Q9Y5K1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) lacks an internal segment compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206998 | SPO11 (Myc-DDK-tagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2 |
CNY 3,656.00 |
|
| RC206998L3 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206998L4 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG206998 | SPO11 (tGFP-tagged) - Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2 |
CNY 4,370.00 |
