DEGS1 (NM_144780) Human Untagged Clone

CAT#: SC124544

DEGS1 (untagged)-Human degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) (DEGS1), transcript variant 2



  "NM_144780" in other vectors (5)

CNY 6,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-DEGS1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DEGS; Des-1; DES1; FADS7; MGC5079; MIG15; MLD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_144780, the custom clone sequence may differ by one or more nucleotides


ATGGGGAGCCGCGTCTCGCGGGAAGACTTCGAGTGGGTCTACACCGACCAGCCGCACGCCGACCGGCGCC
GGGAGATCCTGGCAAAGTATCCAGAGATAAAGTCCTTGATGAAACCTGATCCCAATTTGATATGGATTAT
AATTATGATGGTTCTCACCCAGTTGGGTGCATTTTACATAGTAAAAGACTTGGACTGGAAATGGGTCATA
TTTGGGGCCTATGCGTTTGGCAGTTGCATTAACCACTCAATGACTCTGGCTATTCATGAGATTGCCCACA
ATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCGCTGGTTTGGAATGTTTGCTAATCTTCCTATTGG
GATTCCATATTCAATTTCCTTTAAGAGGTATCACATGGATCATCATCGGTACCTTGGAGCTGATGGCGTC
GATGTAGATATTCCTACCGATTTTGAGGGCTGGTTCTTCTGTACCGCTTTCAGAAAGTTTATATGGGTTA
TTCTTCAGCCTCTCTTTTATGCCTTTCGACCTCTGTTCATCAACCCCAAACCAATTACGTATCTGGAAGT
TATCAATACCGTGGCACAGGTCACTTTTGACATTTTAATTTATTACTTTTTGGGAATTAAATCCTTAGTC
TACATGTTGGCAGCATCTTTACTTGGCCTGGGTTTGCACCCAATTTCTGGACATTTTATAGCTGAGCATT
ACATGTTCTTAAAGGGTCATGAAACTTACTCATATTATGGGCCTCTGAATTTACTTACCTTCAATGTGGG
TTATCATAATGAACATCATGATTTCCCCAACATTCCTGGAAAAAGTCTTCCACTGGTGAGGAAAATAGCA
GCTGAATACTATGACAACCTCCCTCACTACAATTCCTGGATAAAAGTACTGTATGATTTTGTGATGGATG
ATACAATAAGTCCCTACTCAAGAATGAAGAGGCACCAAAAAGGAGAGATGGTGCTGGAGTAA


>OriGene 5' read for NM_144780 unedited
GGGGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCGCCGCC
GCCGCCACCTCTGAGCAGCCGGCTGGGAGCGAGAGCCGACAGCTAGTCTGCAAGCCACCG
CTGTCGCCATGGGGAGCCGCGTCTCGCGGGAAGACTTCGAGTGGGTCTACACCGACCAGC
CGCACGCCGACCGGCGCCGGGAGATCCTGGCAAAGTATCCAGAGATAAAGTCCTTGATGA
AACCTGATCCCAATTTGATATGGATTATAATTATGATGGTTCTCACCCAGTTGGGTGCAT
TTTACATAGTAAAAGACTTGGACTGGAAATGGGTCATATTTGGGGCCTATGCGTTTGGCA
GTTGCATTAACCACTCAATGACTCTGGCTATTCATGAGATTGCCCACAATGCTGCCTTTG
GCAACTGCAAAGCAATGTGGAATCGCTGGTTTGGAATGTTTGCTAATCTTCCTATTGGGA
TTCCATATTCAATTTCCTTTAAGAGGTATCACATGGATCATCATCGGTACTTTGGAGCTG
ATGGCGTCGATGTAGATATTCCTACCGATTTTGAGGGCTGGTTCTTCTGTACCGCTTTCA
GAAAGTTTATATGGGTTATTCTTCAGCCTCTCTTTTATGCCTTTCGACCTCTGTTCATCA
ACCCCAAACCAATTACGTATCTGGAAGTTATCAATACCGTGGCACAGGTCACTTTTGACA
TTNTAATTTATTACTTTNTGGGGATTAAATCCTTAGTCTACATNGTGGCAGCATCTTTAC
TTGGCCTGNGTTTGCACCCAATTTCTGGACATTTTATAGCTGAGCATTACATGTNCTTAA
AGGGTCATGAAACTTACTCATATTATGGGCCTCTGAAATTACTTACCTTCAATGTGGGNT
ATCATNATGAACATCATA
>OriGene 3' read for NM_144780 unedited
GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTGAAGGGGGGAAATTCAGTAAGTACTTTATTTATGAGTAAGGCTGTACAGGATTCAGCA
GTGTCTAATTCGGGTCCCATATAGAAGTTAAAATTTTTAAATGAGTAATTCAGCTTACAG
GGAAATATATTTTCATCTCAATCTTTTATGCTATATCATACCTCAGGCTTTTTAAAAATT
ATTTTTGAGATGAAATTTTAAAGTTCCCCCGGTACCCAAGTTACCATATTATATAGTATA
AAGATAGGGAAATACAAAAATGTAACAAATACTAATACACTGGGATTAATAGCTGGTTAT
TAGCTTTTTAATAAAGCAAAACTTTATAGGGAAATAAACTAAAAATAGCTTTTTATTTTT
TATATATATTTATTTATTTTTGAGAAGGGGTCTTGCTCTGTTGCCCAGGGTGGAGTGCAG
TGGCACAATCTTGGGTCACTGCAGCCTCTGCCTCCGCCTCCTGGGTTCAAGCAATTCTCC
CACCTCAGCCTCCCATGTAGCTGGGATTACAGGCATGTGCCGCCACGCCCGGCTAATTTT
TGCATTTTTAGTAAAGATGGGGTTACACCGTGTTGGCCAGGCTGGTCTCGAACTCCTGAC
CTCAGGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTATAGGCATGAGCCACCG
TGCCTGGCGAAATAGCTTTTTAGAAGCTTNTTAATGATATGCTTATAAATGACAGACATG
AGTGAAACACCTCAATGACGATATACACCAGTCACAAGTTCCCTGTGAGTGAACTGAGCG
TACAGAGTCAGCTGACTGTTAAAAGCAGATCACTTTTTGTATAACGACCTTTATCTGAAA
TGTGCAGGGAGCTCTGACCTCACTGCCTCAAGTTATATGCAAAATCCATTTATATCTAGG
TTGGAGAAAATNTTTGACCTAAGT
Restriction Sites NotI-NotI     
ACCN NM_144780
Insert Size 5700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_144780.1, NP_659004.1
RefSeq Size 1681 bp
RefSeq ORF 972 bp
Locus ID 8560
Domains FA_desaturase
Protein Families Druggable Genome, Transmembrane
Protein Pathways Metabolic pathways, Sphingolipid metabolism
Gene Summary This gene encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. [provided by RefSeq, Mar 2010]
Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants encode the same isoform.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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