United States
Germany
Japan
United Kingdom
China
DNMT3B (NM_175848) Human Untagged Clone
CAT＃: SC124706
DNMT3B (untagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2
CNY 6,112.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ICF; ICF1; M.HsaIIIB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_175848, the custom clone sequence may differ by one or more nucleotides
ATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCA ACGGGGCCTGCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAG AGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTGTCCAGTCTGCTAAGCTACACACAGGACTTG ACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAAA CCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCA CAGGCCTTCCCCACGTTCCACCCGAGGCCGGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTC CCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCT CTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCC CTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGA GATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCA AGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGG CATGCGGTGGGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTG GGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAAGCCATGTACC ATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGGA CCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCC AACAACACGCAACCAGAGAACAAGACTCGAAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCC CCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCG AGAACAAATGGCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGG AAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTG AGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCT GCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACA GGCACAGCGGCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATG GCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGA ATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTG TTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTT CTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACGA CGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCA TGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCG AATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGA GAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATG ATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACA GGCCCGTGATAGCATCAAAGAATGATAAACTCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAA GTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCAACTTTTCCCT GTTGTCATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGC ACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCC TGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAG >OriGene 5' read for NM_175848 unedited
TTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGAGATTCGCGAGCCCA GCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCG CCCAGCCGCGCCCCAGCCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCT CAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCTGCAG CGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGG CCGAAGATCAAGCTCGCGACTCTCCAAGAGGGGAGGTGTCCAGTCTGCTAAGCTACACAC AGGACTTGACAGGCGATGGCGACGGGAAAGGGNNGGAATGGCTCTGACACCCCAGTCATG CCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGCCCAGCTTCCCTGAGACGGCGG GCAACAGCATCGGCAGGAACGCCATGGGCCGTCCCCTCCCAGCTCTTACCTTACCATCGA CCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGC CCGCCTAGCCCAGGACAGCCAGCAAGGGGGGCATGGAGCCCCCGCAGGTGGAGGCAGACA GTGGGAGATGAAAACAGTTCAAAGATCAGGATGGGAAAGGGATTTGGAATTAGGGGACCC TGTGTGGGGAAAAGAACAAGGGCTTCTCTTGGTGGCCCCCTGTTGGGGGTCTTGAAAAGC CCCTCCCAACCAAAGGCTTTGTTTGCTTTCCGGGGGTCCANNTGTTTTGGNNATGCAATT TTTCCAGGTTTTTNNAACAAATTGTGGCCTGGGGNTTTTTTACCCCCATTTTTTTTNGCA CTCA >OriGene 3' read for NM_175848 unedited
TGGACCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTAAACAA ATACTGATTTTAATTAAACATAAGGTAAACTCTAGGCATCCGTCATCTTTCAGCCTAAAA ATTAGCAAAAACTGTTGAAACAAGGCACAGTTTTTTCCCCATATTTGTTACGTCGTGGCT CCAGTTACAAAAAAATTTTAATGAAAACGTTAAACATAAAAATAGAAGTTTGAGATTTTA AAAAGTGTATAAAAAGCCCCACAAAACTTGTCAACGTTGTTCCTTATTCTACAAAATAGC ACCAGTAAGAAGAGTAAAAGGTGTTAAAAACCATTATGACAGCATTTCTGAAATGCAGCT TGTCTGAATTCCCGTTCTCCCTAAAAACGACTTCTTATGGAATAAAAAAGGATTAAAAAA TCTCCAAAGGGAGCACCGAGCTTTGCAGTTTTCCCTGTCATCTCTCAGATGTGGGGAAGG TATGAGAAATGTATGTCTGTCCCTGACTGCTGTCACTGCCTCTGAGTTTAGTAAAAAGAT GAGAAATGAGGGTAGCAGACTTCTCATCTGGGGACCTGTGCCTGTGGAGGGTAGGTCTCC TGGAAGGGAATGGGTTGAGGGGGGGTTTTATCTAAGCTCTTGCTTTCACTCCTTTCTAGG GAAGAGGAGATCCGGGGAGCCTTCCTGCCTTCAAAATATGGCGCGTTAATTTAGAACTGA GACACCCTTGCCTGACCCCCAGAGGCTCTTTTGTATTACGCGGAGGTTATACCAAAAACG CCCCTCTCCTTGACCTCGTGGGCAATCATTTTTTTTTTTCATTAACCCCTCATCGACAAA CTCATTAGTCCGTCCATCCCCCCGCCTCCCCCCTCTTTCCCTCCTCCCTACTTTCGCTCC CCCCCTCGTTTTCTCTTCCCCCACCTCTCCTTAGTCCCCCCCCATCCCCTCCTTCCTCTC CCCATTCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_175848 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175848.1, NP_787044.1 |
| RefSeq Size | 4293 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 1789 |
| UniProt ID | Q9UBC3 |
| Protein Families | Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| Gene Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Isoform 2 is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221966 | DNMT3B (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2 |
CNY 6,104.00 |
|
| RC221966L3 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221966L4 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG221966 | DNMT3B (tGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2 |
CNY 7,890.00 |
