SAP97 (DLG1) (NM_004087) Human Untagged Clone

CAT#: SC125478

DLG1 (untagged)-Human discs, large homolog 1 (Drosophila) (DLG1), transcript variant 2



  "NM_004087" in other vectors (6)

CNY 7,220.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (3)
DLG1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DLGH1; hdlg; SAP-97; SAP97
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC125478 representing NM_004087.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCACCTTTTGGAGGAATATCGTTCAAAACTAAGC
CAAACTGAAGACAGACAGCTCAGAAGTTCCATAGAACGGGTTATTAACATATTTCAGAGCAACCTCTTT
CAGGCTTTAATAGATATTCAAGAATTTTATGAAGTGACCTTACTGGATAATCCAAAATGTATAGATCGT
TCAAAGCCGTCTGAACCAATTCAACCTGTGAATACTTGGGAGATTTCCAGCCTTCCAAGCTCTACTGTG
ACTTCAGAGACACTGCCAAGCAGCCTTAGCCCTAGTGTAGAGAAATACAGGTATCAGGATGAAGATACA
CCTCCTCAAGAGCATATTTCCCCACAAATCACAAATGAAGTGATAGGTCCAGAATTGGTTCATGTCTCA
GAGAAGAACTTATCAGAGATTGAGAATGTCCATGGATTTGTTTCTCATTCTCATATTTCACCAATAAAG
CCAACAGAAGCTGTTCTTCCCTCTCCTCCCACTGTCCCTGTGATCCCTGTCCTGCCAGTCCCTGCTGAG
AATACTGTCATCCTACCCACCATACCACAGGCAAATCCTCCCCCAGTACTGGTCAACACAGATAGCTTG
GAAACACCAACTTACGTTAATGGCACAGATGCAGATTATGAATATGAAGAAATCACACTTGAAAGGGGA
AATTCAGGGCTTGGTTTCAGCATTGCAGGAGGTACGGACAACCCACACATTGGAGATGACTCAAGTATT
TTCATTACCAAAATTATCACAGGGGGAGCAGCCGCCCAAGATGGAAGATTGCGGGTCAATGACTGTATA
TTACGAGTAAATGAAGTAGATGTTCGTGATGTAACACATAGCAAAGCAGTTGAAGCGTTGAAAGAAGCA
GGGTCTATTGTACGCTTGTATGTAAAAAGAAGGAAACCAGTGTCAGAAAAAATAATGGAAATAAAGCTC
ATTAAAGGTCCTAAAGGTCTTGGGTTTAGCATTGCTGGAGGTGTTGGAAATCAGCATATTCCTGGGGAT
AATAGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACATAAGGATGGCAAACTTCAGATTGGA
GATAAACTTTTAGCAGTGAATAACGTATGTTTAGAAGAAGTTACTCATGAAGAAGCAGTAACTGCCTTA
AAGAACACATCTGATTTTGTTTATTTGAAAGTGGCAAAACCCACAAGTATGTATATGAATGATGGCTAT
GCACCACCTGATATCACCAACTCTTCTTCTCAGCCTGTTGATAACCATGTTAGCCCATCTTCCTTCTTG
GGCCAGACACCAGCATCTCCAGCCAGATACTCCCCAGTTTCTAAAGCAGTACTTGGAGATGATGAAATT
ACAAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACGGGCCTTGGTTTCAACATTGTAGGAGGA
GAAGATGGAGAAGGAATATTTATTTCCTTTATCTTAGCCGGAGGACCTGCTGATCTAAGTGGAGAGCTC
AGAAAAGGAGATCGTATTATATCGGTAAACAGTGTTGACCTCAGAGCTGCTAGTCATGAGCAGGCAGCA
GCTGCATTGAAAAATGCTGGCCAGGCTGTCACAATTGTTGCACAATATCGACCTGAAGAATACAGTCGT
TTTGAAGCTAAAATACATGATTTACGGGAGCAGATGATGAATAGTAGTATTAGTTCAGGGTCAGGTTCT
CTTCGAACTAGCCAGAAGCGATCCCTCTATGTCAGAGCCCTTTTTGATTATGACAAGACTAAAGACAGT
GGGCTTCCCAGTCAGGGACTGAACTTCAAATTTGGAGATATCCTCCATGTTATTAATGCTTCTGATGAT
GAATGGTGGCAAGCCAGGCAGGTTACACCAGATGGTGAGAGCGATGAGGTCGGAGTGATTCCCAGTAAA
CGCAGAGTTGAGAAGAAAGAACGAGCCCGATTAAAAACAGTGAAATTCAATTCTAAAACGAGAGATAAA
GGGCAGTCATTCAATGACAAGCGTAAAAAGAACCTCTTTTCCCGAAAATTCCCCTTCTACAAGAACAAG
GACCAGAGTGAGCAGGAAACAAGTGATGCTGACCAGCATGTAACTTCTAATGCCAGCGATAGTGAAAGT
AGTTACCGTGGTCAAGAAGAATACGTCTTATCTTATGAACCAGTGAATCAACAAGAAGTTAATTATACT
CGACCAGTGATCATATTGGGACCTATGAAAGACAGGATAAATGATGACTTGATCTCAGAATTTCCTGAC
AAATTTGGATCCTGTGTTCCTCATACAACTAGACCAAAACGAGATTATGAGGTAGATGGAAGAGATTAT
CATTTTGTGACTTCAAGAGAGCAGATGGAAAAAGATATCCAGGAACATAAATTCATTGAAGCTGGCCAG
TATAACAATCATCTATATGGAACAAGTGTTCAGTCTGTACGAGAAGTAGCAGAAAAGGGCAAACACTGT
ATCCTTGATGTGTCTGGAAATGCCATAAAGAGATTACAGATTGCACAGCTTTACCCTATCTCCATTTTT
ATTAAACCCAAATCCATGGAAAATATCATGGAAATGAATAAGCGTCTAACAGAAGAACAAGCCAGAAAA
ACATTTGAGAGAGCCATGAAACTGGAACAGGAGTTTACTGAACATTTCACAGCTATTGTACAGGGGGAT
ACGCTGGAAGACATTTACAACCAAGTGAAACAGATCATAGAAGAACAATCTGGTTCTTACATCTGGGTT
CCGGCAAAAGAAAAGCTATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_004087
Insert Size 2781 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004087.2
RefSeq Size 5034 bp
RefSeq ORF 2781 bp
Locus ID 1739
UniProt ID Q12959
Domains SH3, PDZ, L27, Guanylate_kin, GuKc
Protein Families Druggable Genome
Protein Pathways T cell receptor signaling pathway
MW 103.3 kDa
Gene Summary This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018]
Transcript Variant: This variant (2) contains an alternate in-frame coding exon compared to variant 1, resulting in a longer isoform (2) compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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