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Osteopontin (SPP1) (NM_000582) Human Untagged Clone
CAT＃: SC125352
SPP1 (untagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 2
CNY 2,400.00
CNY 2,950.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BNSP; BSPI; ETA-1; OPN |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125352 sequence for NM_000582 edited (data generated by NextGen Sequencing)
ATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCAGTTAAA CAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTG GCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTT CCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGAC GACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACT GATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACT GATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGAC ACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTT CGCAGACCTGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAA AGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCT TCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGT GCTGAAACCCACAGCCACAAGCAGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGC AATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCAC AGCCATGAATTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAA GATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGTCAAT TAA Clone variation with respect to NM_000582.2 240 t=>c >OriGene 5' read for NM_000582 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAGCATCGTCGGG ACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTC ACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCA GTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGAT GCTGTGGCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAG ACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGAT GATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGAT GACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTG GTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACA GTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAG AAGTTTCGCAGACCTGACATCCAGTACCCTGATGCTACAGACGANGACATCACCTCACAC ATGGAAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGACCTNGAC GCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCACCTGAATGAC CAGAGTGCTGAAACCCCCAGCCCCAACCAGTCCAGAATATATAACCGGGAAGCCCATGAT GAGAGCCATTGACCTTCCCGATGTGATTGATAGTCAGGAAACTTTCCAAAGTCGCCCGGA ATTCCCCACCCTGAATCTCC >OriGene 3' read for NM_000582 unedited
GTTGCGGCTCAGGGGATGGTCACTTCCCAGTTCCGGGAAAGCACTGAGGCAGGGGCTCAC AGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGT CGAGTTTTTTTTTTTTTTTTTTGCATTCAAGATAAAAGATTTATTCACACCACAAAAAGA TAATCACAACAAAATATACACTAACTTAAAAAACAAAAGATTATAGTGTCTTAAAATGTT ATATTCTCTTTTTAAGTGGGTAAAAGTATTTTGTTTGCTTCTACATAAATTTCTATTCGT GAGAGAATAACAAATATTAAAATACAGTGATAGTTTGCATTTCTTCTATAGAATGAACAT AGACATAACCCTGAAGCTTTTAGTTTACAGGGAGTTTCCATGAAGCCACAAACTAAACTA ATTATCAAACACATTAGTTATTTCCAGACTCAAATAGATACACATTCAACCAATAAACTG AGAAAGAAGCATTTCATGTTCTCTTTCATTTTGCTATAAAGCATTTTTTCTTTTGACTAA ATGCAAAGAGAGAAATTGTATTTTTTCTCCTTTTAATTGACCTCAGAAGATGCACTATCT AATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTACTTTTGGGGTCTACA ACCAGCATATCTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA AGTTCCTGACTATCAATCACATCGGAATGCTCATTGCTCTCATCATTGGCTTTCCGCTTA TATAATCTGGACTGCTTGTGGCTGTGGGTTTCAGCACTCTGGTCATCCAGCTGACTCGTT TCATAACTGTCCCTCCCACGGGTGGCCCAATCATAAAGCGCGTTCAGGTTCCTGAGCAAC GGGGATGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000582 |
| Insert Size | 1460 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000582.2, NP_000573.1 |
| RefSeq Size | 1616 bp |
| RefSeq ORF | 903 bp |
| Locus ID | 6696 |
| UniProt ID | P10451 |
| Domains | OSTEO |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 5. The resulting isoform (OPN-b, as described in PubMed: 7837791) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform e. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Noncontiguous Protein Interaction Domains in Osteopontin Contribute to Enhance HIV-1 Replication
,null,
Journal of human virology & retrovirology
,PubMed ID 31773048
[SPP1]
|
|
Lung cancer osteopontin isoforms exhibit angiogenic functional heterogeneity
,Justin D. Blasberg, Chandra M. Goparaju, Harvey I. Pass, and Jessica S. Donington,
J. Thorac. Cardiovasc. Surg., Jun 2010; 139: 1587 - 1593
[SPP1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205118 | SPP1 (Myc-DDK-tagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 2,400.00 |
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| RC205118L1 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
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| RC205118L2 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC205118L3 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205118L4 | Lenti ORF clone of Human secreted phosphoprotein 1 (SPP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205118 | SPP1 (tGFP-tagged) - Human secreted phosphoprotein 1 (SPP1), transcript variant 2 |
CNY 4,000.00 |
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