GNMT (NM_018960) Human Untagged Clone

CAT#: SC125540

GNMT (untagged)-Human glycine N-methyltransferase (GNMT)



  "NM_018960" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Anti-GNMT mouse monoclonal antibody, clone OTI8A3 (formerly 8A3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-182mP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC125540 sequence for NM_018960 edited (data generated by NextGen Sequencing)
ATGGTGGACAGCGTGTACCGGACCCGCTCCCTGGGGGTGGCGGCCGAAGGGCTCCCGGAC
CAGTACGCGGACGGGGAGGCGGCGCGCGTGTGGCAGCTGTATATCGGAGACACCCGCAGC
CGCACCGCCGAGTACAAGGCATGGCTGCTTGGGCTGCTGCGCCAGCACGGCTGCCAGCGG
GTGCTCGACGTAGCCTGTGGCACTGGGGTGGACTCCATTATGCTGGTGGAAGAGGGCTTC
AGTGTGACGAGTGTGGATGCCAGTGACAAGATGCTGAAGTATGCACTTAAGGAGCGCTGG
AACCGGCGGCACGAGCCCGCCTTCGACAAGTGGGTCATCGAAGAAGCCAACTGGATGACT
CTGGACAAAGATGTGCCCCAGTCAGCAGAGGGTGGCTTTGATGCTGTCATCTGCCTTGGA
AACAGTTTCGCTCACTTGCCAGACTGCAAAGGGGACCAGAGTGAGCACCGGCTGGCGCTG
AAAAACATTGCGAGCATGGTGCGGGCAGGGGGCCTACTGGTCATTGATCATCGCAACTAC
GACCACATCCTCAGTACAGGCTGTGCACCCCCAGGGAAGAACATCTACTATAAGAGTGAC
TTGACCAAGGACGTCACAACATCAGTGCTGATAGTGAACAACAAGGCCCACATGGTGACC
CTGGACTATACGGTGCAGGTGCCGGGGGCTGGCCAGGATGGCTCTCCTGGCTTGAGTAAG
TTCCGGCTCTCCTACTACCCACACTGTCTGGCATCCTTCACGGAGCTGCTCCAAGCAGCC
TTCGGAGGTAAGTGCCAGCACAGCGTCCTGGGCGACTTCAAGCCTTACAAGCCAGGCCAA
ACCTACATTCCCTGCTACTTCATCCACGTGCTCAAGAGGACAGACTGA

Clone variation with respect to NM_018960.4
>OriGene 5' read for NM_018960 unedited
GACTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCAGGATGGTGGACAGCGTG
TACCGGACCCGCTCCCTGGGGGTGGCGGCCGAAGGGCTCCCGGACCAGTACGCGGACGGG
GAGGCGGCGCGCGTGTGGCAGCTGTATATCGGAGACACCCGCAGCCGCACCGCCGAGTAC
AAGGCATGGCTGCTTGGGCTGCTGCGCCAGCACGGCTGCCAGCGGGTGCTCGACGTAGCC
TGTGGCACTGGGGTGGACTCCATTATGCTGGTGGAAGAGGGCTTCAGTGTGACGAGTGTG
GATGCCAGTGACAAGATGCTGAAGTATGCACTTAAGGAGCGCTGGAACCGGCGGCACGAG
CCCGCCTTCGACAAGTGGGTCATCGAAGAAGCCAACTGGATGACTCTGGACAAAGATGTG
CCCCAGTCAGCAGAGGGTGGCTTTGATGCTGTCATCTGCCTTGGAAACAGTTTCGCTCAC
TTGCCAGACTGCAAAGGGGACCAGAGTGAGCACCGGCTGGCGCTGAAAAACATTGCGAGC
ATGGTGCGGGCAGGGGGCCTACTGGTCATTGATCATCGCAACTACGACCACATCCTCAGT
ACAGGCTGTGCACCCNCAGGGAAGAACATCTACTATAAGAGTGACTTGACCAAGGACGTC
ACAACATCAGTGCTGATAGTGAACAACANGGGCCACATGGTGACCCTGGACTATACNGTG
CAAGTGCCGGNNGCTGGCCAANNNATGCTCTCTGGCTTGAGTAAGTTNCGGCTCTCTACT
ACCANACCTGGTNGGAATCTTCACGGGAGCTGCTCAGCAACCTTCGAAGGTAAGTGCACN
CAGCGTCTTGGCGATTNNAGCTTTAAAGCAAGGCAAAACTAATCCNTG
Restriction Sites Please inquire     
ACCN NM_018960
Insert Size 1200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018960.4, NP_061833.1
RefSeq Size 1091 bp
RefSeq ORF 888 bp
Locus ID 27232
UniProt ID Q14749
Protein Families Druggable Genome
Protein Pathways Glycine, serine and threonine metabolism
Gene Summary The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644. [provided by RefSeq, Jan 2016]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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