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Cytochrome P450 3A4 (CYP3A4) (NM_017460) Human Untagged Clone
CAT＃: SC125488
CYP3A4 (untagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1
CNY 5,632.00
| Cited in 5 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CP33; CP34; CYP3A; CYP3A3; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1; VDDR3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_017460 edited
CGGCCGCGAATTCGGCACGAGAAGCTCCATGCACATAGCCCAGCAAAGAGCAACACAGAG CTGAAAGGAAGACTCAGAGGAGAGAGATAAGTAAGGAAAGTAGTGATGGCTCTCATCCCA GACTTGGCCATGGAAACCTGGCTTCTCCTGGCTGTCAGCCTGGTGCTCCTCTATCTATAT GGAACCCATTCACATGGACTTTTTAAGAAGCTTGGAATTCCAAGGCCCACACCTCTGCCT TTTTTGGGAAATATTTTGTCCTACCATAAGGGCTTTTGTATGTTTGACATGGAATGTCAT AAAAAGTATGGAAAAGTGTGGGGCTTTTATGATGGTCAACAGCCTGTGCTGGCTATCACA GATCCTGACATGATCAAAACAGTGCTAGTGAAAGAATGTTATTCTGTCTTCACAAACCGG AGGCCTTTTGGTCCAGTGGGATTTATGAAAAGTGCCATCTCTATAGCTGAGGATGAAGAA TGGAAGAGATTACGATCATTGCTGTCTCCAACCTTCACCAGTGGAAAACTCAAGGAGATG GTCCCTATCATTGCCCAGTATGGAGATGTGTTGGTGAGAAATCTGAGGCGGGAAGCAGAG ACAGGCAAGCCTGTCACCTTGAAAGACGTCTTTGGGGCCTACAGCATGGATGTGATCACT AGCACATCATTTGGAGTGAACATCGACTCTCTCAACAATCCACAAGACCCCTTTGTGGAA AACACCAAGAAGCTTTTAAGATTTGATTTTTTGGATCCATTCTTTCTCTCAATAACAGTC TTTCCATTCCTCATCCCAATTCTTGAAGTATTAAATATCTGTGTGTTTCCAAGAGAAGTT ACAAATTTTTTAAGAAAATCTGTAAAAAGGATGAAAGAAAGTCGCCTCGAAGATACACAA AAGCACCGAGTGGATTTCCTTCAGCTGATGATTGACTCTCAGAATTCAAAAGAAACTGAG TCCCACAAAGCTCTGTCCGATCTGGAGCTCGTGGCCCAATCAATTATCTTTATTTTTGCT GGCTATGAAACCACGAGCAGTGTTCTCTCCTTCATTATGTATGAACTGGCCACTCACCCT GATGTCCAGCAGAAACTGCAGGAGGAAATTGATGCAGTTTTACCCAATAAGGCACCACCC ACCTATGATACTGTGCTACAGATGGAGTATCTTGACATGGTGGTGAATGAAACGCTCAGA TTATTCCCAATTGCTATGAGACTTGAGAGGGTCTGCAAAAAAGATGTTGAGATCAATGGG ATGTTCATTCCCAAAGGGGTGGTGGTGATGATTCCAAGCTATGCTCTTCACCGTGACCCA AAGTACTGGACAGAGCCTGAGAAGTTCCTCCCTGAAAGATTCAGCAAGAAGAACAAGGAC AACATAGATCCTTACATATACACACCCTTTGGAAGTGGACCCAGAAACTGCATTGGCATG AGGTTTGCTCTCATGAACATGAAACTTGCTCTAATCAGAGTCCTTCAGAACTTCTCCTTC AAACCTTGTAAAGAAACACAGATCCCCCTGAAATTAAGCTTAGGAGGACTTCTTCAACCA GAAAAACCCGTTGTTCTAAAGGTTGAGTCAAGGGATGGCACCGTAAGTGGAGCCTGAATT TTCCTAAGGACTTCTGCTTTGCTCTTCAAGAAATCTGTGCCTGAGAACACCAGAGACCTC AAATTACTTTGTGAATAGAACTCTGAAATGAAGATGGGCTTCATCCAATGGACTGCATAA ATAACCGGGGATTCTGTACATGCATTGAGCTCTCTCATTGTCTGTGTAGAGTGTTATACT TGGGAATATAAAGGAGGTGACCAAATCAGTGTGAGGAGGTAGATTTGGCTCCTTTGCTTC TCACGGGACTATTTCCACCACCCCCAGTTAGCACCATTAACTCCTCCTGAGCTTTGATAA GAGAATCAACATTTCTCAATAATTTCCTCCCCAAATTATTAATGAAAATAAGAATTATTT TGATGGCTCTAACAATGACATTTATATCACATGTTTTCTCTGGAGTATTCTATAAGTTTT ATGTTAAATCAATAAAGACCCCTTTTCAAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAACTCG >OriGene 5' read for NM_017460 unedited
AAGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGANAAGCTCCAT GCACATAGCCCAGCAAAAAGCAACACAGAGCTGAAAGGAAGACTCAGAGGAGAGAGATAA GTAAGGAAAGTAGTGATGGCTCTCATCCCAGACTTGGCCATGGAAACCTGGCTTCTCCTG GCTGTCAGCCTGGTGCTCCTCTATCTATATGGAACCCATTCACATGGACTTTTTAAGAAG CTTGGAATTCCAAGGCCCACACCTCTGCCTTTTTTGGGAAATATTTTGTCCTACCATAAG GGCTTTTGTATGTTTGACATGGAATGTCATAAAAAGTATGGAAAAGTGTGGGGCTTTTAT GATGGTCAACAGCCTGTGCTGGCTATCACAGATCCTGACATGATCAAAACAGTGCTAGTG AAAGAATGTTATTCTGTCTTCACAAACCGGAGGCCTTTTGGTCCAGTGGGATTTATGAAA AGTGCCATCTCTATAGCTGAGGATGAAGAATGGAAGAGATTACGATCATTGCTGTCTCCA ACCTTCACCAGTGGAAAACTCAAGGAGATGGTCCCTATCATTGCCCAGTATGGAGATGTG TTGGTGAGAAATCTGAGGCGGGAAGCAGAGACAGGCAAGCCTGTCACCTTGAAAGACGTC TTTGGGGCCTACAGCATGGATGTGATCACTAGCACATCATTTGGAGTGAACATCGACTCT CTCAACAATCCACAAGACCNCTTTGTGGAAAACACCAAGAAGCTTTTAAGATTTGATTTT TTGGATCCATTCTTTCTCTCATAACAGTCTTTCCATTCCTCATCCNNNATTCTGNAGTAT TAAATATCTGTGTGNTTTCAAGAGAAGTTACAAATTTTTTAAGAAAATCTGTAAAAGGAT GAAAGAAAGTCGCTCGAAGATCACN >OriGene 3' read for NM_017460 unedited
NNATTATTTTNACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTACTTTTGAAAAGGGGTCTTTATTGATTTAACATAAAACTTATAGAATACTCC AGAGAAAACATGTGATATAAATGTCATTGTTAGAGCCATCAAAATAATTCTTATTTTCAT TAATAATTTGGGGAGGAAATTATTGAGAAATGTTGATTCTCTTATCAAAGCTCAGGAGGA GTTAATGGTGCTAACTGGGGGTGGTGGAAATAGTCCCGTGAGAAGCAAAGGAGCCAAATC TACCTCCTCACACTGATTTGGTCACCTCCTTTATATTCCCAAGTATAACACTCTACACAG ACAATGAGAGAGCTCAATGCATGTACAGAATCCCCGGTTATTTATGCAGTCCATTGGATG AAGCCCATCTTCATTTCAGAGTTCTATTCACAAAGTAATTTGAGGTCTCTGGTGTTCTCA GGCACAGATTTCTTGAAGAGCAAAGCAGAAGTCCTTAGGAAAATTCAGGCTCCACTTACG GTGCCATCCCTTGACTCAACCTTTAGAACAACGGGTTTTTCTGGTTGAAGAAGTCCTCCT AAGCTTAATTTCAGGGGGATNCTGTGTTTCTTTACAAGGTTTGAAGGAGAAGTTCTGAAG GACTCTGATTAGAGCAAGTTTCATGTTCATGAGAGCAAACCTCATGCCAATGCAGTTTCT GGGTCCACTTCCAAGGGTGTGTATATGTAAGGATCTATNGTGNCCTTGNTCTTCTTGCTG AATCTTTCAGGGNAGAAACTCTCANGCTCTGTCAGTACTTTTGGTCACGGTGAAAGCATA NCTTGGAATCATCACACCACCCCTTTGGNAATGACATCCCCATGATCTCACATCTTTTTT GCAGACCTCTCAGTCTATAGCATTGGGAATATCTGACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017460 |
| Insert Size | 2150 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017460.3, NP_059488.2 |
| RefSeq Size | 2768 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 1576 |
| UniProt ID | P08684 |
| Domains | p450 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, P450, Transmembrane |
| Protein Pathways | Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam, erythromycin, and chloroquine. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2020] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Clobetasol Propionate Is a Heme-Mediated Selective Inhibitor of Human Cytochrome P450 3A5
,Wright, WC;Chenge, J;Wang, J;Girvan, HM;Yang, L;Chai, SC;Huber, AD;Wu, J;Oladimeji, PO;Munro, AW;Chen, T;,
J. Med. Chem.
,PubMed ID 31965799
[CYP3A4]
|
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Enzymatic Activities of CYP3A4 Allelic Variants on Quinine 3-Hydroxylation In Vitro
,Zhou, XY;Hu, XX;Wang, CC;Lu, XR;Chen, Z;Liu, Q;Hu, GX;Cai, JP;,
Front Pharmacol
,PubMed ID 31214030
[CYP3A4]
|
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A Naturally Occurring Isoform-Specific Probe for Highly Selective and Sensitive Detection of Human Cytochrome P450 3A5
,Wu, JJ;Cao, YF;Feng, L;He, YQ;Hong, JY;Dou, TY;Wang, P;Hao, DC;Ge, GB;Yang, L;,
J. Med. Chem.
,PubMed ID 28441491
[CYP3A4]
|
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Transport and uptake of clausenamide enantiomers in CYP3A4-transfected Caco-2 cells: An insight into the efflux-metabolism alliance
,Hua, F;Shi, MJ;Zhu, XL;Li, M;Wang, HX;Yu, XM;Li, Y;Zhu, CJ;,
Biochem. Pharmacol.
,PubMed ID 26301745
[CYP3A4]
|
|
Cytochrome P450 3A5 Plays a Prominent Role in the Oxidative Metabolism of the Anti-Human Immunodeficiency Virus Drug Maraviroc
,Yanhui Lu, Craig W. Hendrix, and Namandjé N. Bumpus,
Drug Metab. Dispos., Dec 2012; 40: 2221 - 2230.
[CYP3A4]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210170 | CYP3A4 (Myc-DDK-tagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1 |
CNY 5,616.00 |
|
| RC210170L1 | Lenti ORF clone of Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1, Myc-DDK-tagged |
CNY 8,016.00 |
|
| RC210170L2 | Lenti ORF clone of Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210170L3 | Lenti ORF clone of Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1, Myc-DDK-tagged |
CNY 8,016.00 |
|
| RC210170L4 | Lenti ORF clone of Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1, mGFP tagged |
CNY 8,016.00 |
|
| RG210170 | CYP3A4 (tGFP-tagged) - Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1 |
CNY 7,216.00 |
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