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SEMA6D (NM_153616) Human Untagged Clone
CAT＃: SC124441
SEMA6D (untagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 2
CNY 19,190.00
货期*
6周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_153616, the custom clone sequence may differ by one or more nucleotides
ATGAGGGTCTTCCTGCTTTGTGCCTACATACTGCTGCTGATGGTTTCCCAGTTGAGGGCAGTCAGCTTTC CTGAAGATGATGAACCCCTTAATACTGTCGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGACG CCCTTCAGGCAATGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTTTAT ATTGCTGGCAGGGATCAAGTTTATACAGTAAACTTAAATGAAATGCCCAAAACAGAAGTAATACCCAACA AGAAACTGACATGGCGATCAAGACAACAGGATCGAGAAAACTGTGCTATGAAAGGCAAGCATAAAGATGA ATGCCACAACTTTATCAAAGTATTTGTTCCAAGAAACGATGAGATGGTTTTTGTTTGTGGTACCAATGCA TTCAATCCCATGTGTAGATACTACAGGTTGAGTACCTTAGAATATGATGGGGAAGAAATTAGTGGCCTGG CAAGATGCCCATTTGATGCCAGACAAACCAATGTTGCCCTCTTTGCTGATGGGAAGCTGTATTCTGCCAC AGTGGCTGACTTCTTGGCCAGCGATGCCGTTATTTATCGAAGCATGGGTGATGGATCTGCCCTTCGCACA ATAAAATATGATTCCAAATGGATAAAAGAGCCACACTTTCTTCATGCCATAGAATATGGAAACTATGTCT ATTTCTTCTTTCGAGAAATCGCTGTCGAACATAATAATTTAGGCAAGGCTGTGTATTCCCGCGTGGCCCG CATATGTAAAAACGACATGGGTGGTTCCCAGCGGGTCCTGGAGAAACACTGGACTTCATTTCTAAAGGCT CGGCTGAACTGTTCTGTCCCTGGAGATTCGTTTTTCTACTTTGATGTTCTGCAGTCTATTACAGACATAA TACAAATCAATGGCATCCCCACTGTGGTCGGGGTGTTTACCACGCAGCTCAATAGCATCCCTGGTTCTGC TGTCTGTGCATTTAGCATGGATGACATTGAAAAAGTATTCAAAGGACGGTTTAAGGAACAGAAAACTCCA GATTCTGTTTGGACAGCAGTTCCCGAAGACAAAGTGCCAAAGCCAAGGCCTGGCTGTTGTGCAAAACACG GCCTTGCCGAAGCTTATAAAACCTCCATCGATTTCCCGGATGAAACTCTGTCATTCATCAAATCTCATCC CCTGATGGACTCTGCCGTTCCACCCATTGCCGATGAGCCCTGGTTCACAAAGACTCGGGTCAGGTACAGA CTGACGGCCATCTCAGTGGACCATTCAGCCGGACCCTACCAGAACTACACAGTCATCTTTGTTGGCTCTG AAGCTGGCATGGTACTTAAAGTTCTGGCAAAGACCAGTCCTTTCTCTTTGAACGACAGCGTATTACTGGA AGAGATTGAAGCCTACAACCATGCAAAGTGCAGTGCTGAGAATGAGGAAGACAAAAAGGTCATCTCATTA CAGTTGGATAAAGATCACCACGCTTTATATGTGGCGTTCTCTAGCTGCATTATCCGCATCCCCCTCAGTC GCTGTGAGCGTTATGGATCATGTAAAAAGTCTTGTATTGCATCTCGTGACCCGTATTGTGGCTGGTTAAG CCAGGGATCCTGTGGTAGAGTGACCCCAGGGATGCTTGCTGAAGGATATGAACAAGACACAGAATTCGGC AACACAGCTCATCTAGGGGACTGCCATGGTGTACGATGGGAAGTCCAGTCTGGAGAGTCCAACCAGATGG TCCACATGAATGTCCTCATCACCTGTGTCTTTGCTGCTTTTGTTTTGGGGGCATTCATTGCAGGTGTGGC AGTATACTGCTATCGAGACATGTTTGTTCGGAAAAACAGAAAGATCCATAAAGATGCAGAGTCCGCCCAG TCATGCACAGACTCCAGTGGAAGTTTTGCCAAACTGAATGGTCTCTTTGACAGCCCTGTCAAGGAATACC AACAGAATATTGATTCTCCTAAACTGTATAGTAACCTGCTAACCAGTCGGAAAGAGCTACCACCCAATGG AGATACTAAATCCATGGTAATGGACCATCGAGGGCAACCTCCAGAGTTGGCTGCTCTTCCTACTCCTGAG TCTACACCCGTGCTTCACCAGAAGACCCTGCAGGCCATGAAGAGCCACTCAGAAAAGGCCCATGGCCATG GAGCTTCAAGGAAAGAAACCCCTCAGTTTTTTCCGTCTAGTCCGCCACCTCATTCCCCATTAAGTCATGG GCATATCCCCAGTGCCATTGTTCTTCCAAATGCTACCCATGACTACAACACGTCTTTCTCAAACTCCAAT GCTCACAAAGCTGAAAAGAAGCTTCAAAACATTGATCACCCTCTCACAAAGTCATCCAGTAAGAGAGATC ACCGGCGTTCTGTTGATTCCAGAAATACCCTCAATGATCTCCTGAAGCATCTGAATGACCCAAATAGTAA CCCCAAAGCCATCATGGGAGACATCCAGATGGCACACCAGAACTTAATGCTGGATCCCATGGGATCGATG TCTGAGGTCCCACCTAAAGTCCCTAACCGGGAGGCATCGCTATACTCCCCTCCTTCAACTCTCCCCAGAA ATAGCCCAACCAAGCGAGTGGATGTCCCCACCACTCCTGGAGTCCCAATGACTTCTCTGGAAAGACAAAG AGGTTATCACAAAAATTCCTCCCAGAGGCACTCTATATCTGCTATGCCTAAAAACTTAAACTCACCAAAT GGTGTTTTGTTATCCAGACAGCCTAGTATGAACCGTGGAGGATATATGCCCACCCCCACTGGGGCGAAGG TGGACTATATTCAGGGAACACCAGTGAGTGTTCATCTGCAGCCTTCCCTCTCCAGACAGAGCAGCTACAC CAGTAATGGCACTCTTCCTAGGACGGGACTAAAGAGGACGCCGTCCTTAAAACCTGACGTGCCACCAAAG CCTTCCTTTGTTCCTCAAACCCCATCTGTCAGACCACTGAACAAATACACATACTAG >OriGene 5' read for NM_153616 unedited
TAGGCGGCCCGCGAATTCGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGGGATATCTA TTTTTACAATATTTTTCTTTATTTTCTTTCTTCCTTGCGCGTGGCAAGCTTTTTTTTTTC TTTTTCTTTTTTCTTACCAGCCTCCCCCCAATGAGGTAGCTCAGTGGCATTTCTGAGCAG GGGCCACCCTGACTTCACCTTGGCCCACCATGAGGGTCTTCCTGCTTTGTGCCTACATAC TGCTGCTGATGGTTTCCCAGTTGAGGGCAGTCAGCTTTCCTGAAGATGATGAACCCCTTA ATACTGTCGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGACGCCCTTCAGGCA ATGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTTTATA TTGCTGGCAGGGATCAAGTTTATACAGTAAACTTAAATGAAATGCCCAAAACAGAAGTAA TACCAAACAAGAAACTGACATGGCGATCAAGACTACAGGATCGAGAAAACTGTGCTATGA AAGGCAAGCATAAAGATGAATGCCACAACTTTATCAAAGTATTTGTTCCAAGAAACGATG AGATGGTTTTTGTTTGTGGTACCAATGCATTCAATCCCATGTGTAGATACTACAGGTTGA GTACCTTAGAATATGATGGGGAAAGAAATAGTGGCCTGGCAAGATGCCCATTTGATGCCC AGACAACCAATGTTGCCCTCTTTGCTGATGGGAGCTGTATTCTGCCCAGTGGCTGACTTC TTGCCAGCGATGCCGNTATTATCGAGCATGGTGATGGATCTGCCCTTCGCACATAAATAT GATTCCAATGGATAAAAGAGCACACTTCTTCTGCATAGATATGAAACTTGTCTATTCTCT TTCGAAACGCGTCGACATATAACATGCAGCTGGG >OriGene 3' read for NM_153616 unedited
TGAACATCGGTGATGGCAACTTCCAGGNCCAGNATAGCACTGGGGNAGGGGTCACAGGNA TGCCACCCGGGTATCTGTTCAGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAGGTAAACTCACATGTGGTATTTACAGTA TATTGTCATTACCTGCATGACTTAGGAAAAAATTCTCACATAACACAACACAGACAACAT GATTGCCCCACTGTTAGGTTTTTACAGACTGTTTCTGTTAACCCAATTACAATTCCTTTC ATATGTCTTTCTTTTGTCTTCTATTTTTGTTTGTTTGTTTGTTTTTTGTTTCTTACCTTG GGGGGTAGGAGGAGTTAAAGGAGGAAAAATTTTACTATTGGGGGGGGGGNGGAAACCAAC ACAATCAAAATAAAACCTTATTANTGTGTCCAAGGTTAAATANAGTGACTCAGGCTTTGA TTCACAGTGGACAGGAAAATTTTACTACTGAATACTGGAAAATAAATCAGGGATACCTCT TAATATCTTGATCTCCTTAACCTGGCCNGACTGGAATACAAGTTTTAATGAATAGCACCC CAAAATCCCTTGCAACTCTTACATTCTGGCCTTGGGGCCCAAATAAGTAAAGTTCATCAT TTTGATGGTCAAAGGACCCCCGATTCTATTCCCCTTTCAAAGCTGAACCCCATGGACTCC GGGGGAAAATAGAATTGCCCCCCGTTCCGCCCTGCTTAATGGAAACAACTCCCCTCCCCA AAAAAAACCAATGGGCACCTTAACCAACCCAAGGGGTACCAAACAAACCCCCCCCCTCTC CCACCGCCAATCCCCCCCGGCCGACCAAATCAAAAAAAAAAATTTATGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_153616 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153616.1, NP_705869.1 |
| RefSeq Size | 5884 bp |
| RefSeq ORF | 2997 bp |
| Locus ID | 80031 |
| UniProt ID | Q8NFY4 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Axon guidance |
| Gene Summary | Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (2) lacks two internal exons compared to variant 4. The encoded isoform (2) is missing an internal segment relative to isoform 4. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222173 | SEMA6D (Myc-DDK-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 2 |
CNY 7,130.00 |
|
| RC222173L3 | Lenti-ORF clone of SEMA6D (Myc-DDK-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 2 |
CNY 9,030.00 |
|
| RC222173L4 | Lenti-ORF clone of SEMA6D (mGFP-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 2 |
CNY 9,030.00 |
|
| RG222173 | SEMA6D (tGFP-tagged) - Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 2 |
CNY 7,890.00 |
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