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USP8 (NM_005154) Human Untagged Clone
CAT＃: SC125502
USP8 (untagged)-Human ubiquitin specific peptidase 8 (USP8), transcript variant 1
CNY 7,792.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HumORF8; PITA4; SPG59; UBPY |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_005154 edited
GCGAATTCGCACGAGGGTCCTGGTAGCCAACGCTAATTCTCCCTCGAGTTCTTGGGAGAT GGGCATTTGGCGAGAAGGCTGGCGTTAGTGAAGCGCGCCCGGCGTCACGGAAAGAAGCAC TTGTAAGGAAATATAGCATCCATTGTGAAAGTGGAAAAGTAAAGATAATTCATCATGCCT GCTGTGGCTTCAGTTCCTAAAGAACTCTACCTCAGTTCTTCACTAAAAGACCTTAATAAG AAGACAGAAGTTAAACCAGAGAAAATAAGCACTAAGAGTTATGTGCACAGTGCCCTGAAG ATCTTTAAGACAGCAGAAGAATGCAGATTAGATCGTGATGAGGAAAGGGCCTATGTACTA TATATGAAATACGTGACTGTTTATAATCTTATCAAAAAAAGACCTGATTTCAAGCAACAG CAGGATTATTTCCATTCAATACTTGGACCTGGAAACATCAAAAAAGCTGTCGAAGAAGCT GAAAGACTCTCTGAAAGCCTTAAATTAAGATATGAAGAAGCTGAAGTCCGGAAAAAACTT GAGGAAAAAGACAGGCAGGAGGAAGCACAGCGGCTACAACAAAAAAGGCAGGAAACAGGA AGAGAGGATGGTGGCACATTGGCTAAAGGCTCTTTGGAGAATGTTTTGGATTCCAAAGAC AAAACCCAAAAGAGCAATGGTGAAAAGAATGAAAAATGTGAGACCAAAGAGAAAGGAGCA ATCACAGCAAAGGAACTATACACAATGATGACGGATAAAAACATCAGCTTGATTATAATG GATGCTCGAAGAATGCAGGATTATCAGGATTCCTGTATTTTACATTCTCTCAGTGTTCCT GAAGAAGCCATCAGTCCAGGAGTCACTGCTAGTTGGATTGAAGCACACCTGCCAGATGAT TCTAAAGACACATGGAAGAAGAGGGGGAATGTGGAGTATGTGGTACTTCTTGACTGGTTT AGTTCTGCCAAAGATTTACAGATTGGAACAACTCTCCGGAGTCTGAAAGATGCACTTTTC AAGTGGGAAAGTAAAACTGTCCTGCGCAATGAGCCTTTGGTTTTAGAGGGAGGCTATGAA AACTGGCTCCTTTGTTATCCCCAGTATACAACAAATGCTAAGGTCACTCCACCCCCACGA CGCCAGAATGAAGAGGTGTCTATCTCATTGGATTTTACTTATCCCTCATTGGAAGAATCA ATTCCTTCTAAACCTGCTGCCCAGACGCCACCTGCATCTATAGAAGTAGATGAAAATATA GAATTGATAAGTGGTCAAAATGAGAGAATGGGACCACTGAATATATCAACTCCAGTTGAA CCAGTTGCTGCTTCTAAATCTGATGTTTCACCCATAATTCAGCCAGTGCCTAGTATAAAG AATGTTCCACAGATTGATCGTACTAAAAAACCAGCAGTCAAATTGCCTGAAGAGCATAGA ATAAAATCTGAAAGTACAAACCATGAGCAACAATCTCCTCAGAGTGGAAAAGTTATTCCT GATCGTTCCACCAAGCCAGTAGTTTTTTCTCCAACTCTCATGTTAACAGATGAAGAAAAG GCTCGTATTCATGCAGAAACTGCTCTTCTAATGGAAAAAAACAAACAAGAAAAAGAACTT CGGGAAAGGCAGCAAGAGGAACAGAAAGAGAAACTGAGGAAGGAAGAACAAGAACAAAAA GCCAAAAAGAAACAAGAAGCTGAAGAAAATGAAATTACAGAGAAGCAACAAAAAGCAAAA GAAGAAATGGAGAAGAAAGAAAGTGAACAGGCCAAGAAAGAAGATAAAGAAACCTCAGCA AAGAGGGGCAAAGAAATAACAGGAGTAAAAAGACAAAGTAAAAGTGAACATGAAACTTCT GATGCCAAGAAATCTGTAGAAGATAGGGGGAAAAGGTGTCCAACCCCAGAAATACAGAAA AAGTCAACAGGAGATGTGCCCCATACATCTGTGACAGGGGATTCAGGTTCAGGCAAGCCA TTTAAGATTAAAGGACAACCAGAAAGTGGAATTCTAAGGACAGGAACTTTTAGAGAGGAT ACAGACGATACCGAAAGAAATAAAGCTCAACGAGAACCTTTGACAAGAGCACGAAGTGAA GAAATGGGGAGGATCGTACCAGGACTGCCTTCAGGCTGGGCCAAGTTTCTTGACCCAATC ACTGGAACCTTTCGTTATTATCATTCACCCACCAACACTGTTCATATGTACCCACCGGAA ATGGCTCCTTCATCTGCACCTCCTTCCACCCCTCCAACTCATAAAGCCAAGCCACAGATT CCTGCTGAGCGGGATAGGGAACCTTCCAAACTGAAGCGCTCCTACTCCTCCCCAGATATA ACCCAGGCTATTCAAGAGGAAGAGAAGAGGAAGCCAACAGTAACTCCAACAGTTAATCGG GAAAACAAGCCAACATGTTATCCTAAAGCTGAGATCTCAAGGCTTTCTGCTTCTCAGATT CGGAACCTCAATCCTGTTTTTGGAGGTTCTGGACCAGCTCTTACTGGACTTCGTAACTTA GGAAATACTTGTTATATGAACTCAATATTGCAGTGCCTATGTAACGCTCCACATTTGGCT GATTATTTCAACCGAAACTGTTATCAGGATGATATTAACAGGTCAAATTTGTTGGGGCAT AAAGGTGAAGTGGCAGAAGAATTTGGTATAATCATGAAAGCCCTGTGGACAGGACAGTAT AGATATATCAGTCCAAAGGACTTTAAAATCACCATTGGGAAGATCAATGACCAGTTTGCA GGATACAGTCAGCAAGATTCACAAGAATTGCTTCTGTTCCTAATGGATGGTCTCCATGAA GATCTAAATAAAGCTGATAATCGGAAGAGATATAAAGAAGAAAATAATGATCATCTCGAT GACTTTAAAGCTGCAGAACATGCCTGGCAGAAACACAAGCAGCTCAATGAGTCTATTATT GTTGCACTTTTTCAGGGTCAATTCAAATCTACAGTACAGTGCCTCACATGTCACAAAAAG TCTAGGACATTTGAGGCCTTCATGTATTTGTCTCTACCACTAGCATCCACAAGTAAATGT ACATTACAGGATTGCCTTAGATTATTTTCCAAAGAAGAAAAACTCACAGATAACAACAGA TTTTACTGCAGTCATTGCAGAGCTCGACGGGATTCTCTAAAAAAGATAGAAATCTGGAAG TTACCACCTGTGCTTTTAGTGCATCTGAAACGTTTTTCCTACGATGGCAGGTGGAAACAA AAATTACAGACATCTGTGGACTTCCCGTTAGAAAATCTTGACTTGTCACAGTATGTTATT GGTCCAAAGAACAATTTGAAGAAATATAATTTGTTTTCTGTTTCAAATCACTACGGTGGG CTGGATGGAGGCCACTACACAGCCTATTGTAAAAATGCAGCAAGACAACGGTGGTTTAAG TTTGATGATCATGAAGTTTCTGATATCTCCGTTTCTTCTGTGAAATCTTCAGCAGCTTAT ATCCTCTTTTATACTTCATTGGGACCACGAGTAACTGATGTAGCCACATAAGGAGACATA GGTTATAAACTAGTTATCTTTTAAAAGGCTCAGCAACACAACTCTTGAAATGCTTATCAG GATAATGGTAGCTATAGCTGGCCATTTAGAGGAATTCTAGGACAGTGGGAGCTGTGTTAC TAGCACTATATAATTCCGGTCAGTGCTGACAAATAACATTTAACAAGTATTGCAGTAATC ATCACTTACAGGTACCATTTATTTCAAAACAACTTTTTTAGTCTGCTCCAAAGTTAAAAT AATTAACTAGCTAAGCATTATTATTCGACTGGTCTAAAAACTATTGTTATCTTTTTTTTT CCTTTTCACTGTTATGGCCTTTTCACATTTCTAAATCCCATCTTGATATACTATGAATAC TCTAGAATGATGTAAAGC >OriGene 5' read for NM_005154 unedited
ATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTCCTGGTAGCC AACGCTAATTCTCCCTCGAGTTCTTGGGAGATGGGCATTTGGCGAGAAGGCTGGCGTTAG TGAAGCGCGCCCGGCGTCACGGAAAGAAGCACTTGTAAGGAAATATAGCATCCATTGTGA AAGTGGAAAAGTAAAGATAATTCATCATGCCTGCTGTGGCTTCAGTTCCTAAAGAACTCT ACCTCAGTTCTTCACTAAAAGACCTTAATAAGAAGACAGAAGTTAAACCAGAGAAAATAA GCACTAAGAGTTATGTGCACAGTGCCCTGAAGATCTTTAAGACAGCAGAAGAATGCAGAT TAGATCGTGATGAGGAAAGGGCCTATGTACTATATATGAAATACGTGACTGTTTATAATC TTATCAAAAAAAGACCTGATTTCAAGCAACAGCAGGATTATTTCCATTCAATACTTGGAC CTGGAAACATCAAAAAAGCTGTCGAAGAAGCTGAAAGACTCTCTGAAAGCCTTAAATTAA GATATGAAGAAAGCTGAAGTCCGGAAAAAACTTGAGGAAAAAGACAGGCAGGAGGAAGCA CAGCGGCTACAACAAAAAAGGCAGGAAACAGGAAGAGAGGGATGGTGGCACATTGGCTAA AGGCTCTTTGGAGAAATGTTTTGGATTCCAAAGACAAAACCCAAAAGAGCAATGGTGAAA AGAATGAAAAATGTGAGACCAAAGAGAAAGGGAGCATCACAGCANAGGAACCTATACACA TGATGACGGGATAANACATCAGCTTGATTATATGGATGCTCGAAGAATGCAGATATCANN GATNCTGTATTTACATCCNTTCANGGGTCTGAGAAACCATCATNCCAGATCCTGCTTANT GGATGC >OriGene 3' read for NM_005154 unedited
TTGTCCGCGGCCGCATCTAAGATCGGTTTTTTTTTTTTTTTTTTGGCTTTTTGTTCTTGT TCTTCCTTCCTCAGTTTCTCTTTCTGTTCCTCTTGCTGCCTTTCCCGAAGTTCTTTTTCT TGTTTGTTTTTTTCCATTAGAAGAGCAGTTTCTGCATGAATACGAGCCTTTTCTTCATCT GTTAACATGAGAGTTGGAGAAAAAACTACTGGCTTGGTGGAACGATCAGGAATAACTTTT CCACTCTGAGGAGATTGTTGCTCATGGGTTGTACTTTCAGATTTTATTCTATGCTCTTCA AGCAATTTGACTGCTGGTTTTTTAGTACGATCAATCTGTGGAACATTCTTTATACTAGGC ACTGGCTGAATTATGGGTGAAACATCAGATTTAGAAGCAGCAACTGGTTCAACTGGAGTT GATATATTCAGTGGTCCCATTCTCTCATTTTGACCACTTATCAATTCTATATTTTCATCT ACTTCTATAGATGCAGGTGGCGTCTGGGCAGCAGGTTTAGAAGGAATTGATTCCTTCCAA TGAGGGATAAGTAAAATCCAATGAGATAGACACCTCTTCATTCTGGCGTCGTGGGGGTGG AGTGACCTTATCATTTGTTGTATACTGCGGATAACAAAGGAGCCAGTTTTCATAGCCTCC CTCTAAAACCAAAGGCTCATTGCGCAAGACAGTTTTACCTTCCCACTTGAATAGTGCATC TTTTAAACTCCCGGAGAGTTGTTCCCAATCTGTAAATCTTTCGGCAGAACTAAACCAGTC CAGTAATTCCACATACTCCACATTCCCCCTCTTCTTCCATGAGGCTTTAGAATCATCTGG CAGGGGGGCTTCATTCCACTAGCAGTGACCCCGGGACTGAAGGCCTCCTCTAGACCCTGG AAGATGTAAATTACAGAACCCCGATATCCTGCCTTCTTCGACTCTCTTATATTAAGCGGA GTTTTATCCGCACATTGGGTAAATCCCTCTGCGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005154 |
| Insert Size | 1890 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005154.2, NP_005145.2 |
| RefSeq Size | 4250 bp |
| RefSeq ORF | 3357 bp |
| Locus ID | 9101 |
| UniProt ID | P40818 |
| Domains | UCH, RHOD |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Endocytosis |
| Gene Summary | This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216926 | USP8 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 8 (USP8), transcript variant 1 |
CNY 7,784.00 |
|
| RC216926L1 | Lenti ORF clone of Human ubiquitin specific peptidase 8 (USP8), transcript variant 1, Myc-DDK-tagged |
CNY 10,184.00 |
|
| RC216926L2 | Lenti ORF clone of Human ubiquitin specific peptidase 8 (USP8), transcript variant 1, mGFP tagged |
CNY 7,600.00 |
|
| RC216926L3 | Lenti ORF clone of Human ubiquitin specific peptidase 8 (USP8), transcript variant 1, Myc-DDK-tagged |
CNY 10,184.00 |
|
| RC216926L4 | Lenti ORF clone of Human ubiquitin specific peptidase 8 (USP8), transcript variant 1, mGFP tagged |
CNY 10,184.00 |
|
| RG216926 | USP8 (tGFP-tagged) - Human ubiquitin specific peptidase 8 (USP8), transcript variant 1 |
CNY 9,384.00 |
