PPP2R3A (NM_002718) Human Untagged Clone
CAT#: SC125495
PPP2R3A (untagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1
CNY 8,016.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | PPP2R3; PR72; PR130 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_002718 edited
CACGAGGGGGCCTGCGGGTGGCTGGGCGGGGCGCGCTCGGGCCTCCGCTCTTCACGCGCC GCATTCGTAGCCCGAGAGTCCGCGCCGCGGGGAGGCTTGGAGGCAAGCGCTGCCCGCGAG CTGAGCCGCCGGAGGAGGAGCCGCGGGCAACGAGGTTTCTCTGTCATTCACAGAAAAATG AATCATTTAAACCTTTGGAGGACTCAGTTATCACAATACTTCTCTACTACCAACTAAGAT TTATGATAGTAAATTTATGAGAGCAAATTTCCATGTTATAGAACTGTTGAAGAACTAAAA GAGGATATTCTTTCATCAAAATAATTCTGCAGTATCATAATATTACTAAATAAAATTAAA AAGCACAATTATTAAATTATTAAATTTGCCACACATGTGCAGCAGCTACTGTATCCTGAT AGTGACCAAACCTCAAATATAAATGGTTTCCCTTCATGGGAAAAGCCATTATATTTGGAA GAAACCACTGAACATTGTTATTAAATATATTTTCAGCTAACTGTCATTTAGGAGAATTTT CATGAAACAAGTTCTAGAAAGTTCCAAGTCCCACCAGTAAGTGGATTTGATATTATGGCA GCAACTTACAGACTTGTGGTTAGTACTGTGAACCACTACAGCAGCGTGGTGATAGACCGG CGTTTTGAACAAGCTATACATTATTGCACTGGAACCTGCCACACCTTCACACATGGAATT GACTGCATTGTGGTACACCATAGTGTTTGTGCAGACCTCTTGCACATCCCTGTGTCTCAG TTCAAAGATGCAGATCTGAACTCTATGTTTCTACCCCATGAAAATGGGCTTTCTTCGGCT GAAGGAGACTATCCCCAACAGGCCTTCACAGGCATACCCAGGGTCAAGAGAGGATCTACA TTTCAGAATACCTACAACTTAAAGGATATTGCAGGAGAAGCAATCAGTTTTGCCAGTGGG AAAATAAAAGAATTTTCCTTTGAAAAACTCAAAAACTCTAACCATGCAGCTTACAGAAAG GGAAGGAAAGTTAAGTCTGACTCATTTAATAGGAGGTCAGTTGATTTGGACTTGCTTTGT GGCCATTATAACAACGATGGGAACGCCCCATCCTTTGGTTTACTGCGGAGTTCCTCAGTT GAGGAAAAACCTTTGTCTCATAGAAACTCACTGGATACGAACCTGACTTCCATGTTTCTT CAAAACTTTTCTGAAGAAGACTTGGTTACTCAGATTTTGGAAAAACATAAAATAGATAAT TTTTCTTCTGGGACAGACATAAAGATGTGCTTGGACATCTTATTGAAATGCTCCGAGGAT TTAAAAAAATGCACAGACATCATAAAACAATGCATAAAGAAAAAATCAGGGAGTAGCATC AGTGAAGGAAGTGGTAATGATACAATTTCTAGCTCTGAAACTGTCTATATGAATGTAATG ACCAGGTTAGCATCCTATCTGAAAAAGTTACCATTTGAATTCATGCAGTCTGGGAATAAT GAGGCTCTAGATTTAACAGAACTGATCAGTAATATGCCTAGCTTACAACTGACTCCCTTC TCCCCAGTGTTTGGCACTGAACAACCCCCTAAATATGAAGATGTTGTCCAGCTCTCAGCT TCTGACTCTGGACGATTTCAAACTATTGAATTGCAAAATGACAAGCCTAATTCTAGGAAG ATGGACACTGTACAATCCATTCCAAACAACTCCACAAATTCCTTATATAACTTAGAGGTA AATGATCCTAGAACTCTAAAAGCTGTCCAGGTCCAATCACAGTCATTAACCATGAATCCT TTAGAAAATGTTTCTTCTGACGACTTAATGGAAACTCTTTATATTGAAGAAGAGTCAGAT GGAAAGAAAGCATTAGATAAAGGACAAAAGACAGAGAATGGACCTAGTCATGAGTTATTA AAGGTAAATGAACATAGAGCAGAATTTCCAGAACATGCTACTCATCTTAAAAAATGCCCC ACCCCAATGCAAAATGAAATTGGTAAGATATTTGAGAAATCATTTGTTAATCTACCTAAG GAAGACTGTAAATCAAAAGTTTCTAAATTTGAAGAGGGAGACCAGAGAGATTTTACAAAT TCCAGTAGCCAGGAAGAGATAGATAAATTGTTAATGGATTTGGAATCTTTTTCACAGAAG ATGGAGACCTCTCTAAGAGAGCCACTTGCGAAGGGTAAAAACTCTAATTTTTTAAATAGT CACAGTCAGTTGACCGGTCAGACCCTTGTAGATCTTGAGCCTAAATCTAAAGTCTCTTCA CCCATAGAAAAAGTCTCACCTTCCTGTCTAACAAGGATTATTGAAACCAATGGACACAAA ATAGAGGAAGAGGATCGAGCCCTCTTACTGCGAATCCTGGAAAGCATTGAAGACTTTGCT CAAGAACTAGTTGAATGCAAATCAAGCAGAGGGAGCCTATCACAAGAAAAGGAAATGATG CAAATTCTACAGGAAACCTTGACAACTTCCTCCCAGGCCAATTTATCAGTCTGTAGAAGT CCTGTTGGTGATAAAGCCAAAGATACTACTTCAGCAGTTTTGATTCAGCAGACTCCAGAG GTGATCAAGATTCAAAATAAACCAGAAAAGAAACCTGGAACACCACTCCCACCTCCAGCC ACCTCTCCAAGTAGTCCCCGACCTCTCTCCCCGGTTCCCCATGTGAATAATGTTGTGAAT GCGCCATTGTCCATAAACATTCCACGGTTCTACTTTCCTGAAGGACTCCCAGATACCTGT AGTAATCATGAACAAACTCTAAGCAGAATTGAAACTGCTTTCATGGATATTGAAGAACAG AAAGCAGACATTTATGAAATGGGGAAAATTGCAAAGGTCTGTGGCTGTCCTCTCTATTGG AAAGCCCCCATGTTCAGGGCTGCAGGGGGAGAGAAGACAGGATTTGTGACAGCACAGTCA TTCATTGCCATGTGGAGAAAGTTGCTGAATAACCATCATGATGATGCCTCTAAATTCATC TGTCTTCTAGCAAAGCCCAACTGCAGCTCTCTAGAACAGGAGGATTTCATCCCTCTACTT CAGGATGTGGTGGATACCCACCCTGGTCTCACGTTCCTGAAAGATGCTCCAGAATTCCAC TCCCGCTACATCACCACGGTTATTCAGAGAATATTCTACACAGTCAACAGATCTTGGAGT GGAAAAATTACTTCGACAGAGATAAGAAAAAGCAACTTTTTGCAAACCCTAGCACTTTTG GAAGAAGAGGAAGATATAAACCAAATTACAGATTACTTCTCCTATGAACATTTCTATGTT ATTTATTGTAAATTCTGGGAACTAGATACTGATCACGACCTCTACATCAGCCAGGCCGAT CTGTCTCGATACAATGACCAGGCTTCATCAAGCAGGATTATTGAAAGGATATTCTCTGGT GCAGTAACAAGGGGAAAAACAATACAGAAAGAGGGAAGAATGAGCTATGCAGATTTTGTT TGGTTTTTGATCTCTGAAGAAGACAAAAGGAATCCTACCAGCATTGAGTATTGGTTCCGC TGCATGGATGTGGATGGAGACGGTGTACTCTCCATGTATGAGCTGGAGTACTTCTATGAG GAGCAGTGTGAACGGATGGAAGCCATGGGAATTGAGCCCTTGCCATTCCATGATTTACTG TGCCAGATGCTTGACCTAGTGAAGCCAGCTGTTGATGGCAAAATAACTCTAAGAGATCTG AAGAGGTGCAGAATGGCTCACATCTTCTATGACACTTTCTTTAATCTGGAGAAATACTTA GACCATGAACAGAGAGATCCCTTTGCGGTCCAGAAGGATGTTGAGAACGATGGGCCTGAG CCCTCAGACTGGGACCGGTTTGCCGCTGAGGAGTATGAGACGCTTGTTGCAGAGGAATCT GCCCAAGCACAATTCCAGGAAGGCTTTGAAGATTATGAAACAGATGAACCTGCCTCTCCC TCTGAATTTGGAAACAAAAGCAATAAAATATTAAGTGCAAGCCTTCCAGAGAAATGTGGA AAGCTTCAATCAGTGGATGAAGAATAGCTGCCGGTGTCTACAATGAAACGAAGATGTGTA TTTTAAATGTTTCTTTCTTGTGAAGAGATGTTCTCGTTTGCATACTGCTTTTTAAAGACT TTGATTTCTCCAAGTGTGTATCATCTGCACTAGGAACTTTGTTTTTAAGCAATAGGTCTG GATACACATTTAACTTA >OriGene 5' read for NM_002718 unedited
TCACTATAGGCGGCCGCGNAATTCGCACGAGGGGGCCTGCGGGTGGCTGGGCGGGGCGCG CTCGGGCCTCCGCTCTTCACGCGCCGCATTCGTAGCCCGAGAGTCCGCGCCGCGGGGAGG CTTGGAGGCAAGCGCTGCCCGCGAGCTGAGCCGCCGGAGGAGGAGCCGCGGGCAACGAGG TTTCTCTGTCATTCACAGAAAAATGAATCATTTAAACCTTTGGAGGACTCAGTTATCACA ATACTTCTCTACTACCAACTAAGATTTATGATAGTAAATTTATGAGAGCAAATTTCCATG TTATAGAACTGTTGAAGAACTAAAAGAGGATATTCTTTCATCAAAATAATTCTGCAGTAT CATAATATTACTAAATAAAATTAAAAAGCACAATTATTAAATTATTAAATTTGCCACACA TGTGCAGCAGCTACTGTATCCTGATAGTGACCAAACCTCAAATATAAATGGTTTCCCTTC ATGGGAAAAGCCATTATATTTGGAAGAAACCACTGAACATTGTTATTAAATATATTTTCA GCTAACTGTCATTTAGGAGAATTTTCATGAAACAAGTTCTAGAAAGTTCCAAGTCCCACC AGTAAGTGGATTTGATATTATGGCAGCAACTTACAGACTTGTGGTTAGTACTGTGAACCA CTACAGCAGCGTGGTGATAGACCGGCGTTTTGAACAAGCTATACATTATTGCACTGGAAC CTGCCACACCTTCACACATGGAATTGACTGCATTGTGGTACACCATAGTGTTTGTGCAGA CCCTCTGCACATCCCTGTGNTCTCAGTCAAGATGCAGATCTGAACTCTATGTTTCTACCC ATGAAAAGGGGNCTTCTTCGCTGAGGAGACTATCCCAC >OriGene 3' read for NM_002718 unedited
ATGGTTCCACCAGGGCATGCCCACCCCGGGTATCTTGTTTCAGCAAACACCTATGAACCG CCGGCCCGCCAATCCTAAATCCGAGTTTTTTTTTTTTTTTTTTGTATTTCTATCTGAAAT GAAATAATTGCTTTTCTTCCCTGGTAAAGGCTGGATGGATTGGTAAGAGATTTCATTTAT CACATAAATAATCTTAATCTTAATAATTTATCAGTGTGTTTAGATTTTGAATATTGTGTT TCCTAAAAATTATTTTTAAGCTTTTAATTCTTTTGTGTGAACTATTAGATAGTTGCAATA GTTACTGCTTTCTGTAATAGTTAAATGGAAGATCAGTTCTAATCATGAAGATAAAATGAC AGGGAACTGGGAGCTTGTGACAGGCAGGATTCCTAGGATTGTTAACTCTCACTGTACTGT ACCTTGTTTATAGCATATTTATTTCCTAAATTCTCCTTTTCTTTTTGCCTACCATAACTC TAACCCAAGGTTCATTTTACTGCTTTGCCTTTATGCCGCCAGGAAGGAACCTAGTTAGGG TGGAAAGGGGANCCTATCCTTGCCCTTTCANACCTGTTTTAANAAAATTTCAGGCGAAAA ATTTANGTCCGGCCGAAGGNTNTTTATTTTTCTTGGGNNTTTTTCCCATTACNGNANGAT CNNCCCCTTTTNTTATNCGCACTTTACTGCAACNCATTGAGTTATCAAGATCTCTNATCG CACCTGCCAGTTCGANNTACTTGTGCGTACTANAACATATTTCNAGNCCTATATCTCTCC TGGTATCTAAGATATATATAGTTACTCCCTATGCNGAAATCGCGACACAGACAACTAATT TTCTTTCATTTATGGCTCATAAGACCTCATNCGACGCTCTACGTTCAT |
Restriction Sites | NotI-NotI |
ACCN | NM_002718 |
Insert Size | 6000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002718.3, NP_002709.2 |
RefSeq Size | 6762 bp |
RefSeq ORF | 3453 bp |
Locus ID | 5523 |
UniProt ID | Q06190 |
Domains | EFh |
Protein Families | Druggable Genome, Phosphatase |
Gene Summary | This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1), also known as isoform beta or isoform PR130. |
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Other Versions
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RC209027 | PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 12,008.00 |
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RC209027L3 | Lenti-ORF clone of PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 10,260.00 |
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RC209027L4 | Lenti-ORF clone of PPP2R3A (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 10,260.00 |
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RG209027 | PPP2R3A (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 1 |
CNY 9,220.00 |
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