CRAT (NM_144782) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	carnitine acetyltransferase; CAT1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_144782, the custom clone sequence may differ by one or more nucleotides ATGTTAGCCTTCGCTGCCAGGACCGTGGTGAAGCCTCTGGGCTTCCTGAAGCCCTTCTCCTTGATGAAGG CTTCCAGCCGCTTCAAGGCACACCAGGATGCACTGCCACGGCTGCCCGTGCCCCCTCTCCAGCAGTCCCT GGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAGGAGGAGTGGGCCCACACCAAGCAGCTGGTG GATGAGTTTCAGGCCTCAGGAGGTGTAGGGGAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCCAGGAAGA CGGAGAACTGGCTGTCTGAGTGGTGGCTCAAGACCGCCTACCTCCAGTACCGCCAGCCTGTGGTCATCTA CTCGAGCCCAGGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAGCTCCGATTTGCTGCC AAACTCATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTGGAGTACCTGG GGGGGAAGCCACTGTGCATGAACCAGTACTATCAGATCTTGTCCTCCTGCCGAGTGCCGGGCCCCAAGCA GGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCCACGCACATCACCGTGGTACACAACTACCAG TTTTTTGAGCTGGATGTGTACCACAGTGACGGGACACCCCTCACTGCGGATCAGATCTTTGTGCAGCTGG AGAAGATCTGGAACTCATCCCTACAGACCAACAAGGAGCCTGTGGGCATCCTCACCTCCAACCACCGCAA CTCCTGGGCCAAGGCATACAACACCCTCATCAAAGACAAGGTGAACCGGGATTCCGTGCGCTCCATCCAG AAGAAACCCGAGCTTGTGCGGTCTCCCATGGTGCCCCTGCCCATGCCCAAGAAGCTGCGGTTCAACATCA CCCCCGAGATCAAGAGCGACATCGAGAAGGCCAAGCAGAACCTCAGCATCATGATCCAGGACCTGGATAT CACCGTGATGGTGTTCCACCATTTTGGAAAAGACTTCCCCAAGTCGGAGAAGCTAAGCCCAGATGCCTTC ATCCAGATGGCTTTGCAGCTGGCCTACTACAGGATCTACGGACAGGCATGTGCCACCTATGAAAGTGCCT CCCTGCGCATGTTTCACCTGGGCCGCACCGACACCATCCGCTCGGCTTCCATGGACTCACTCACCTTTGT CAAGGCCATGGATGACTCCAGCGTCACGGAGCACCAGAAGGTGGAGCTGCTGCGGAAGGCCGTGCAGGCC CACCGAGGCTACACCGACCGGGCCATCCGCGGGGAGGCCTTTGATCGACACCTGCTGGGCCTGAAGCTGC AGGCCATCGAGGACCTGGTGAGCATGCCCGACATCTTCATGGACACCTCCTACGCCATCGCCATGCACTT CCACCTCTCCACCAGCCAGGTCCCTGCCAAGACAGACTGTGTCATGTTCTTCGGGCCCGTGGTCCCCGAC GGCTACGGTGTCTGCTATAACCCCATGGAGGCCCACATCAACTTCTCCCTGTCGGCCTACAACAGCTGCG CGGAGACCAACGCCGCCCGCCTGGCGCATTACCTGGAGAAGGCGCTCCTGGACATGCGTGCCCTGCTGCA GAGCCACCCCCGGGCCAAGCTCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_144782 unedited CAAATTTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGGCTGCCTCTACC GGCTGGCGGCTGTAACTCAGCGACCTTGGCCCGAAGGCTCTAGCAAGGACCCACCGACCC CAGCCGCGGCGGCGGCGGCGCGGACTTTGCCCGGTGTGTGGGGCGGAGCGGACTGCGTGT CCGCGGACGGGCAGCGAAGATGTTAGCCTTCGCTGCCAGGACCGTGGTGAAGCCTCTGGG CTTCCTGAAGCCCTTCTCCTTGATGAAGGCTTCCAGCCGCTTCAAGGCACACCAGGATGC ACTGCCACGGCTGCCCGTGCCCCCTCTCCAGCAGTCCCTGGACCACTACCTGAAGGCGCT GCAGCCCATCGTGAGTGAGGAGGAGTGGGCCCACACCAAGCAGCTGGTGGATGAGTTTCA GGCCTCAGGAGGTGTAGGGGAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCCAGGAAGAC GGAGAACTGGCTGTCTGAGTGGTGGCTCAAGACCGCCTACCTCCAGTACCGCCAGCCTGT GGTCATCTACTCGAGCCCAGGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGG TCAGCTCCGATTTGCTGCCAAACTATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGAC AACGAGACCCTGCCCGTGGAGTACCTGNGGGGGGAAGCCACTGTGCATGAACCAGTACTA TCAGATCTTGTCCTNCTGCCGAGTGCCGGGCCCCAAGCAGGACACAGTCAGCAACTTCAG CAAGACCAAGAAGCCTNCCACGCACATCACCGTGTACACACTACCAGTTTTTGAGCTGGA TGTGTACACAGTGACGGGCACCCTCACTGCGNATCANATCTTTGTGCACTGGAGAAGATC TGGACTCANCCCTACAGACACAGGNNAGCTGTGGCATNNCTACTNCAACACNGNACTNCT GGGCCAGGGCATACACACCTCTAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_144782 unedited GGAATCTATGNACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAACACAATTCCCCTTTTTTG ATGACCTTCCACATTAATATCATACAGCAGGGGGGGGGCAGGAACCAAACTGAGTGGAAC CAAAGGCGGCTCCCTAGCAATGTTTTCCACAGGAACCCACCCCCAACGGAGGTGAAACCT CACACCCTGGCCCCTCGGCCCCAAAAAATCCTGTCTCCAGGTCCTGGGAGTTTGGGAAAC AGGGTACAAATGGGGGCCTGGTCATGGAATTGGCAAGGAATGGCCCGGGCTTAACTGGTG AAAAAAAAAACTTTTGCCAATCTCCTGCTTTGGAGGGCTGGTTCCCTTCCCCAAAAGAAG CCCCGGTGGAGGACCTGCCAGGGGCCCGGGCCTAAAGTCCTGGTTAACCTTTGGACTCAA CCCCAGGTTGGGCCCTGGCAGGGGCTGGGATGACCCACGGAAGGGACTTGTGCTGGGGCC CGCAAGCCCCCTGGAGGAAGCCGTCCCGGGCTCAATAAATTTGGGGGGACCAGGGAAAAG GGAACCCAGGAAGAGGGAACCCAGGGGCTGAGCCCTGGGGGGGGGCCCATCCCAAGGGGG GCTTGGCTGTGGCATTGGCAGGCCTGAATCCTAAGGGCTCAAAACTGGCCCCGGGGTGGC TCTTCCACAGGGACCCCTGTTCCAGAACGCCCTTTCCAAGTAAAGCCCCCGCCGGCGGCG TTTGTCTTCCCCACCTTTTTTAGCCCAAGGGAAAAATTTTTGTGGCCCCCTGGGGTTAAA AAAAACCGTTGCCCCTGGGAACCCGGCCCAAAAAATAAAACATTTGTTTGGAAGGAACTG GTGGGGGAAA
Restriction Sites	NotI-NotI
ACCN	NM_144782
Insert Size	2800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_144782.1, NP_659006.1
RefSeq Size	2558 bp
RefSeq ORF	1635 bp
Locus ID	1384
Domains	Carn_acyltransf
Protein Families	Druggable Genome
Gene Summary	This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (3) lacks a segment in the coding region compared to variant 1. The translation remains in-frame, and results in an isoform (3) that lacks an internal region compared to isoform 1.

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