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CH25H (NM_003956) Human Untagged Clone
CAT＃: SC125616
CH25H (untagged)-Human cholesterol 25-hydroxylase (CH25H)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C25H |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_003956 edited
AGCTGCCTTGCACAGCCTCGCAATGAGCTGCCACAACTGCTCCGACCCCCAGGTCCTTTG CAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCTGGGACCACCTGAGGAGCTGGGAGGCCCT CCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCACATACGTGGGCTTTTGCCT GCCCTTCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGCGCTACAAGAT CCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGCAGACCCTCTA CCAGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCAGCCCGGCCCT CCTGCCCCACGAAGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGTTCTGCCTGCT ACTCTTCGACATGGAGTTCTTCGTGTGGCACCTGCTGCACCACAAGGTGCCCTGGCTGTA CCGCACCTTCCACAAGGTGCACCACCAGAACTCGTCCTCGTTCGCGCTGGCAACGCAGTA TATGAGCGTCTGGGAACTGTTTTCTTTGGGCTTCTTCGACATGATGAACGTCACACTGCT CGGGTGCCACCCGCTCACCACCCTGACCTTCCACGTGGTCAACATCTGGCTTTCCGTGGA GGACCACTCCGGCTACAACTTCCCTTGGTCCACTCACAGACTGGTGCCCTTCGGGTGGTA CGGGGGTGTGGTGCACCACGACCTGCATCACTCTCACTTTAACTGCAACTTCGCTCCGTA CTTTACACACTGGGACAAAATACTGGGAACGCTGCGGACTGCATCTGTCCCAGCGCGGTG ATGTGGCTGCGGTGGGTGCCCCTAAGACTCGGGACTGCTGTGCCTTTCACACTTGAATGA AGAGAAACACCTGAGCTATATATTTTTTTAAAGCAACTAACTTATTGCTTTATGTTTATC TATGAAAACCATAGATAAAATCTGATGCATTTTTGTAATCTGACAAAGTAATTTACATAC TGTTTGTGTATCAATACAATTTTGTGTTCTTGGTATTCTTAGTCTAGCTCACCTCAATAG CCTTGAATCCTGCATATGAATTAGACATTCATCACTGGCATATTTAGAATATCTCTAAAA GGACTTGTTTGTAGAATAAGGAATTTTCTATGTTTCAAAGTGTTCTAAAACCTGGCTAAA AGAATGTATTTTTGTGGATGGTGTTGACTTCTGACTCTAAAAGCAATCAAACATGTTTCT GCTGGACAGTGACCAAGAATTATAGTACCTTCTTATATTTTTTTATAGAACTGTATATTT ATTTTGAAAGAAATGTTATTCGTGCTTTAAAAAGGAAAAAAAACCATGAATCAAATAAAA GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_003956 unedited
GTTCAGCGATATTTGTNAATACGCACTGCACTATAGGGGCGGGCCGCGCAATTCCGCCAT TACGGCCGGGGAGCTGCCTTGCACAGCCTCGCAGGAGCTGCCACAACTGCTCCGACCCCC AGGTCCTTTGCAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCTGGGACCACCTGAGGAGCT GGGAGGCCCTCCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCACATACGTGG GCTTTTGCCTGCGCGGCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGC GCTACAAGATCCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGC AGACCCTCTACCAGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCA GCCCGGCCCTCCTGCCCCACGAAGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGT TCTGCCTGCTACTCTTCGACATGGAGTTCTTCGTGTGGCACCTGCTGCACCACAAGGTGC CCTGGCTGTACCGCACCTTCCACAAGGTGCACCACCAGAACTCGTCCTCGTTCGCGCTGG CAACGCAGTATATGAGCGTCTGGGGACTGTTTTCTTTGGGCTTCTTCGACATGATGAACG TCACACTGGGTCGGGTGCGACCCGCTCACCACCCTGAGCTTCCACGTGGTCAACATCTGG GCTTTCCGCTGGAGGACCACTTCCGGCTACCAACTTCCCTTTGGGCCACTCACAGGACTG GTGGCCCCTTCGGTGGGGGACGGGGGGTGGTGGTGGCACCCACAACCCTGGGTCAACTCT CACTTTTAACTGGCAACTTTCGTTCCCGTACTTTTAACCACCTGGGGACCAAATAACTGG GGAAACCGTTCGGGAACTGGAATTCTGTTCCCCACACCCCGGTGAATGTTGGCTCTCCCG GGGGGTGGCCCCTCTAAAACATCTCGGGACTGGCGTGGCGCGCTTCCACACTTGAG |
| Restriction Sites | Please inquire |
| ACCN | NM_003956 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003956.2, NP_003947.1 |
| RefSeq Size | 1411 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 9023 |
| UniProt ID | O95992 |
| Protein Families | Transmembrane |
| Protein Pathways | Primary bile acid biosynthesis |
| Gene Summary | This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204716 | CH25H (Myc-DDK-tagged)-Human cholesterol 25-hydroxylase (CH25H) |
CNY 2,400.00 |
|
| RC204716L1 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC204716L2 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), mGFP tagged |
CNY 5,890.00 |
|
| RC204716L3 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204716L4 | Lenti ORF clone of Human cholesterol 25-hydroxylase (CH25H), mGFP tagged |
CNY 4,800.00 |
|
| RG204716 | CH25H (tGFP-tagged) - Human cholesterol 25-hydroxylase (CH25H) |
CNY 4,000.00 |
