United States
Germany
Japan
United Kingdom
China
MBD1 (NM_015844) Human Untagged Clone
CAT＃: SC124480
MBD1 (untagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3
CNY 4,096.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CXXC3; PCM1; RFT |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_015844 edited
TGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGG GGGAGGTGCAGGAGGGACCCTCGCCATGGGTCCACGGGCCTAGAGTGGCGGAAGATACCG GCCTGGTGCCAAACTGGCTACTGCTGCTTCCTGTGGCCTCCATGGCTGAGGACTGGCTGG ACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAGTCAGGGGCCA CCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAG TTGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAG GCATCTTGTGCTATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAA AGAAGCCTTCAAGGCCAGCCAAGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGG TCAGGAAGGAGGCCCCGAGGGATGAGACCAAGGCTGACACTGACACAGCCCCAGCTTCAT TCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTCTCAGGGGATGGCACCCAAA GGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGAATTGCCTTCAACCGGG AACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTAACAGAAG ACTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGT TCTGCAAGTGTGAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAG TATGCCGGGGCTGTCAGACCCAAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTC CCCGCCCTGGTCTCAGGCGCCAGTGGAAATGTGTCCAGCGACGTTGCCTACGGGGTAAAC ATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGGCTGCCAGGCGGCGCCCCGGAGCCC AGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCCACAGAGCCGCACCCCAGAG CCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGTGTAGACGAGGACGAGCTAA AGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCGCCCCCACCTT ACCGTCGTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACCTTGAAGC CCACCTTGGCTACACGCACAGCCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAG CAGGTGGTGGCTTTGTGCTGCCCCCGCCTGGCACTGACCTTGTGTTTTTACGGGAAGGCG CAAGCAGTCCTGTGCAGGTGCCGGGCCCTGTTGCAGCTTCCACAGAAGCCCTGTTGCAGG AGGCCCAGTGCTCTGGCCTGAGTTGGGTTGTGGCCTTACCCCAGGTGAAGCAAGAGAAGG CGGATACCCAGGACGAGTGGACACCAGGCACAGCTGTCCTGACTTCTCCCGTATTGGTGC CTGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCTTCTGTGAAGCAAGAGCCACCTG ACCCAGAGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCCCCAGAGG AAGAGGCAGGAGGGGCTGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCC GCTTCCGAGATACAGCAGTCTGGTTGCCAAGGTCCAAAGACCTTAAAAAACCTGGAGCTA GAAAGCAGTAGACTGGAGGCTTCTACAGACTGTAGGATTCAAG:::::::GGCAGGCACT CGGGAAGGGAAGATGGATGTAAAGTGTGGGAGACCGAGGACACAGTGGAGCCCACGAGCA CGAGCTGGAACCCACGAGGATGGCCTGGAACCCATGTCAGTCTCTCACCACCTCCAGCTT CGATGATGTGGGTGTCCTGCAGAAGAAGCTGGTGCCCTTCCTCACAGAGTTAAATATGCA TCTGGCCCAGGAATTAGAGAAGCTGAAAGGATGATCCTGGGGAAGGTGGAGCAGCTGCAG GCCTGGCTGCAGGCCTGACTACTGCCCACACCAACGAGGTGATCTAGCAGATACATGGCA ACGTGTGAACTGCAACAACGCCTGGTGCCCCAGCACCAACCTTCCAAGTGTAAAAACAAT GTGCTGCTGCTTCACTTCCGCCCTCCGGTTATCAAGCAAAATGTCTCTTGTGGCCCATCT TACTGGAAGAGAGTTCCGGGAAACATAGCCTCACCAAGGTGACACATTACAAAGCCACCC TACCATGAATCCGCTCCCAAGGGTCTCACTGCTCACCTGAGGATAACTCAATATAACTAT GTTGCTGAAAATGCAAAGCTGAAGACCATGGATTTCATGGTGATTCCAGCAAGTACAGAG ATTCTATGAAGCCCACCCAGAAAAAACTTGCTGGTCCTGGCTATTTTTGTGTCATTTATT CAAGTATTGAGAACCTGGCCTGTGGTAGGCACTGTACTTAATACTAGGATACAGAAATGC AAAAGATACGGCCCATGCAATTTTATTAAATGCATCAATATGTATTACAAAAAAAAAAAA AAAAAAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015844 |
| Insert Size | 2600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_015844.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015844.1, NP_056669.1 |
| RefSeq Size | 2793 bp |
| RefSeq ORF | 1650 bp |
| Locus ID | 4152 |
| UniProt ID | Q9UIS9 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (3) lacks an in-frame coding exon compared to variant 1, resulting in a shorter isoform (3) missing an internal protein segment compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207708 | MBD1 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3 |
CNY 4,088.00 |
|
| RC207708L1 | Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, Myc-DDK-tagged |
CNY 6,488.00 |
|
| RC207708L2 | Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, mGFP tagged |
CNY 6,460.00 |
|
| RC207708L3 | Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207708L4 | Lenti ORF clone of Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3, mGFP tagged |
CNY 6,460.00 |
|
| RG207708 | MBD1 (tGFP-tagged) - Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3 |
CNY 5,040.00 |
