Archaemetzincin 2 (AMZ2) (BC050709) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	archaelysin family metallopeptidase 2; archaemetzincin-2; archaemetzincins-2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC050709 edited GGACGCGGTGCGCATGCGCGCCCAGACATGTCCGTCCTTGTAAGTTAAAAGCTTCCATGG GAGCCTTCCTTCCTAATCAAGATGCAAATAATACGGCACTCCGAACAGACACTAAAAACA GCTCTCATCTCAAAGAACCCAGTGCTTGTATCACAGTATGAGAAATTAGATGCTGGGGAA CAACGTTTAATGAATGAAGCCTTCCAGCCAGCCAGTGATCTCTTTGGACCCATTACCTTG CATTCTCCATCAGATTGGATCACCTCCCACCCTGAGGCTCCCCAAGACTTTGAACAGTTC TTCAGTGATCCTTACAGAAAGACACCCTCTCCAAACAAACGCAGCATTTATATACAGTCC ATTGGCTCTCTAGGAAACACCAGAATTATCAGTGAAGAATATATTAAATGGCTCACGGGC TACTGTAAAGCATATTTCTATGGCTTGAGAGTAAAACTCCTAGAACCAGTTCCTGTTTCT GTAACAAGATGTTCCTTTAGAGTCAATGAGAACACACACAACCTACAAATTCATGCAGGG GACATCCTGAAGTTCTTGAAAAAGAAGAAACCTGAAGATGCCTTCTGTGTTGTGGGAATA ACAATGATTGATCTTTACCCAAGAGACTCGTGGAATTTTGTCTTTGGACAGGCCTCTTTG ACAGATGGTGTGGGGATATTCAGCTTTGCCAGGTATGGCAGTGATTTTTATAGCATGCAC TATAAAGGCAAAGTGAAGAAGCTCAAGAAAACATCTTCAAGTGACTATTCAATTTTCGAC AACTATTATATTCCAGAAATAACTAGTGTTTTACTACTTCGATCCTGTAAGACTTTAACC CATGAGATCGGACACATATTTGGACTGCGACACTGCCAGTGGCTTGCATGCCTCATGCAA GGCTCCAACCACTTGGAAGAAGCTGACCGGCGCCCTCTAAACCTTTGCCCTATCTGTTTG CACAAGTTGCAGTGTGCTGTTGGCTTCAGCATTGTAGAAAGATACAAAGCACTGGTGAGG TGGATTGATGATGAATCTTCTGACACACCTGGAGCAACTCCAGAACACAGTCACGAGGAT AATGGGAATTTACCGAAACCCGTGGAAGCCTTTAAGGAATGGAAAGAGTGGATAATAAAA TGCCTGGCTGTTCTCCAAAAATGAGGACCTTCAAATAGGAGTGATTGAAATAAATAACTA CTTGCATGTTATGCTTTCATTTGGGTGGAATACTTCATTGGAATAAACTACTGATCTTGT GCTGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC050709 unedited CCAGACTTTGTATACGACTCACTATAGGCGGCCGCGACATCANATCTGGTACCGGTCCGG AATTCCCGGGATATCGTCGACCCACGCGTCCGGGACGCGGTGCGCATGCGCGCCCAGACA TGTCCGTCCTTGTAAGTTAAAAGCTTCCATGGGAGCCTTCCTTCCTAATCAAGATGCAAA TAATACGGCACTCCGAACAGACACTAAAAACAGCTCTCATCTCAAAGAACCCAGTGCTTG TATCACAGTATGAGAAATTAGATGCTGGGGAACAACGTTTAATGAATGAAGCCTTCCAGC CAGCCAGTGATCTCTTTGGACCCATTACCTTGCATTCTCCATCAGATTGGATCACCTCCC ACCCTGAGGCTCCCCAAGACTTTGAACAGTTCTTCAGTGATCCTTACAGAAAGACACCCT CTCCAAACAAACGCAGCATTTATATACAGTCCATTGGCTCTCTAGGAAACACCAGAATTA TCAGTGAAGAATATATTAAATGGCTCACGGGCTACTGTAAAGCATATTTCTATGGCTTGA GAGTAAAACTCCTAGAACCAGTTCCTGTTTCTGTAACAAGATGTTCCTTTAGAGTCAATG AGAACACACACAACCTACAAATTCATGCANGGGACATCCTGAAGTTCTTGAAAAAGAAGA AACCTGAAGATGCCTTCTGTGTTGNTGGAATAAACATGATTGANTCTTACCCAAGAGACT CGTGGAATTTTGTCTTTGGACAGGCCTCTTTGACAGATGGTGTGGGGATATTCAGCTTTG CCAGTATGGCAGTGATTTTATAGCATGCACTATAAAGGCAAGTGAAGAAGCTCAAGAAAC ATCTTCAGTGACTATTCATTTTCGACACTATATATCCCAGAATACTAGTGTTTACTACTT CGATCTGTAGACTTACCCATGAGATCGGACACTATTTGACTGCT
Restriction Sites	NotI-NotI
ACCN	BC050709
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050709.1, AAH50709.1
RefSeq Size	1310 bp
Locus ID	51321
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is a zinc metalloprotease that displays some activity against angiotensin-3. The encoded protein is inhibited by the aminopeptidase inhibitor amastatin, as well as by the general inhibitors o-phenanthroline and batimastat. Defects in this gene may be associated with lung tumorigenesis. [provided by RefSeq, Oct 2016]

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