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SEMA6D (NM_153617) Human Untagged Clone
CAT＃: SC124439
SEMA6D (untagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3
CNY 19,570.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_153617, the custom clone sequence may differ by one or more nucleotides
ATGAGGGTCTTCCTGCTTTGTGCCTACATACTGCTGCTGATGGTTTCCCAGTTGAGGGCAGTCAGCTTTC CTGAAGATGATGAACCCCTTAATACTGTCGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGACG CCCTTCAGGCAATGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTTTAT ATTGCTGGCAGGGATCAAGTTTATACAGTAAACTTAAATGAAATGCCCAAAACAGAAGTAATACCCAACA AGAAACTGACATGGCGATCAAGACAACAGGATCGAGAAAACTGTGCTATGAAAGGCAAGCATAAAGATGA ATGCCACAACTTTATCAAAGTATTTGTTCCAAGAAACGATGAGATGGTTTTTGTTTGTGGTACCAATGCA TTCAATCCCATGTGTAGATACTACAGGTTGAGTACCTTAGAATATGATGGGGAAGAAATTAGTGGCCTGG CAAGATGCCCATTTGATGCCAGACAAACCAATGTTGCCCTCTTTGCTGATGGGAAGCTGTATTCTGCCAC AGTGGCTGACTTCTTGGCCAGCGATGCCGTTATTTATCGAAGCATGGGTGATGGATCTGCCCTTCGCACA ATAAAATATGATTCCAAATGGATAAAAGAGCCACACTTTCTTCATGCCATAGAATATGGAAACTATGTCT ATTTCTTCTTTCGAGAAATCGCTGTCGAACATAATAATTTAGGCAAGGCTGTGTATTCCCGCGTGGCCCG CATATGTAAAAACGACATGGGTGGTTCCCAGCGGGTCCTGGAGAAACACTGGACTTCATTTCTAAAGGCT CGGCTGAACTGTTCTGTCCCTGGAGATTCGTTTTTCTACTTTGATGTTCTGCAGTCTATTACAGACATAA TACAAATCAATGGCATCCCCACTGTGGTCGGGGTGTTTACCACGCAGCTCAATAGCATCCCTGGTTCTGC TGTCTGTGCATTTAGCATGGATGACATTGAAAAAGTATTCAAAGGACGGTTTAAGGAACAGAAAACTCCA GATTCTGTTTGGACAGCAGTTCCCGAAGACAAAGTGCCAAAGCCAAGGCCTGGCTGTTGTGCAAAACACG GCCTTGCCGAAGCTTATAAAACCTCCATCGATTTCCCGGATGAAACTCTGTCATTCATCAAATCTCATCC CCTGATGGACTCTGCCGTTCCACCCATTGCCGATGAGCCCTGGTTCACAAAGACTCGGGTCAGGTACAGA CTGACGGCCATCTCAGTGGACCATTCAGCCGGACCCTACCAGAACTACACAGTCATCTTTGTTGGCTCTG AAGCTGGCATGGTACTTAAAGTTCTGGCAAAGACCAGTCCTTTCTCTTTGAACGACAGCGTATTACTGGA AGAGATTGAAGCCTACAACCATGCAAAGTGCAGTGCTGAGAATGAGGAAGACAAAAAGGTCATCTCATTA CAGTTGGATAAAGATCACCACGCTTTATATGTGGCGTTCTCTAGCTGCATTATCCGCATCCCCCTCAGTC GCTGTGAGCGTTATGGATCATGTAAAAAGTCTTGTATTGCATCTCGTGACCCGTATTGTGGCTGGTTAAG CCAGGGATCCTGTGGTAGAGTGACCCCAGGGATGCTTGCTGAAGGATATGAACAAGACACAGAATTCGGC AACACAGCTCATCTAGGGGACTGCCATGAAATTTTGCCTACTTCAACTACACCAGATTACAAAATATTTG GCGGTCCAACATCTGGTGTACGATGGGAAGTCCAGTCTGGAGAGTCCAACCAGATGGTCCACATGAATGT CCTCATCACCTGTGTCTTTGCTGCTTTTGTTTTGGGGGCATTCATTGCAGGTGTGGCAGTATACTGCTAT CGAGACATGTTTGTTCGGAAAAACAGAAAGATCCATAAAGATGCAGAGTCCGCCCAGTCATGCACAGACT CCAGTGGAAGTTTTGCCAAACTGAATGGTCTCTTTGACAGCCCTGTCAAGGAATACCAACAGAATATTGA TTCTCCTAAACTGTATAGTAACCTGCTAACCAGTCGGAAAGAGCTACCACCCAATGGAGATACTAAATCC ATGGTAATGGACCATCGAGGGCAACCTCCAGAGTTGGCTGCTCTTCCTACTCCTGAGTCTACACCCGTGC TTCACCAGAAGACCCTGCAGGCCATGAAGAGCCACTCAGAAAAGGCCCATGGCCATGGAGCTTCAAGGAA AGAAACCCCTCAGTTTTTTCCGTCTAGTCCGCCACCTCATTCCCCATTAAGTCATGGGCATATCCCCAGT GCCATTGTTCTTCCAAATGCTACCCATGACTACAACACGTCTTTCTCAAACTCCAATGCTCACAAAGCTG AAAAGAAGCTTCAAAACATTGATCACCCTCTCACAAAGTCATCCAGTAAGAGAGATCACCGGCGTTCTGT TGATTCCAGAAATACCCTCAATGATCTCCTGAAGCATCTGAATGACCCAAATAGTAACCCCAAAGCCATC ATGGGAGACATCCAGATGGCACACCAGAACTTAATGCTGGATCCCATGGGATCGATGTCTGAGGTCCCAC CTAAAGTCCCTAACCGGGAGGCATCGCTATACTCCCCTCCTTCAACTCTCCCCAGAAATAGCCCAACCAA GCGAGTGGATGTCCCCACCACTCCTGGAGTCCCAATGACTTCTCTGGAAAGACAAAGAGGTTATCACAAA AATTCCTCCCAGAGGCACTCTATATCTGCTATGCCTAAAAACTTAAACTCACCAAATGGTGTTTTGTTAT CCAGACAGCCTAGTATGAACCGTGGAGGATATATGCCCACCCCCACTGGGGCGAAGGTGGACTATATTCA GGGAACACCAGTGAGTGTTCATCTGCAGCCTTCCCTCTCCAGACAGAGCAGCTACACCAGTAATGGCACT CTTCCTAGGACGGGACTAAAGAGGACGCCGTCCTTAAAACCTGACGTGCCACCAAAGCCTTCCTTTGTTC CTCAAACCCCATCTGTCAGACCACTGAACAAATACACATACTAG >OriGene 5' read for NM_153617 unedited
TATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCTGACTTCACCTTGGCCCA CCATGAGGGTCTTCCTGCTTTGTGCCTACATACTGCTGCTGATGGTTTCCCAGTTGAGGG CAGTCAGCTTTCCTGAAGATGATGAACCCCTTAATACTGTCGACTATCACTATTCAAGGC AATATCCGGTTTTTAGAGGACGCCCTTCAGGCAATGAATCGCAGCACAGGCTGGACTTTC AGCTGATGTTGAAAATTCGAGACACACTTTATATTGCTGGCAGGGATCAAGTTTATACAG TAAACTTAAATGAAATGCCCAAAACAGAAGTAATACCAAACAAGAAACTGACATGGCGAT CAAGACAACAGGATCGAGAAAACTGTGCTATGAAAGGCAAGCATAAAGATGAATGCCACA ACTTTATCAAAGTATTTGTTCCAAGAAACGATGAGATGGTTTTTGTTTGTGGTACCAATG CATTCAATCCCATGTGTAGATACTACAGGTTGAGTACCTTAGAATATGATGGGGAAGAAA TTAGTGGCCTGGCAAGATGCCCATTTGATGCCAGACAAACCAATGTTGCCCTCTTTGCTG ATGGGAAGCTGTATTCTGCCACAGTGGCTGACTTCTTGGCCAGCGATGCCCGTATTTATC GAAGCATGGGTGATGGATTCTGCCCTTTCGCACAATAAAATATGATTCCAAATGGATAAA AGAGCCCACACTTTTCTTCATGCCATAGAATATGGGANACTATGTCTATTTTTCTTCTTT CGAGAAAATCGCTGGTCGAACATTATAATNTAAGGCAGGGCTGGGTATTCTCCCGGGTGG CCCGCATATGTAAAACGACACTGGGTGGGTTCCACCCGGGTCTGGAGAACAACTGGACTT CATTCTTAAGGCTCGCCTGACCGTTCTGTCCTGAGAATACGTTCTTCTCTTTGAGAGAGG GCCCA >OriGene 3' read for NM_153617 unedited
ACACATTGGCGATTGGCAACCTTCCAGGGNCCAGGGTGAGAGCACTGGAGGCAGGGGTAC ACAGGNGCATGCCACTCCGGGCATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATC TAGAGTCGAGTTTTTTTTTTTTTTTTTTAATGATTTGTATATTTTATTTTGACTTTACAT GCGGTGTTAAAAACCCAAAGTACATATTATTTACACATCCACAATACAAGTTTACAAGGT ATCTATTCATGTTAAAGTACTCTATAAAACAGTATAGAGACACTTCACTTGAGGGTTACT TTTTATGCCGTACCATTAAAAAAGATACAATCTGCGTTTACCTTTGCACAAATGGATAAA GCATTTAAAAATTAAATTAACAGGATAGAGACTAGATTTAATGTTAGAAATTTCAACATA AATACTGATTGAACTCACATTACAAACTACAAGGAAGCACAATGTTACAAAATGTTATCT GGGGTCACATAGAGTTACTGTTCTGTTTTACACACGCTGGGATTAATATTGGTAAGGTGA ATTCAAACACGTTTGTTGTTGGTGGACTGAGTAGTGTTTATCATTGGTTGGTTTAATTTA ATAATAAACTGGAATATTTTANTTGCCAAGCTGAAGTGTTTCAAGAACAGAAATTTTAGC TTTAGCGCTCCCAAAGATCACTGGAAAATCACGTTCTTAATAAAAAAATGTTTCTTAAAA AAAAAAAAAACTGGGATCAGNTTTCCCCATTACTGTAAAAAAATGATCTTGGTCCTCNGC AANATGTCCCCCCCCCAAATNNTAGGTCCCCACCGGATTAGCCAAAGAAAACCATTTGAA ACNCATTTACCTGATTACAGCNNNGGCAAAACCCNGGCGTGGGAATTTACAAGAATGGTT ACCCAAAAAAGTGAGTGGAGGACANNNAATTNACTGAAAAATGNNCAAAGTTCAAACAAT T |
| Restriction Sites | NotI-NotI |
| ACCN | NM_153617 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153617.1, NP_705870.1 |
| RefSeq Size | 5941 bp |
| RefSeq ORF | 3054 bp |
| Locus ID | 80031 |
| UniProt ID | Q8NFY4 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Axon guidance |
| Gene Summary | Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (3) lacks an internal exon, compared to variant 4. The encoded isoform (3) is missing an internal segment relative to isoform 4. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216341 | SEMA6D (Myc-DDK-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3 |
CNY 10,170.00 |
|
| RC216341L1 | Lenti-ORF clone of SEMA6D (Myc-DDK-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3 |
CNY 10,192.00 |
|
| RC216341L2 | Lenti-ORF clone of SEMA6D (mGFP-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3 |
CNY 12,070.00 |
|
| RC216341L3 | Lenti-ORF clone of SEMA6D (Myc-DDK-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3 |
CNY 12,070.00 |
|
| RC216341L4 | Lenti-ORF clone of SEMA6D (mGFP-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3 |
CNY 12,070.00 |
|
| RG216341 | SEMA6D (tGFP-tagged) - Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 3 |
CNY 11,210.00 |
